Comparative experimental study of the performance of bacterioscopic methods in detecting acid-fast bacilli: Ziehl-Neelsen microscopy, conventional fluorescence and led fluorescence microscopy
https://doi.org/10.25789/YMJ.2019.66.16
Abstract
Performance of bacterioscopic methods was comparatively studied in Bacteriologic Laboratory of the Phthisiatry Research-Practice Center, to assess the detection of acid-fast bacilli (AFB) by Ziehl-Neelsen (ZN) microscopy, conventional fluorescence microscopy (FM), and LED fluorescence microscopy (LED-FM). The results of the study showed a higher sensitivity of the FM, compared with the ZN method. Also, due to the fact that LED fluorescent microscopes do not require highly skilled maintenance and have a significantly longer lamp life compared to conventional models of fluorescent microscopes, the use of LED technology is justified from an economic point of view and makes it widely recommended for tuberculosis diagnosing.
About the Authors
N. G. Pavlov
Phthisiatry Research-Practice Center
Russian Federation
Russian Federation
Pavlov Nikolai Gerasimovich, Cand.Sci. (Veterinary Medicine), senior researcher, Bacteriological laboratory
Mobile: 79644240417
Yakutsk, Republic Sakha (Yakutia)
G. I. Alekseeva
Phthisiatry Research-Practice Center
Russian Federation
Russian Federation
Alekseeva Galina Ivanovna, Dr.Sci. (Medicine), Head of Bacteriological laboratory
Mobile: 79644260609
Yakutsk, Republic Sakha (Yakutia)
G. P. Protodiakonova
Yakutsk State Agricultural Academy
Russian Federation
Russian Federation
Protodiakonova Galina Petrovna, Dr.Sci. (Animal Medicine), Dean of Animal Medicine Faculty
Mobile: 79148235006
M. V. Chernykh
Phthisiatry Research-Practice Center
Russian Federation
Russian Federation
Chernykh Marina Valerievna, bacteriologist, Bacteriological laboratory
Mobile: 79142221306
Yakutsk, Republic Sakha (Yakutia)
E. I. Ivanova
Phthisiatry Research-Practice Center
Russian Federation
Russian Federation
Ivanova Elena Ivanovna, bacteriologist, Bacteriological laboratory
Mobile: 79141079431
Yakutsk, Republic Sakha (Yakutia)
M. V. Yakovleva
Phthisiatry Research-Practice Center
Russian Federation
Russian Federation
Yakovleva Mariya Vasilievna, medical biologist, Bacteriological laboratory
Mobile: 79142974248
Yakutsk, Republic Sakha (Yakutia)
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Review
For citations:
Pavlov N.G., Alekseeva G.I., Protodiakonova G.P., Chernykh M.V., Ivanova E.I., Yakovleva M.V. Comparative experimental study of the performance of bacterioscopic methods in detecting acid-fast bacilli: Ziehl-Neelsen microscopy, conventional fluorescence and led fluorescence microscopy. Yakut Medical Journal. 2019;(2):56-59. https://doi.org/10.25789/YMJ.2019.66.16
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