Obtaining a culture of primary fibroblasts from a skin biopsy
Abstract
In this paper we describe the method of isolation, maintaining and storage of human primary skin fibroblasts to treat damaged skin. The described method is fast, simple and reproducible.
About the Authors
V. A. Alekseev
Federal State Budget Scientific Institution “Yakutsk Scientific Center of Complex Medical Problems”
Russian Federation
Russian Federation
Alexeev Vladislav Amirovich - Junior Researcher of the Immunological Research Laboratory of the Department for Studying Adaptation Mechanisms
677000, Yakutsk, ul. Kalandarashvili 38/4, ap. 51
Tel .: +7 (996) 316-50-05
G. A. Apsolikhova
Federal State Budget Scientific Institution “Yakutsk Scientific Center of Complex Medical Problems”
Russian Federation
Russian Federation
Apsolikhova Galina Alexandrovna - Junior Researcher of the laboratory of hereditary pathology of the department of molecular genetics
677007, Yakutsk, ul. Krupskaya, 18
Tel .: +7 (999) 173-88-66
N. I. Pavlova
Federal State Budget Scientific Institution “Yakutsk Scientific Center of Complex Medical Problems”
Russian Federation
Russian Federation
Pavlova Nadezhda Ivanovna - Post-graduate student, Federal State Educational Establishment of Higher Education “Yakut State Agricultural Academy” Junior scientific employee of the laboratory of hereditary pathology of the department of molecular genetics of the Federal State Budget Scientific Institution “Yakutsk Scientific Center of Complex Medical Problems”
677000, Yakutsk, ul. Lisa Chaykina 3, Apt. 6th
Tel .: +7 (914) 289-39-36
T. S. Bobokova
ЯНЦ КМП
Russian Federation
Russian Federation
N. A. Solovieva
Federal State Budget Scientific Institution “Yakut Scientific Center of Complex Medical Problems”
Russian Federation
Russian Federation
Solovieva Natalia Alekseevna - Scientific Secretary
677000, Yakutsk, ul. Mordinova 21/1, ap. 42
Tel. +7 (924) 171-34-89
E. N. Popov
State autonomous institution of the Republic of Sakha (Yakutia) Technopark Yakutia”
Russian Federation
Russian Federation
Popov Evgeny Nikolaevich - Leading expert of the biotechnology laboratory
677000, Yakutsk, ul. Kalandarashvili 7, ap. 171
Tel. +7 (924) 469-48-61
Kh. A. Kurtanov
Federal State Budget Scientific Institution “Yakutsk Scientific Center of Complex Medical Problems”
Russian Federation
Russian Federation
Kurtanov Khariton Alekseevich - Candidate of Medical Sciences, Head of the Laboratory of Hereditary Pathology, Department of Molecular Genetics
677027, Yakutsk, ul. Petrovsky 32, apt. 66
Tel .: +7 (914) 106-00-30
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11.
Review
For citations:
Alekseev V.A., Apsolikhova G.A., Pavlova N.I., Bobokova T.S., Solovieva N.A., Popov E.N., Kurtanov Kh.A. Obtaining a culture of primary fibroblasts from a skin biopsy. Yakut Medical Journal. 2017;(1):44-46.
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