Approbation of the molecular genetic method for the diagnosis of Helicobacter pylori infection in Yakutia
Abstract
In this paper we presented the results of approbation of the PCR method for the diagnosis of Helicobacter pylori infection based on the amplification of the 16S rRNA marker gene of bacterial DNA isolated from samples of gastric mucosa tissue from patients with gastroduodenal diseases in Yakutia.
About the Authors
N. N. Gotovtsev
ЯНЦ КМП
Russian Federation
Russian Federation
N. A. Barashkov
ЯНЦ КМП
Russian Federation
Russian Federation
Т. V. Borisova
СВФУ им. М.К. Аммосова
Russian Federation
Russian Federation
M. V. Pak
ЯНЦ КМП; РБ№1-НЦМ
Russian Federation
Russian Federation
M. P. Alekseeva
ЯНЦКМП; РБ№1-НЦМ
Russian Federation
Russian Federation
N. N. Innokentieva
СВФУ им. М.К. Аммосова
Russian Federation
Russian Federation
K. S. Loskutova
ЯНЦКМП; РБ№1-НЦМ
Russian Federation
Russian Federation
V. G. Pshennikova
ЯНЦ КМП
Russian Federation
Russian Federation
A. M. Rafailov
СВФУ им. М.К. Аммосова
Russian Federation
Russian Federation
S. N. Lekhanova
СВФУ им. М.К. Аммосова
Russian Federation
Russian Federation
S. A. Fedorova
СВФУ им. М.К. Аммосова
Russian Federation
Russian Federation
References
1. Alexandrova S.L. Helicobacter pylori associated gastroduodenal pathology in children in the region of Yakutia / S.L. Aleksandrova, N.N. Barashkova, E.A. Kornienko // Siberian Journal of Medicine. – 2007. – P. 86-88.
2. Vasilev A.Yu. Analysis of the data of radiation research methods based on the principles of evidence-based medicine / A.Yu. Vasilev, A.Yu. Maliy, N.S. Serov: manual. – 2008. – P. 25.
3. Ilchishina T.A. Features of laboratory diagnostics of Helicobacter pylori and clinical course of chronic gastritis and peptic ulcer in case of bacillary-coccal dysmorphism of bacteria / T.A. Ilchishina: PhD thesis: 14.00.46, 14.00.47. – St. Petersburg, 2008. – P. 136.
4. Features of morphological picture, gastric acidity and contamination of H. pylori depending on the clinical course of gastric ulcer and duodenal ulcer / V.G. Krivoshapkin, T.N. Kurbatova // Far East Medical Journal. – 2007. – №4. – Р. 35-37.
5. Lekhanova S.N. Morphological characteristics of HP-associated gastritis in children and adolescents of Yakutia / S.N. Lekhanova, V.A. Argunov // Herald of NSU. Series: biology, clinical medicine. – 2009. – Vol. 7, №. 1. – P. 72-76.
6. Loskutova K.S. Changes in the mucous membrane of the antrum with Helicobacter pyloriassociated gastritis in the population of Yakutia / K.S. Loskutova // Herald of the North-Eastern Federal University M.K. Ammosov. – 2006. – Vol.3, №2. – Р.22-26.
7. Diagnostics methods of Helicobacter pylori infection: modern condition of issue / S.S. Bunova, L.B. Rybkina, I.A. Bakalov [et al.] // Young scientist. – 2012. – №12. – P. 540-543.
8. Development of the ideas of Academician V. Kh. Vasilenko in modern gastroenterology / O.A. Sklyanskaya, T.L. Lapina, L.P. Myagkova [et al.] // to the 96-th anniversary. – M., 1993. – Vol. 2. – С. 85-87.
9. Chronic gastritis / L.I. Aruin, P. Ya Grigorev, V.A. Isacov [et al.]. – Amsterdam, 1993. – P. 362.
10. A comparison of 16S ribosomal DNA sequences from five isolates of Helicobacter pylori / B.W. Eckloff, R.P. Podzorski, B.C. Kline [et al.] // Int J Syst Bacteriol. – 1994. – Apr. – Vol. 44. – № 2. – P. 320-323.
11. Comparison of PCR and other diagnostic techniques for detection of Helicobacter pylori infection in dyspeptic patients / J. Weiss, J. Mecca, E. da Silva [et al.] // J Clin Microbiol. – 1994. – Jul. – Vol. 32. – №7. – 1663-1668.
12. Efficacy of four widely used techniques of the diagnosis of Helicobacter pylori infection in gastric ulcer disease / S.J.F. Bermejo, M.D. Boixeda de, J. Gisbert [et al.] // Rev. Clin. Esp. – 2000. – Vol. 200. – P. 475-479.
13. Evaluation of CLO test and polymerase chain reaction for biopsy-dependent diagnosis of Helicobacter pylori infection / C.W. Lin, H.H. Wang, Y.F. Chang [et al.] // Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1997. – Nov. – Vol. 30. – №4. – Р. 219-227.
14. Helicobacter pylori factors involved in the development of gastroduodenal mucosal damage and ulceration / Y. Germani, C. Dauga, P. Duvat [et al.] // Res. Microbiol. – 1997. – Vol.148. – №4. – P. 315-326.
15. Helicobacter pylori strains are simultaneously present in the stomach of most patients with non-ulcer dyspepsia: relevance to histological damage / H. Cohen, L. Laine // Aliment. Pharmacol. Ther. – 1997. – Vol. 11. – Suppl. I. – P. 3-9.
16. Identification of Helicobacter pylori and the cagA genotype in gastric biopsies using highly sensitive real-time PCR as a new diagnostic tool / S. Yamazaki, S. Kato, N. Matsukura [et al.] // FEMS Immunol Med Microbiol. 2005. – Jun 1; 44(3): 261–268.
17. Lee J. Y. Diagnosis of Helicobacter pylori by invasive test: histology / J. Y. Lee, N. Kim // Annals of Translational Medicine. – 2015. – Jan. – Vol. 3. – №1. – 10. doi.org/10.3978/j.issn.2305-5839.2014.11.03
18. Montgomery E.A. Rapid diagnosis of Campylobacter pylori by Gram’s stain / E.A. Montgomery, D.F. Martin, D.A. Peura // Am J Clin Pathol 1988. – V. 90. – P. 606-609.
19. PCR-based diagnosis of Helicobacter pylori infection in Polish children and adults / Gzyl A., Dzierzanowska D., Rozynek E. [et al.] // J Med Microbiol. 1999. – Apr. – Vol. 48. – №4. – P. 349-56.
20. Performance of Routine Helicobacter pylori Invasive Tests in Patients with Dyspepsia / H. – C. Lee, T. – C. Huang, C. – L. Lin [et al.] // Gastroenterology Research and Practice. – 2013. – 184806. doi.org/10.1155/2013/184806
21. Real-Time PCR Improves Helicobacter pylori Detection in Patients with Peptic Ulcer Bleeding / M.J. Ramírez-Lázaro, S. Lario, A. Casalots [et al.] // PLoS ONE. – 2011. – Vol. 6. – №5:e20009. – doi:10.1371/journal.pone.0020009.
22. Reliability of biopsy-based diagnostic tests for Helicobacter pylori after treatment aimed at its eradication / E.J. van der Wouden, J.C. Thijs, A. A. van Zwet [et al.] // Eur J Gastroenterol Hepatol. – 1999. – Vol. 11. – P. 1255–1258.
23. Schistosomes, liver flukes and Helicobacter pylori: views and expert opinions of an IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Lyon, France. – 1994.
Review
For citations:
Gotovtsev N.N., Barashkov N.A., Borisova Т.V., Pak M.V., Alekseeva M.P., Innokentieva N.N., Loskutova K.S., Pshennikova V.G., Rafailov A.M., Lekhanova S.N., Fedorova S.A. Approbation of the molecular genetic method for the diagnosis of Helicobacter pylori infection in Yakutia. Yakut Medical Journal. 2017;(3):12-15.
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