Nad-dependent deacetylase genes of sirtuin family and risk of developing various phenotypes of chronic obstructive pulmonary disease
G. F. Korytina,
V. A. Markelov,
L. Z. Akhmadishina,
Yu. G. Aznabaeva,
O. V. Kochetova,
A. P. Larkina,
N. N. Khusnutdinova,
S. M. Izmailova,
N. Sh. Zagidullin,
T. V. Viktorova https://doi.org/10.25789/YMJ.2023.83.03
Abstract
Chronic obstructive pulmonary disease (COPD) is one of the most common chronic respiratory diseases with high morbidity and mortality. The pathogenesis of COPD is closely related to oxidative stress, that is the main mechanism causes accelerated cell senescence. Published data suggest that the COPD pathogenesis may involve stress responses dysregulation that inhibit cellular senescence. We aimed to assess the contribution of sirtuin genes (SIRT2, SIRT1, SIRT3, SIRT6) to the various COPD phenotypes risk.
SNPs of SIRT2 (rs10410544), SIRT1 (rs3758391, rs3818292), SIRT3 (rs3782116, rs536715), SIRT6 (rs107251) genes were genotyped by the real-time polymerase chain reaction (PCR) among 1245 samples (severe COPD with frequent exacerbations (N=331), stable COPD with rare exacerbations (N=290) and control (N=624)). Logistic regression was used to detect the association of studied SNPs in different models.
Significant associations with severe COPD phenotype were identified for SIRT1 (rs3818292) (P=0.0097, OR = 1.49 for AG genotype), SIRT3 (rs3782116) (P = 0.0034, OR =0.63) and SIRT3 (rs536715) (P = 0.00001, OR =0.53) under dominant model, and SIRT6 (rs107251) (P = 0.00001, OR =0.55 for СT genotype). Stable COPD phenotype with rare exacerbations was associated with SIRT1 (rs3818292) (P = 0.0055, OR =1.54 for AG genotype), SIRT3 (rs536715) (P = 0.00001, OR =0.48 under dominant model), and SIRT6 (rs107251) (P = 0.0002, OR =0.54 for СT genotype).
The obtained results indicate the contribution of NAD-dependent deacetylase genes of sirtuin family and cellular senescence mechanisms to COPD development. The SIRT3 (rs3782116) identified as a specific marker for severe COPD phenotype with frequent exacerbations.
About the Authors
G. F. Korytina
Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of ciences (IBG UFRC)
Russian Federation
Russian Federation
KORYTINA Gulnaz Faritovna – MD in Biology, Associate Professor, Head of Scientific Research, Prof. Bashkir State Medical University of the Ministry of Health of Russia
Ufa
V. A. Markelov
Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of ciences (IBG UFRC)
Russian Federation
Russian Federation
MARKELOV Vitaly Andreevich – full-time postgraduate student, junior researcher of the Bashkir State Medical University of the Ministry of Health of Russia
Ufa
L. Z. Akhmadishina
Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of ciences (IBG UFRC)
Russian Federation
Russian Federation
AKHMADISHINA Leysan Zinurovna – PhD in Biology, research asocciate
Ufa
Yu. G. Aznabaeva
Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of ciences (IBG UFRC)
Russian Federation
Russian Federation
AZNABAYEVA Yulia Gennadievna – Candidate of Medical Sciences, Associate Professor, Head Teacher of the Bashkir State Medical University of the Ministry of Health of Russia
Ufa
O. V. Kochetova
Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of ciences (IBG UFRC)
Russian Federation
Russian Federation
KOCHETOVA Olga Vladimirovna – PhD in Biology, Senior Researcher, IBG UFRC RAS
Ufa
A. P. Larkina
Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of ciences (IBG UFRC)
Russian Federation
Russian Federation
LARKINA Anastasia Pavlovna – junior researcher, IBG UFRC RAS
Ufa
N. N. Khusnutdinova
Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of ciences (IBG UFRC)
Russian Federation
Russian Federation
KHUSNUTDINOVA Natalia Nikolaevna – PhD in Biology, research associate IBG UFRC RAS
Ufa
S. M. Izmailova
Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of ciences (IBG UFRC)
Russian Federation
Russian Federation
ZMAILOVA Svetlana Mikhailovna – PhD in Biology, Associate Professor of Bashkir State Medical University of the Ministry of Health of Russia
Ufa
N. Sh. Zagidullin
Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of ciences (IBG UFRC)
Russian Federation
Russian Federation
ZAGIDULLIN Naufal Shamilevich – MD, Professor, Head of the Department of Bashkir State Medical University of the Ministry of Health of Russia
Ufa
T. V. Viktorova
Institute of Biochemistry and Genetics - Subdivision of the Ufa Federal Research Centre of the Russian Academy of ciences (IBG UFRC)
Russian Federation
Russian Federation
VIKTOROVA Tatiana Viktorovna – MD, Prof., Head of the Department of Bashkir State Medical University of the Ministry of Health of Russia
Ufa
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Review
For citations:
Korytina G.F., Markelov V.A., Akhmadishina L.Z., Aznabaeva Yu.G., Kochetova O.V., Larkina A.P., Khusnutdinova N.N., Izmailova S.M., Zagidullin N.Sh., Viktorova T.V. Nad-dependent deacetylase genes of sirtuin family and risk of developing various phenotypes of chronic obstructive pulmonary disease. Yakut Medical Journal. 2023;(3):14-17. https://doi.org/10.25789/YMJ.2023.83.03
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