Study of XAV-939 effect on cancer stem cells of human colorectal cancer in heterotopic models in vivo
https://doi.org/10.25789/YMJ.2023.83.01
Abstract
The purpose of this study was to investigate the numbers of cells with CSC markers in samples of human colorectal cancer (CRC) xenografts isolated in animals with tumors after exposure to the small molecule XAV-939, 5-fluorouracil, and to the combination of XAV-939 with 5-fluorouracil. The model was created in Balb/c Nude mice. Tumor fragments of human CRC were transplanted into adipose tissue of animals under the skin of the right thigh. The fourth xenograft generation was used for the study. Animals were divided into groups receiving XAV-939, 5-fluorouricil, their combination, and the control group. Tumor nodes were measured twice a week; at the end of the experiment, the number of cells with the CSC phenotype was determined in the tumor material of the animals. By the end of the experiment, tumor nodules in the group of animals receiving the combination of drugs were significantly smaller than in the control group. The highest levels of cells with phenotypic signs of CSCs were characteristic of samples from the control group, and for samples obtained from the group receiving 5-fluorouracil as monotherapy. Decreased numbers of these cells were observed in groups receiving XAV-939 and the combination of XAV-939 and 5-fluorouracil.
About the Authors
A. S. Goncharova
National Medical Research Centre for Oncology
Russian Federation
Russian Federation
GONCHAROVA Anna Sergeevna – PhD in Biology, Head of Experimental Laboratory Center
Rostov-on-Don
G. Yu. Egorov
National Medical Research Centre for Oncology
Russian Federation
Russian Federation
EGOROV Georgiy Yurievich – postgraduate student
Rostov-on-Don
E. N. Kolesnikov
National Medical Research Centre for Oncology
Russian Federation
Russian Federation
KOLESNIKOV Evgeniy Nikolaevich – MD
Rostov-on-Don
A. Yu. Maksimov
National Medical Research Centre for Oncology
Russian Federation
Russian Federation
MAKSIMOV Aleksey Yurievich – MD, Deputy General Director for Prospective Scientific Research
Rostov-on-Don
M. V. Romanova
National Medical Research Centre for Oncology
Russian Federation
Russian Federation
ROMANOVA Maria Vadimovna – junior researcher
Rostov-on-Don
A. B. Sagakyants
National Medical Research Centre for Oncology
Russian Federation
Russian Federation
SAGAKYANTS Aleksandr Borisovich – Head of Laboratory of Tumor Immunophenotypin
Rostov-on-Don
E. S. Bondarenko
National Medical Research Centre for Oncology
Russian Federation
Russian Federation
BONDARENKO Elena Sergeevna – junior researcher
Rostov-on-Don
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Review
For citations:
Goncharova A.S., Egorov G.Yu., Kolesnikov E.N., Maksimov A.Yu., Romanova M.V., Sagakyants A.B., Bondarenko E.S. Study of XAV-939 effect on cancer stem cells of human colorectal cancer in heterotopic models in vivo. Yakut Medical Journal. 2023;(3):5-8. https://doi.org/10.25789/YMJ.2023.83.01
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