Modeling of liver hypoxia in an in vivo experiment

   The effect of portal triad blood flow reduction on liver enzyme levels, liver condition, and survival in laboratory mice was studied. Alanine aminotransferase and aspartate aminotransferase levels in both blood flow reduction groups were statistically significantly higher than in the control group, and statistically significantly higher in the blood sampling group 24 hours after reperfusion compared to the blood sampling group 2 hours after reperfusion. Histological examination showed signs of ischemic tissue damage, and an increase in the number of vessels in liver preparations from animals with blood flow reduction compared to the control group was observed. The survival rate of animals after the procedure was more than 80 %, which is satisfactory, but nevertheless indicates the need for a number of animals that will allow statistical processing of the results even in the event of death of some individuals. The results of the study showed that the obtained model is an important tool for research in the field of studying pathological conditions of the liver associated with ischemic and hypoxic conditions.

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