Way of direct definition of medicinal sensitivity of tuberculosis mycobacteria to antitubercular preparations

Abstract

We report the results of modifi ed direct method of M.tuberculosis drug susceptibility testing in 70 patients with newly identifi ed moderate to heavy bacterioexcretion using a microscopic technique. Test techniques under assessment were the described direct method and indirect absolute concentration method. Agreement in 100% of cases was found in test results of susceptible strains. In resistant strains, agreement between compared test techniques was 89.3%, 88.9%, 90.5%, and 81.8% for strains resistant to streptomycin, isoniazid, rifampycin and kanamycin, respectfully. Furthermore, high agreement rate of test results (94.1%) was observed in multidrug-resistant strains, while the time of drug susceptibility assessment reduced to 22-24 days. The described method is low-cost and is available for all kinds of laboratories. The method received a patent of Russian Federation (no. 2244927).