The role of lle105Val polymorphism of the gstp1 gene in the development of allergic pathology in the children population of the industrial center of Western Ural
https://doi.org/10.25789/YMJ.2024.86.12
Abstract
A modern approach to the diagnostic support of patients with allergic diseases involves the introduction of innovative developments in the field of precision medicine, the development, identification and use of reliable biomarkers, including the determination of individual genetic variability in living conditions at various territories of the Russian Federation. The aim of the study is to investigate the role of the Ile105Val polymorphism of the GSTP1 gene (rs1695) in the development of allergic pathology and its connection with the characteristics of the formation of the immune profile in the child population of the industrial center of the Western Urals. Materials and methods. The observation group consisted of 34 children with allergic pathology. The comparison group included 37 children, relatively healthy, without allergies. Markers of hypersensitivity and cytokine status were studied using enzyme-linked immunosorbent assay. Genotyping was performed using real-time polymerase chain reaction. Results. In the group of children with allergic pathology, an increase in the eosinophilic-lymphocyte index was shown by 1.8 times, total IgE concentration by 4.7 times, a change in the level of serum cytokines IL-10 by 1.3 times and IL-4 by 1.8 times relative to the comparison group (p=0.005-0.038). Genetic analysis of the Ile105Val polymorphism of the GSTP1 gene revealed a 2.0-fold increase in the frequency of the homozygous AA genotype in the observation group (p=0.01). Allele A was associated with the development of allergies in the examined children (OR=2.36; 95% CI=1.16-4.79), while allele G had a protective value (OR=0.33; 95% CI= 0.12-0.89). An increase in the concentration of total IgE and blood eosinophils in carriers of the AA genotype was shown to be 4.6 times and 1.9 times, respectively, relative to owners of the AG and GG genotypes (p=0.013-0.031). Conclusion. Allele A and genotype AA of the polymorphic variant Ile105Val of the GSTP1 gene in children with allergic pathology act as sensitivity markers associated with the formation of allergies, a significant increase in total IgE, blood eosinophils and the anti-inflammatory cytokine IL-4, and can be considered as promising indicators of pathophysiological conditions associated with risk of development of atopic processes (allergic pathology) in children (RR=1.61; 95% CI=1.06-2.44) living in the Western Urals.
About the Authors
K. G. Starkova
FBSI Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”
Russian Federation
Russian Federation
Starkova Ksenia Gennadievna – Head of the Laboratory of Immunology and Allergology, PhD in Biology
O. V. Dolgikh
FBSI Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”
Russian Federation
Russian Federation
Dolgikh Oleg Vladimirovich – Head of the Department of Immunobiological Diagnostic Methods
V. B. Alekseev
FBSI Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”
Russian Federation
Russian Federation
Alekseev Vadim Borisovich – Director.
O. A. Kazakova
FBSI Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”
Russian Federation
Russian Federation
Kazakova Olga Alekseevna – Head of the Laboratory of Immunogenetics
T. A. Legostaeva
FBSI Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”
Russian Federation
Russian Federation
Legostaeva Tatyana Andreevna – doctor of clinical laboratory diagnostics
References
1. Amromina A.M., Sitnikov I.A., Shaikhova D.R. The relationship of polymorphic variants of genes GSTM1, GSTT1, GSTP1 with the risk of developing diseases (literature review)] // Hygiene and Sanitation. 2021. No 100(12). P. 1385-1390. https://doi.org/10.47470/0016-9900-2021-100-12-1385-1390
2. Molecular and genetic aspects of health risks and their association with adverse environmental conditions and diets (systemic review)] / Mazhaeva T.V., et al. // Health Risk Analysis. 2022. No 4. P.186-197.https://doi.org/10.21668/health.risk/2022.4.18
3. Polymorphisms of xenobiotic biotransformation genes of the glutathione-S-transferase family (GTSs). Review] / Knizhnikova E.V., et al. // Bulletin Physiology and Pathology of Respiration. 2020. No 75. P. 115-125. http://dx.doi.org/10.36604/1998-5029-2020-75-115-125
4. Simbirtsev A.S.Cytokines and their role in immune pathogenesis of allergy] // Russian Medical Inquiry. 2021. No 5(1). P. 32-37. http://dx.doi.org/10.32364/2587-6821-2021-5-1-32-37
5. Advances and highlights in biomarkers of allergic diseases / Ogulur I., et al. // Allergy. 2021. Vol. 76(12). P. 3659-3686. http://dx.doi.org/doi:10.1111/all.15089
6. Asia Pacific Association of Allergy Asthma and Clinical Immunology White Paper 2020 on climate change, air pollution and biodiversity in Asia-Pacific and impact on allergic diseases / Pawankar R. [et al.] // Asia Pac. Allergy. 2020. Vol. 10(1): e11. https://doi.org/10.5415/apallergy.2020.10.e11
7. Basharat Z., Yasmin A. Energy landscape of a GSTP1 polymorph linked with cytological function decay in response to chemical stressors // Gene. 2017. Vol. 609. P. 19-27. https://doi.org/10.1016/j.gene.2017.01.034
8. Biomarkers for diagnosis and prediction of therapy responses in allergic diseases and asthma / Breiteneder H. [et al.] // Allergy. 2020. Vol. 75(12). P. 3039-3068. https://doi.org/10.1111/all.14582
9. Dai X., Dharmage S.C., Lodge C.J. Interactions between glutathione S-transferase genes and household air pollution on asthma and lung function // Front. Mol. Biosci. 2022. Vol. 9: 955193. https://doi.org/10.3389/fmolb.2022.955193
10. Do glutathione S-transferase genes modify the link between indoor air pollution and asthma, allergies, and lung function? A systematic review / Dai X. [et al.] // Curr. Allergy Asthma Rep. 2018. Vol. 18: 20. https://doi.org/10.1007/s11882-018-0771-0
11. Do variants in GSTs modify the association between traffic air pollution and asthma in adolescence? / Bowatte G. [et al.] // Int. J. Mol. Sci. 2016. Vol. 17(4): 485. https://doi.org/10.3390/ijms17040485
12. Interactions of GST polymorphisms in air pollution exposure and respiratory diseases and allergies / Bowatte G. [et al.] // Curr. Allergy Asthma Rep. 2016. Vol. 16(12): 85. https://doi.org/10.1007/s11882-016-0664-z
13. Kelchtermans J., Hakonarson H. The role of gene-ambient air pollution interactions in paediatric asthma // Eur. Respir. Rev. 2022. Vol. 31: 220094 https://doi.org/10.1183/16000617.0094-2022.
14. Oxidative stress is associated with atopic indices in relation to childhood rhinitis and asthma / Wei Choo C. Y. [et al.] // J. Microbiol. Immunol. Infect. 2021. Vol. 54(3). P. 466-473. https://doi.org/10.1016/j.jmii.2020.01.009
15. Takano H., Inoue K.I. Environmental pollution and allergies // J. Toxicol. Pathol. 2017. Vol. 30(3). P. 193-199. https://doi.org/10.1293/tox.2017-0028.
16. The multifaceted role of glutathione S-transferases in health and disease / Mazari A.M.A., et al. // Biomolecules. 2023. Vol. 13(4): 688. https://doi.org/10.3390/biom13040688.
17. The relevance analysis of GSTP1 rs1695 and lung cancer in the Chinese Han population / Xiao J. [et al.] // Int. J. Biol. Markers. 2021. Vol. 36(3). P. 48-54. https://doi.org/10.1177/17246008211039236.
18. Zarth A.T., Murphy S.E., Hecht S.S. Benzene oxide is a substrate for glutathione S-transferases // Chem. Biol. Interact. 2015. Vol. 242. P. 390-395. https://doi.org/10.1016/j.cbi.2015.11.005.
Review
For citations:
Starkova K.G., Dolgikh O.V., Alekseev V.B., Kazakova O.A., Legostaeva T.A. The role of lle105Val polymorphism of the gstp1 gene in the development of allergic pathology in the children population of the industrial center of Western Ural. Yakut Medical Journal. 2024;(2):50-53. https://doi.org/10.25789/YMJ.2024.86.12
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