Polymorphism of the microsomal epoxide hydrolase EPHX1 gene (rs1051740) in often ill children living
https://doi.org/10.25789/YMJ.2023.83.16
Abstract
The aim of the study was to analyze frequency of the EPHX1 gene polymorphism (rs1051740) associated with diseases of the upper airways and elevated manganese levels in biological media of children from an industrial area located in the Southern Siberia.
Materials and methods. We examined children aged 4-7 years who permanently lived in an industrial area in the Southern Siberia. It was a monotown with its economy dominated by a large non-ferrous metallurgy plant. The test group was made of 60 children who were often sick (more than 6 times a year) for a long time. The reference group included 39 conditionally healthy children with manganese levels in their blood being within the reference range. We identified frequency of polymorphism of the microsomal epoxide hydrolase EPHX1 gene (rs1051740) and the cytochrome C level using PCR and ELISA accordingly. The statistical significance was taken at p
Results and discussion. Average manganese levels were 1,8 times significantly higher in blood of the children from the test group than in the reference one. We established statistically significant authentic differences in frequencies of the EPHX1 gene genotypes and alleles (rs1051740) between the test and reference groups (the C/C genotype was 3,2 times more frequent; the С allele, 1,5 times, p
Conclusions. The study established several peculiarities in children who often had diseases of the upper air ways (the test group). They had elevated manganese levels in their blood, higher than its safe level; the serum cytochrome C level was lower in them; they more frequently had the C/C genotype (OR=4,05, 95% CI=1,26-13,05) and the С allele (OR=1,98, 95% CI=1,09-3,60) of the EPHX1 gene (rs1051740). Many authors believe polymorphism of this gene to be a risk factor able to cause respiratory diseases. Deficiency of microsomal epoxide hydrolase 1, combined with the candidate gene polymorphism, disrupts detoxification, promotes accumulation of non-conjugated chemical exogenous factors (manganese), inhibits anti-oxidation and weakens the immunity. All this makes children who live in the analyzed town fall sick with respiratory diseases more frequently.
About the Authors
N. V. Zaitseva
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Russian Federation
Russian Federation
ZAITSEVA Nina Vladimirovna – MD, Scientific Director
O. V. Dolgikh
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Russian Federation
Russian Federation
DOLGIKH Oleg Vladimirovich – MD, head of the Department of Immunobiological Diagnostics
A. A. Subbotina
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Russian Federation
Russian Federation
SUBBOTINA Alena Aleksandrovna – junior researcher at the Immunogenetics laboratory of the Department of Immunobiological Diagnostics
A. V. Yaroma
Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Russian Federation
Russian Federation
YAROMA Alesya Vyacheslavovna – junior researcher at the Cellular Diagnostic Laboratory
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Review
For citations:
Zaitseva N.V., Dolgikh O.V., Subbotina A.A., Yaroma A.V. Polymorphism of the microsomal epoxide hydrolase EPHX1 gene (rs1051740) in often ill children living. Yakut Medical Journal. 2023;(3):65-67. https://doi.org/10.25789/YMJ.2023.83.16
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