Practical application of the immunocytochemical research method in the diagnosis of precancerous diseases of the cervix
https://doi.org/10.25789/YMJ.2023.83.12
Abstract
Patients with cervical pathology were analyzed by cytological, immunocytochemical and molecular genetic tests. Among the selected groups, 78 women were more likely to have low-grade dysplasia, or LSIL (16.3%). In more than half of the cases (60.2%) in women who were tested, positive HPV tests were observed. The majority of positive HPV tests in the were for high oncogenic risk (32%), with 16 (19.1%) and 31 (8.5%) being the most common types. It was found that the frequency of double staining of p16ink4a/Ki67 was noted in women with a high degree of intraepithelial lesion (37%), and the detection of HPV type 16 was prevailed - 19.1%. The number of cases in the group of women without pathology, but with latent HPV infection, cases with a positive HPV test and a negative result of p16/Ki67 protein expression prevailed was 94.1%. It was found that the number of p16-positive cells was higher in women of group 1 with LSIL (55.8%) and group 2 (44.4%) with HSIL. In the group of women without cervical pathology, but with latent HPV infection, indicators of weak expression of p16ink4a were most frequently recorded (57.1%).
About the Authors
M. P. Kirillina
Yakut Science Centre of Complex Medical Problems
Russian Federation
Russian Federation
KIRILLINA Maria Petrovna – PhD in Biology, senior researcher, Head of the laboratory of precancerogenesis and malignant tumors of the Yakut Science Centre of Complex Medical Problems, Head of NEFU Medical Institute Clinic Laboratory
S. I. Sofronova
Yakut Science Centre of Complex Medical Problems
Russian Federation
Russian Federation
SOFRONOVA Sargylana Ivanovna – PhD, chief researcher, head of the science division
V. M. Nikolaev
Yakut Science Centre of Complex Medical Problems
Russian Federation
Russian Federation
NIKOLAEV Vyacheslav Mikhailovich – PhD in Biology, senior researcher of the laboratory of precancerogenesis and malignant tumors
A. S. Golderova
M. K. Ammosov NEFU
Russian Federation
Russian Federation
GOLDEROVA Aytalina Semenovna – MD, Professor of the department of Public Health and Health Service, Common Hygiene and Bioethics, Head of Research Laboratory (NIL) of cell technology and regenerative medicine
K. V. Komzin
M. K. Ammosov NEFU Medical Institute
Russian Federation
Russian Federation
KOMZIN Kirill Vasilievich – senior lector of the Department of Histology and Microbiology
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Review
For citations:
Kirillina M.P., Sofronova S.I., Nikolaev V.M., Golderova A.S., Komzin K.V. Practical application of the immunocytochemical research method in the diagnosis of precancerous diseases of the cervix. Yakut Medical Journal. 2023;(3):47-51. https://doi.org/10.25789/YMJ.2023.83.12
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