Comparative analysis of Mycobacterium tuberculosis genotypes in the Republic Sakha (Yakutia) and the Irkutsk region
Abstract
Multidrug-resistant tuberculosis without prior treatment (primary MDR-TB) is a major problem in the Russian Federation, and also in Eastern Siberia and Far East. Mycobacterium tuberculosis isolates from patients with primary TB at two referral sites in Irkutsk oblast and the Sakha Republic (Yakutia) were tested for conventional susceptibility and genotyped using MIRU-12. Outcomes from primary MDR-TB were compared between sites. Phylogenetic reconstruction was used to determined basic epidemic genotypes with MDR. Primary MDR-TB was common in both regions and phylogenetic patterns; drug-resistance and outcomes carried regionally distinct differences, emphasizing the need for locally informed diagnosis and treatment strategies.
About the Authors
S. N. Zhdanova
Scientific Center for Family Health and Human Reproduction Problems, Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
Zhdanova Svetlana Nikolaevna – Cand.Med.Sc., Senior Researcher
Irkutsk
G. I. Alexeeva
Research Practice Center for Phthisiatry
Russian Federation
Russian Federation
Alexeeva Galina Ivanovna – Dr.Med.Sc., Head of Bacteriological Laboratory
Yakutsk, Sakha Republic (Yakutia)
O. B. Ogarkov
Scientific Center for Family Health and Human Reproduction Problems, Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
Ogarkov Oleg Borisovich – Cand.Biol.Sc., Laboratory Head; Irkutsk Regional TBPrevention Dispensary
Irkutsk
A. F. Kravchenko
Research Practice Center for Phthisiatry
Russian Federation
Russian Federation
Kravchenko Alexander Fedorovich – Dr.Med.Sc., Director
Yakutsk, Sakha Republic (Yakutia)
E. Yu. Zorkaltseva
Irkutsk State Medical Continuing Education Academy
Russian Federation
Russian Federation
Zorkaltseva Elena Yulievna – Dr.Med.Sc., Department Chair
M. K. Vinokurova
Research Practice Center for Phthisiatry
Russian Federation
Russian Federation
Vinokurova Maria Konstantinovna – Dr.Med.Sc., Deputy Director for Science
Yakutsk, Sakha Republic (Yakutia)
E. D. Savilov
Irkutsk State Medical Continuing Education Academy
Russian Federation
Russian Federation
Savilov Eugeniy Dmitrievich – Dr.Med.Sc., Professor, Department Chair
References
1. Molecular typing of Mycobacterium tuberculosis strains in Irkutsk (East Siberia) in 2000-2005/ O.B. Ogarkov, T.V. Medvedeva, T. Zozio [ et al.] // Mol Meditsina.- 2007.-№2.-P.33–38.
2. Characterization of multidrug-resistant Mycobacterium tuberculosis strains by molecular genetic methods / S.N. Zhdanova, E.Yu. Zorkaltseva, O.B. Ogarkov, O.A. Vorobieva [et al.] // Sibirskiy meditsinskiy zhurnal (Irkutsk).- 2011.- №6.-P.228-230.
3. Epidemiologic analysis: Statistical data processing methods / E.D. Savilov, V.A. Asrafiev, S.N. Zhdanova, E.A. Zarudnev.- Novosibirsk: “Nauka-Tsentr”, 2011.- 156 p.
4. A publicly available international multimarker database for studying Mycobacterium tuberculosis genetic diversity and molecular epidemiology / C. Demay, B. Liens, T. Burguière, V. Hill [et al.] // SITVITWEB. Infect Genet Evol. - 2012. - 12(4). - P. 755-66.
5. Automated high-throughput genotyping for study of global epidemiology of Mycobacterium tuberculosis based on mycobacterial interspersed repetitive units / Supply P., Lesjean S., Savine E. [et al.] // J Clin Microbiol. - 2001. - 39(10). - P. 3563-71.
6. Central Research Institute of Health Organization and Information. Key indicators of tuberculosis in Russia in 2009. Accessed at http://tbpolicy.ru/statistic/national/ April, 2012.World Health Organization. Global tuberculosis control report. World Health Organization, Geneva, Switzerland, 2011.
7. Characterization of drug-resistant isolates of Mycobacterium tuberculosis derived from Russian inmates / I.G. Shemyakin, V.N. Stepanshina, I.Y. Stepanshina [et al.] // Int J Tuberc Lung Dis. - 2004. - 8(10). - P. 1194–1203.
8. Dispersal of Mycobacterium tuberculosis via the Canadian fur trade / C.S. Pepperell, J.M. Granka, D.C. Alexander [et al.] // Proc Nat Acad Sci. - 2011. - 108(16). P. 6526–6531.
9. Hamers F.F., Downs A.M. HIV in central and eastern Europe / F.F. Hamers, A.M. Downs // Lancet 2003; 361: 1035–44.
10. Highest prevalence of Mycobacterium Beijing genotype isolates in patients newly diagnosed with tuberculosis in the Novosibirsk oblast, Russian Federation / M.A. Dymova, V.N. Kinsht, A.G. Cherednichenko [et al.] // J Med Microbiol. - 2011. - 60. - P. 1003-09.
11. Irkutsk Oblast Center for AIDS and Infectious Diseases Prevention and Control. Accessed from Russian Red Cross 2011 report, http://www.redcross-irkutsk.org/en/news. June, 2012.
12. Major Mycobacterium tuberculosis lineages associate with patient country of origin / M.B. Reed, V.K. Pichler, F. McIntosh [et al.] // J Clin Microbiol. - 2009. - 47(4). - P. 1119-28.
13. Mokrousov I. Genetic geography of Mycobacterium tuberculosis Beijing genotype: a multifacet mirror of human history? / I. Mokrousov // Infect Genet Evol. - 2008. - 8 (6). - P. 777–785.
14. Molecular characterization of multiple drug-resistant Mycobacterium tuberculosis isolates from northwestern Russia and analysis of rifampin resistance using RNA/RNA mismatch analysis as compared to line probe assay and sequencing of rpoB gene. / I.Mokrousov, I. Filliol, E.Legrand [et al.] // Res Microbiol. - 2002. - 153. - P. 213-219.
15. Molecular typing of Mycobacterium tuberculosis circulated in Moscow, Russian Federation / M.V. Afanas’ev, L.N. Ikryannikova, E.N. Il’na [et al.] // Eur J Clin Microbiol Infect Dis. - 2011. - 30. - P. 181–191.
16. Mycobacterium tuberculosis Beijing genotype in Russia: in search of informative variable-number tandem-repeat loci / I.Mokrousov, O. Nrvskaya, A.Vyazovaya [et al.] // J Clin Microbiol. - 2008. - 46. - P. 3576-3584.
17. Rifampin- and multidrug-resistant tuberculosis in Russian civilians and prison inmates: dominance of the Beijing strain family / F. Drobniewski, Y. Balabanova, M. Ruddy [et al.] // Emerg Infect Dis. - 2002. - 8. - P. 1320–1326.
18. Somerville W. Extraction of Mycobacterium tuberculosis DNA: a question of containment / W. Somerville, L. Thibert, K. Schwartzman, M.A. Behr // J Clin Microbiol. - 2005. - 43. - P. 2996–2997.
19. Variable host-pathogen compatibility in Mycobacterium tuberculosis / S. Gagneux, K. DeRiemer, T. Van [et al.] // Proc Natl Acad Sci USA. - 2006. - 103. - P. 2869–2873.
20. Weniger T. MIRU-VNTRplus: a web tool for polyphasic genotyping of Mycobacterium tuberculosis complex bacteria / T. Weniger, J. Krawczyk, P. Supply, S. Niemann, D. Harmsen // Nucleic Acids Res. - 2010. - 38. Suppl: W326-331.
21. World Health Organization. Laboratory services in tuberculosis control. Part III: culture. World Health Organization, Geneva, Switzerland, 1998.
22. World Health Organization. Multidrug and extensively drug-resistant TB (M/XDR-TB): 2010 global report on surveillance and response.- World Health Organization, Geneva, Switzerland, 2010.
23. World Health Organization. Global tuberculosis control report. -World Health Organization. Geneva, Switzerland, 2011.
24. 2010 All-Russia Population Census. Federal State Statistics Service. Accessed at http://www.gks.ru/free_doc/new_site/perepis2010/perepis_itogi1612.htm. April, 2012.
Review
For citations:
Zhdanova S.N., Alexeeva G.I., Ogarkov O.B., Kravchenko A.F., Zorkaltseva E.Yu., Vinokurova M.K., Savilov E.D. Comparative analysis of Mycobacterium tuberculosis genotypes in the Republic Sakha (Yakutia) and the Irkutsk region. Yakut Medical Journal. 2013;(1):68-71.
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