The use of «DNA-comet» method for the detection and assessment of damage to the blood mononuclear cells induced by endogenous intoxication at the acute destructive pancreatitis
Abstract
The method of analysis of DNA damage («DNA comet», DNA-comet assay, method of gel electrophoresis of individual cells DNA) found its application in clinical practice as a method of monitoring the effectiveness of treatment and the severity of genetoxicologic effect in the development of endogenous intoxication. The literature review data suggest a possible clinical application of the method in determining the level of endogenous intoxication in patients with acute destructive pancreatitis.
About the Authors
M. M. Vinokurov
medical institute of NEFU
Russian Federation
Russian Federation
Vinokurov Mikhail Mikhajlovich, doctor of medical sciences, professor of department of faculty surgery
677000, Street of Dzerjinskogo 26 flat 41. Office telephone (43-20-91), home telephone (45-25-30), Yakutsk
V. V. Savelev
medical institute of NEFU
Russian Federation
Russian Federation
Savelev Vyacheslav Vasilievich, candidate of medical sciences, associate professor of department of faculty surgery
677000 street of Krupskoj 37 flat 58. Mobile telephone 89241706330. Yakutsk
T. V. Yalinskaya
medical institute of NEFU
Russian Federation
Russian Federation
Yalinskaya Tatyana Vadimovna, student of the 5th course
677009 street of Dzerjinskogo 45/2 flat 70. Mobile telephone 89143033999
References
1. Influence of ecological imbalance in the population of the North Health on the example of the Republic Sakha (Yakutia ) / P.G. Petrova, B.M. Kerdhengolts, O.N. Kolosova, I.M. Meltzer // Far East Medical Journal. – 2001. №4. – P. 5-10.
2. The intensity of the processes of peroxidation and antioxidant defense in patients with infected pancreatic necrosis forms / M.M. Vinokurov, V.V. Savelev, E.S. Hlebnii, B.M. Kerdhengolts // Far East Medical Journal. – 2010. - №2. – P. 19.
3. Ivanov A.I. Complex treatment of suppurative complications in abdominal surgery / A.I. Ivanov, A.S. Grigor’ev, V.A. Petrova // Actual problems of public health in the Republic Sakha (Yakutia): mat. of the rep. scientific-practical conf. - Yakutsk, 1997. - P. 74-75.
4. Krivoshapkin V.G. Sketches of internal medicine in the North / V.G. Krivoshapkin. - Yakutsk, 2001. – 128 p.
5. Methodical aspects of assessment of DNA damage by DNA - comet method / A.K. Gantaev, V.A. Nikitina, E.S. Voronina, A.D. Durnev // Applied toxicology. - V.2. - №2 (4). – 2011. - p.28-37.
6. Mironova E.G. Status of antioxidant protection in the development of chronic obstructive bronchitis and the use of antioxidants in the treatment of patients in the Far North: dissertation of the doctor of biological sciences/ G.E. Mironova. – M., 2000. – 50 p.
7. Potapov A.F. Comprehensive assessment of intensive care surgical abdominal infections in a specialized Center for Emergency Medical Aid of the Republic Sakha (Yakutia): dissertation of the doctor of medical sciences / A.F. Potapov. – M., 2004. – 237 p.
8. Application of the alkaline gel electrophoresis to assess isolated cells genotoxic properties of natural and synthetic compounds: guidelines / Durnev A.D., Gantaev A.K., Anisina E.A. [et al.]. - M., 2006. - 28 p.
9. Sorochinskaya U.B. Application of the comet assay to assess DNA damage caused by various environmental agents / U.B. Sorochinskaya, V.M. Mikhailenko //Oncology.- P.10. - №3. – 2008. - p. 303-309.
10. Tyaptiganova T.M. Preoperative diagnosis of obstructive jaundice / T.M. Tyaptiganova, V.G. Ignatev // Materials of the Republican scientificpractical conference «Actual problems of emergency medical care. – Yakutsk, 2000. – № 1. – P. 49-51.
11. Environmental and human adaptation immunological problems in the North/ P.G. Petrova, O.N. Ivanova, F.A. Zaharov, V.V. Vadileva// Materials of the Republican scientificpractical conference «Achievements and perspectives of medical science in the Republic of Sakha (Yakutia ) «. – Yakutsk. – 2001. – p. 135-137.
12. Filippov E.V. The use of the «comet assay» to detect and assess the extent of DNA damage cells of organisms of plants, animals and humans,caused by environmental factors // Science and education. - Yakutsk. - 2014. - №2. - P. 72-78.
13. Chaubey RC. Computerized image analysis software for the comet assay / RC. Chaubey // Methods Mol Biol 2005; 291: 97–106.
14. Francesconi A. Standardization of the comet assay technique on FRTL5 cells /A. Francesconi, E. Del Terra, A. Meli, F.S. AmbesiImpiombato // Phys Med 2001; 17(1): 232–4.
Review
For citations:
Vinokurov M.M., Savelev V.V., Yalinskaya T.V. The use of «DNA-comet» method for the detection and assessment of damage to the blood mononuclear cells induced by endogenous intoxication at the acute destructive pancreatitis. Yakut Medical Journal. 2016;(2):87-89.
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