Methylation Status of the Cell Cycle Control-Associated Genes in the Carotid Artery Tissue of Atherosclerotic patients
Abstract
Objective: We tested the hypothesis that the aberrant methylation of the promoters and first exons of CDKN2A (p16INK4a, p14ARF), CDKN2B (p15INK4b) and RB1 genes was associated with carotid atherosclerosis.
Methods and results: The DNA methylation status of these cell cycle control-associated genes was analysed in 120 samples of carotid arteries using two different techniques: methylationsensitive polymerase chain reaction (MS-PCR) and methylation-specific PCR (MSP-PCR). DNA methylation was not detected in advanced atherosclerotic plaques or nearby macroscopically intact tissues of the vascular wall from the same patients.
Conclusion: The methylation status of CDKN2A, CDKN2B and RB1 genes does not appear to be a marker of human carotid atherosclerosis.
About the Authors
M. S. Nazarenko
Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
Maria S. Nazarenko, Ph.D
10 Ushaika Embankment, Tomsk, 634050
A. V. Markov
Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
Anton V. Markov
10 Ushaika Embankment, Tomsk, 634050
I. A. Koroleva
Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
Iuliia A. Koroleva ccccccc
10 Ushaika Embankment, Tomsk, 634050
I. N. Lebedev
Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
Igor N. Lebedev, Ph.D.
10 Ushaika Embankment, Tomsk, 634050
A. A. Sleptcov
Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
Alexei A. Sleptcov
10 Ushaika Embankment, Tomsk, 634050
A. V. Frolov
Research Institute for Complex Problems of Cardiovascular Diseases, Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
Alexey V. Frolov, Ph.D.
6 Sosnovy Blvd, Kemerovo, 650002
O. L. Barbarash
Research Institute for Complex Problems of Cardiovascular Diseases, Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
Olga L. Barbarash, Ph.D., MD
6 Sosnovy Blvd, Kemerovo, 650002
V. P. Puzyrev
Research Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
Valery P. Puzyrev Ph.D., MD
10 Ushaika Embankment, Tomsk, 634050
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Review
For citations:
Nazarenko M.S., Markov A.V., Koroleva I.A., Lebedev I.N., Sleptcov A.A., Frolov A.V., Barbarash O.L., Puzyrev V.P. Methylation Status of the Cell Cycle Control-Associated Genes in the Carotid Artery Tissue of Atherosclerotic patients. Yakut Medical Journal. 2014;(2):61-63.
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