Intensity of lipid peroxidation in patients with cold injury
https://doi.org/10.25789/YMJ.2018.62.11
Abstract
The authors report their study on the intensity of lipid peroxidation processes and the state of antioxidant protection in patients with cold trauma of varying severity. It was established that in the organism of patients with frostbite of extremities, the intensity of free radical oxidation and the level of antioxidant defense depended on the degree of frostbite.
Clinical and biochemical studies were conducted in 151 people, 81 of whom were patients with a cold trauma of varying severity, who entered the burn department of the Republican Hospital №2. The control group included 70 practically healthy people. The material of the study was fasting venous blood samples, drawn from the ulnar vein.
The parameters of the intensity of free radical lipid oxidation (malonicdialdehyde, diene conjugates) and antioxidant protection (low-molecular antioxidants, activity of superoxide dismutase, catalase, glutathione peroxidase, glutathionereductase) were determined by spectrophotometric methods. Clinical and biochemical parameters in blood serum were determined on the biochemical automatic analyzer CobasMiraPlus (Roche).
In the body of patients with frostbite of the extremities of the first and second groups, a significant increase in the parameters of lipid peroxidation - MDA and DC - is observed. In patients of the second group, the intensity of lipid peroxidation processes was higher than in patients of the first group.
The state of the body’s antioxidant defense depends on the severity of the injury. In patients of the first group, the activity of SOD is increased by 8 times, CAT by 1.3 times, HP by 1.7 times, and GR activity decreased by 1.9 times in comparison with the control. The concentration of LMWA increases 2.1 times, and the content of ascorbic acid decreases by 1.2 times. In patients of the second group with respect to control, the activity of SOD increases 12.6 times, catalase 1.1 times, HP 1.5 times, and GH activity decreased 2.8 times. The content of LMAO increases 1.5 times, and the level of ascorbic acid in the blood decreases 2.4 times.
The shift in the prooxidant-antioxidant equilibrium toward the intensification of free radical reactions is also indicated by an increase in the coefficients: KSOD / CAT, KSOD / HP. The values of the coefficients (KSOD / CAT, KSOD / GP) depend on the severity of the cold trauma.
Keywords
Supporting agencies: The work was carried out with the financial support of the Ministry of Education and Science of the Russian Federation №6.1766.2017.ПЧ, the project of NEFU named after M.K. Ammosov: «Genetic features of the population of Yakutia: the structure of the gene pool, adaptation to cold, psychogenetic characteristics, the prevalence of some hereditary and infectious diseases» and the program of bioresource collections of the FANO «The Genome of Yakutia» YSC CMP (БРК: 0556-2017-0003).
About the Authors
V. M. Nikolaev
Yakutsk Scientific Center of Complex Medical Problems
Russian Federation
Russian Federation
Nikolaev Vyacheslav Mikhailovich - PhD, head of the department for studying adaptation mechanisms
677010, Yakutsk, ul. Sereylyakhskoyeshosse 4
phone: (4112) 32-19-81
R. Z. Alekseev
M.K. Ammosov North-Eastern Federal University; Yakutsk Scientific Center of Complex Medical Problems
Russian Federation
Russian Federation
Alekseev Revo Zakharovich - MD, Professor; Senior Researcher
677010, Yakutsk, ul. Sergelyakhskoyeshosse 4
phone: (4112) 32-19-81
S. A. Fedorova
M.K. AmmosovNorth-Eastern Federal University
Russian Federation
Russian Federation
Fedorova Sardana Arkadevna - Doctor of Biological Sciences, Head of the Research Laboratory of Molecular Biology
677000, Yakutsk, ul. Kulakovsky, 46, k.504
phone: (4112) 49-68-42
References
1. Vihriev B.S. Kichemasov S.Kh. Skvorcov Yu.R. Mestnye porazheniya holodom [Local cold lesions]. Leningrad, Medicina, 1991, p. 192.
2. Vladimirov Yu.A. Kalcievye nasosy zhivoj kletki [Calcium pumps of a living cell]. Sorosovskij obrazovatelnyj zhurnal [Sorosovsky educational journal]. Moscow, 1998, №3, p. 20 – 27.
3. Vladimirov Yu.A. Svobodnoradikalnoe okislenie lipidov i fizicheskie svojstva lipidnogo sloya biologicheskih membran [Free radical oxidation of lipids and physical properties of the lipid layer of biological membranes]. Biofizika [Biophysics]. Moscow, 1987, V.32, №5, P. 830-844.
4. Vladimirov Yu.A. Svobodnye radikaly v biologicheskih sistemah [Free radicals in biological systems] Sorosovskij obrazovatelnyj zhurnal [Sorosovsky educational journal]. Moscow, 2000, V.6, №12. - P. 13 - 19.
5. Voinov A.I. Otmorozheniya konechnostej [Frozen extremities]. Minsk: GIZAO Maladnyak, 1995, p. 141.
6. Garkavi L.Kh. Kvakina E.B. Kuzmenko T.S. Antistressornye reakcii i aktivacionnaya terapiya. Reakciya aktivacii kak put k zdorovyu cherez processy samoorganizacii [Anti-stress reactions and activation therapy. Reaction of activation as a path to health through self-organization processes]. Ekaterinburg, 2002, V. 1, p. 196.
7. Danilova L.A. Spravochnik po laboratornym metodam issledovaniya [Handbook of laboratory methods of research]. SPb., Piter, 2003, p. 398 - 399.
8. Klebanov G.I. Lyubickij O.B. Vasileva O.V. Antioksidantnye svojstva proizvodnyh 3-oksipiridina meksidola ehmoksipina i proksipina [Antioxidant properties of 3-hydroxypyridine derivatives: mexidol, emoxipin and proxipin] Voprosy medicinskoj himii [Questions of medical chemistry]. Moscow, 2001, V. 47, №3, p. 288 – 299.
9. Kolosova N.G. Shorin Yu.P. Kulikov V.Yu. Reakcii perekisnogo okisleniya lipidov v pecheni krys pri dolgovremennoj adaptacii k holodu [Reactions of lipid peroxidation in liver of rats at long-term adaptation to cold]. BEBiM, 1981, №4, p. 436- 437.
10. Kolosova N.G. Kolpakov A.D. Panin L.E. Soderzhanie tokoferola i perekisnoe okislenie lipidov v tkanyah krys vistar v dinamike adaptacii k holodu [The content of tocopherol and lipid peroxidation in the tissues of Wistar rats in the dynamics of adaptation to cold]. Voprosy med himii [Questions of medical chemistry]. Moscow, 1995, №6, p. 16- 19.
11. Kotelnikov V.P. Morozov V.N. Sostoyanie nejrogumoralnoj regulyacii pri otmorozhenii [The state of neurohumoral regulation at frostbite] Vestnik hirurgii im I.I Grekova [I.I. Grekov Bulletin of surgery]. Leningrad, Medicina, 1990, №2, p. 68- 71.
12. M.A. Korolyuk [i dr.] Metod opredeleniya aktivnosti katalazy [Method for determination of catalase activity]. Laboratornoe delo, 1988, №1, p. 16 – 19.
13. Morozov V.N. Hadarcev A.A. Rol sintoksicheskih i katatoksicheskih programm adaptacii v patogeneze mestnoj holodovoj travmy otmorozhenii [The role of syntoxic and catatoxic adaptation programs in the pathogenesis of local cold trauma (frostbite)]. Vestnik NMT [Herald of NMT]. Tula, 2001, V. 8, №1, p. 27 -30.
14. Mostahov I.M. Klimatogeograficheskaya harakteristika Yakutii [Climatogeografic characteristics of Yakutia]. Yakutsk, 1983, p. 245.
15. Potapova V.B. Gudkova R.B. Sokolova G.N. Osobennosti soedinitelnoj tkani slizistoj obolochki zheludka pri dlitelno ne rubcuyushchejsya yazve [Features of connective tissue of the gastric mucosa with a long-term noncicatrizing ulcer] Experimental and clin. gastroenterology. Moscow, 2004, №4, p. 24 – 26.
16. Rogozhin V.V. Metody biohimicheskih issledovanij [Methods of biochemical research]. Yakutsk, 1999, p. 93.
17. Sel’e G. Na urovne celogo organizma [At the level of the whole organism]. Moscow, Nauka, 1972, p. 121.
18. Pavlihina L.V. [i dr.] Sovremennye metody v biohimii [Modern methods in biochemistry]. Moscow, Medicina, 1977, p.147 – 151.
19. Nikolaev V.M. [i dr.] Funkcionalnaya aktivnost lejkocitov krovi i nespecificheskaya adaptivnaya reakciya krys na holod [Functional activity of blood leukocytes and nonspecific adaptive reaction of rats to cold]. Veterinariya, №1, 2016, p. 44-47.
20. Shigeev V.B. Shigeev S.V. Koludarova E.M. Holodovaya smert [Cold death]. Moscow, Novosti, 2004, 183 p.
21. Yakovleva V.I. Gvozdenko T.A. Vozrastnye aspekty sostoyaniya peroksidacii lipidov i antioksidantnoj zashchity pri dejstvii alloksana [Age aspects of the state of lipid peroxidation and antioxidant protection under the action of alloxan]. Byulleten ehksperementalnoj biologii i mediciny [Bulletin of experimental biology and medicine]. Moscow, 2005, V. 139, №3, p. 283 – 286.
22. Constantine N.G. Stanley K.R. Superoxide dismutases in hanger plants. Plant Physiol, 1977, V. 59, 565- 569.
23. Eaton J. W. Catalases and peroxidases and glutathione and hydrogen peroxide mysteries of the bestiary. J Lab Clin Med , 1991, 118, 3 – 4.
24. Scott M.D [et al.] Erythrocyte defense against hydrogen peroxide preeminent importance of catalase. J Lab Clin Med, 1991, Jul 118, 1, 7 - 16.
25. Taddei S. [et al.] Vitamin C Improves Endothelium-Dependent vasodilation by restoring nitric oxide activity in essential hypertension. Circulation, 1998, 97, 2222 – 2229.
Review
For citations:
Nikolaev V.M., Alekseev R.Z., Fedorova S.A. Intensity of lipid peroxidation in patients with cold injury. Yakut Medical Journal. 2018;(2):34-38. https://doi.org/10.25789/YMJ.2018.62.11
Views:
3
Email this article 