Changes in immune parameters and lymphocyte ATP level of peripheral blood in residents of the North during short-term cold exposure to the body
https://doi.org/10.25789/YMJ.2020.72.22
Abstract
The immune system constantly responds to various environmental stimuli. The aim of the study was to investigate immune effects of short-term cold exposure taking into account ATP level in peripheral blood lymphocytes in healthy residents of the North. So, the total number of lymphocytes and their phenotypes, as well as the content of cytokines and the concentration of ATP in the lymphocytes were determined in 38 volunteers twice (before and after their short-term stay for 5 minutes in a cold chamber at t = -25°C). Cluster analysis revealed two statistically different groups. The first group with an initially higher ATP level in lymphocytes responded to hypothermia by lower ATP concentration with the unchanging total number of lymphocytes as well as by a decrease in predominantly CD16+ killer-cells. The other group reacted by an increase in ATP concentration with a decrease in the number of lymphocytes and by a pronounced decrease in CD4+ helper-cells and in CD71+ cells with a transferrin receptor. Also, the proinflammatory cytokines TNFα and IL-6 increased in the first group, while the second group showed a decrease in the level of lymphocyte-activating cytokine IL-1β. It can be assumed that the response to hypothermia in the first group is provided through the CIRP-NFkB-TNFα axis and leads to an increase in the risk of non-infectious inflammation. For the second group, a protective mechanism is triggered to restrain lymphocyte activity and the development of T-cell-mediated inflammation through regulation by means of T-effector and T-regulatory cells AMPK balance, autophagy, mitophagy and mitochondrial biogenesis. The study of the immune response to hypothermia is important for understanding the cellular mechanisms of adaptation as well as for the search of targets to correct the immune response.
Supporting agencies: The work has been conducted as part of fundamental research program within research topic ‘Influence of body cooling on neuro-immuno-endocrine regulation of human body homeostasis’of ecological immunology laboratory of FCIARctic Institute of Physiology of Nature Adaptations. State registration № АААА-А17-117033010124-7.
About the Authors
O. V. Zubatkina
Institute of Environmental Physiology of N. Laverov Federal Center for Integrated Arctic Research Ural Department of Russian Academy of Sciences
Russian Federation
Russian Federation
Zubatkina Olga Vladimirovna - PhD, professor, research scientist of the laboratory Ecological immunology
163061, Arkhangelsk, Lomonosov ave., 249. Phone number: +79214953695
L. K. Dobrodeeva
Institute of Environmental Physiology of N. Laverov Federal Center for Integrated Arctic Research Ural Department of Russian Academy of Sciences
Russian Federation
Russian Federation
Dobrodeeva Liliya Konstantinovna - professor, chief scientist of the laboratory Ecological immunology
163061, Arkhangelsk, Lomonosov ave., 249. Phone number: 65-29-95
S. D. Kruglov
Institute of Environmental Physiology of N. Laverov Federal Center for Integrated Arctic Research Ural Department of Russian Academy of Sciences
Russian Federation
Russian Federation
Kruglov Sergey Dmitrievich - full-time postgraduate student of the laboratory Ecological immunology
163061, Arkhangelsk, Lomonosov ave., 249. Phone number: +79210782348
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Review
For citations:
Zubatkina O.V., Dobrodeeva L.K., Kruglov S.D. Changes in immune parameters and lymphocyte ATP level of peripheral blood in residents of the North during short-term cold exposure to the body. Yakut Medical Journal. 2020;(4):90-93. https://doi.org/10.25789/YMJ.2020.72.22
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