Differential microRNA expression in tumor and normal colon tissues
https://doi.org/10.25789/YMJ.2020.72.19
Abstract
Non-coding RNAs (miRNAs and long non-coding RNAs (lncRNA)) play an important role in many biological processes, and dysregulation can lead to various diseases, including colorectal cancer (CRC). The aim of the study was to analyze the differential expression of miRNAs in the tumor and normal tissues of patients with CRC, as well as the identification of potential lncRNA targets and target genes using methods of machine learning. Analysis of miRNA expression was performed by multiple parallel sequencing on a MiSeq instrument. For bioinformation analysis, DESeq2, TarPmiR, ORA (Over-Representation Analysis) and FMD (Functional module detection) algorithms were used. Sequencing revealed 6 differentially expressed microRNAs (hsa-miR-143-3p, hsa-miR-26a-5p, hsa-miR-25-3p, hsa-miR-92a-3p, hsa-miR-21-5p, hsa -let-7i-5p) in the tumor tissue of the colon is relatively normal. For these microRNAs, 97 target genes and 23 potentially interacting long non-coding RNAs were identified. Together they form a network of competitively expressed RNA characteristic of CRC, which is involved in the implementation of signaling cascades such as regulation of cell adhesion, activation of the immune response, regulation of the cellular response to hormones and stress, Wnt signaling pathway and cell migration regulation, regulation of proliferation and cell cycle, regulation interaction with viral agents, regulation of apoptosis and response to hypoxia. The data obtained expand the understanding of the mechanisms of gene expression regulation in CRC and can become the basis for a panel of tumor markers.
About the Authors
I. A. Novikova
National Medical Research Center of Oncology
Russian Federation
Russian Federation
Novikova Inna Arnoldovna ‒ Ph.D, deputy general director for science
Rostov-on-Don
N. N. Timoshkina
National Medical Research Center of Oncology
Russian Federation
Russian Federation
Timoshkina Natalia N. ‒ Ph.D., head of the laboratory of Molecular Oncology
Rostov-on-Don
D. S. Kutilin
National Medical Research Center of Oncology
Russian Federation
Russian Federation
Kutilin Denis Sergeevich ‒ Ph.D., Senior Researcher Laboratory of Molecular Oncology
Rostov-on-Don
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Review
For citations:
Novikova I.A., Timoshkina N.N., Kutilin D.S. Differential microRNA expression in tumor and normal colon tissues. Yakut Medical Journal. 2020;(4):74-82. https://doi.org/10.25789/YMJ.2020.72.19
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