Analysis of clinical outcomes in patients with gastroduodenal diseases depending on vacA Helicobacter pylori gene variants in Yakutia
N. N. Gotovtsev,
N. A. Barashkov,
V. G. Pshennikova,
T. V. Borisova,
S. N. Lekhanova,
S. A. Fedorova,
M. V. Pak,
M. P. Alexeeva,
K. S. Loskutova https://doi.org/10.25789/YMJ.2020.72.18
Abstract
A vacA gene is important virulence factor that encodes vacuolating toxin and presents in all strains of Helicobacter pylori. There is significant difference between strains in vacuolating activity determined by different sequences in the middle (m) and signal (s) regions. Helicobacter pylori vacAm has m1 and m2 alleles, vacAs has s1 and s2 alleles. The higher grade of cytotoxic activity shows Helicobacter pylori with vacAs1m1 strains than s1m2, while s2m2 has not produced vacuolating cytotoxin. The effect of vacA gene alleles on gastroduodenal diseases in patients of Yakutia earlier has not been studied. The aim of this work is an associative analysis of the clinical outcomes of the vacA Helicobacter pylori variant gene and its association with the course of gastroduodenal diseases among Yakuts. According to the results of the histological report, 154 patients (out of 322 examined) were included in the study, who had H. pylori in the antrum of the stomach. Patients were divided into two groups: chronic gastri tis (n=65) and chronic gastritis with erosive and ulcerative lesions (n=89). The average age was 26,6 years. The diagnosis of chronic gastritis was established in 65 cases (42,2%), and chronic gastritis with erosive and ulcerative lesions was diagnosed in 89 cases (57,7%). A vacAs1m1 was found in 30 out of 39 patients with erosive and ulcerative lesions (76,9%) (p< 0,05) and 9 out of 39 patients – in the sample with chronic gastritis (23,1%) (p< 0,05). Other patients (n=115) did not have statistically significant differences in comparable groups. High percentage of vacAs1m1 alleles in patients with erosive gastritis in Yakutia suggests the hypothesis that this strain is the most cytotoxic and more common for severe forms of gastritis in peptic ulcer disease.
Supporting agencies: The study was supported by the YSC KMP "Study of the genetic structure and burden of hereditary pathology of populations in the Republic of Sakha (Yakutia)" and the basic part of the state assignment of the Ministry of Science and Education of the Russian Federation (FSRG-2020-0016).
About the Authors
N. N. Gotovtsev
Federal State Budgetary Scientific Institution "Yakut Science Center of Complex Medical Problems"
Russian Federation
Russian Federation
Gotovtsev Nyurgun Naumovich – scientific researcher, Laboratory of Molecular Genetics
677010, Sakha Republic, Yakutsk, Sergelyakhskoye Shosse, 4. Phone: 89141062015
N. A. Barashkov
Federal State Budgetary Scientific Institution "Yakutsk Scientific Center of Complex Medical Problems"
Russian Federation
Russian Federation
Barashkov Nikolay Alekseevich – Candidate of biological sciences, Head of laboratory of Molecular genetics
677010, Sakha Republic, Yakutsk, Sergelyakhskoye Shosse, 4. Phone: 8-(4112) 32-19-81
V. G. Pshennikova
Federal State Budgetary Scientific Institution "Yakutsk Scientific Center for Complex Medical Problems"
Russian Federation
Russian Federation
Pshennikova Vera Gennadievna – Candidate of Biological Sciences, Head of laboratory of Populational Genetics
677010, Sakha Republic, Yakutsk, Sergelyakhskoye Shosse, 4. Phone: 8-(4112) 32-19-81
T. V. Borisova
Federal State Autonomous Educational Institution of Higher Education "North-Eastern Federal University. M.K. Ammosov "
Russian Federation
Russian Federation
Borisova Tuyara Valerievna – student, Institute of Natural Sciences
677000, Yakutsk, Kulakovsky st. 48. Phone: 89644290223
S. N. Lekhanova
Federal State Autonomous Educational Institution of Higher Education "North-Eastern Federal University M.K. Ammosov"
Russian Federation
Russian Federation
Lekhanova Sargylana Nikolaevna – Candidate of Medical Science, Associate Professor, Medical Institute
677010, Yakutsk, Oyunsky st. 27. Phone: 8-(4112) 36-30-46
S. A. Fedorova
Federal State Autonomous Educational Institution of Higher Education "North-Eastern Federal University M.K. Ammosov"
Russian Federation
Russian Federation
Fedorova Sardana Arkadievna – Doctor of biological sciences, Head of the Research Laboratory of Molecular Biology Institute of Natural Sciences
677010, Sakha Republic, Yakutsk, Kulakovsky st. 46.
M. V. Pak
Republican Hospital No. 1 National Center of Medicine
Russian Federation
Russian Federation
Pak Maria Vladimirovna – endoscopist of the endoscopic department
677010, Sakha Republic, Yakutsk, Sergelyakhskoye Shosse, 4
M. P. Alexeeva
Republican Hospital No. 1 National Center of Medicine
Russian Federation
Russian Federation
Alexeeva Mavra Pavlovna – endoscopist of the endoscopic department
677010, Sakha Republic, Yakutsk, Sergelyakhskoye Shosse, 4
K. S. Loskutova
Republican Hospital No. 1 National Center of Medicine
Russian Federation
Russian Federation
Loskutova Kiunniai Savvichna – Candidate of Medical Science, Head of the Pathoanatomical department
677010, Sakha Republic, Yakutsk, Sergelyakhskoye Shosse, 4
References
1. Baryshnikova N.V. The role of the genetic features of Helicobacter pylori in the pathogenesis of diseases in digestive system: from theory to practice / N.V. Baryshnikova, A.N. Suvorov, E.I. Tkachenko // Experimental and clinical gastroenterology (1). 2009. P. 12-19.
2. Isakov V.A. Diagnostics and treatment of infection caused by Helicobacter pylori: IV Maastricht agreement / V.A. Isakov // New recommendations for the diagnosis and treatment of H. Pylori infection - Maastricht IV (Florence). Best Clinical Practice. Russian edition. - 2012. - Issue. 2. - P. 4-23.
3. Makarenko E.V. vacA, cagA and babA genes of Helicobacter pylori in patients with duodenal ulcer and chronic gastritis / E.V. Makarenko, A.V. Voropaeva // Bulletin VGMU. 2004; VOLUME 3. No. 1.
4. Prevalence of vacA and cagA Helicobacter pylori genotypes in children and adults of the city of Vladivostok / Yu.V. Lyalikova, V.A. Miroshnichenko, N.M. Tishchenko [et al.] // Modern problems of science and education. – 2012. – № 4.
5. Comparative analysis of genotypes of Helicobacter pylori strains in Rostov and Astrakhan regions / V.M. Sorokin, R.V. Pisanov, A.S. Vodopyanov [et al.] // Medical Bulletin of the South of Russia. 2018; 9 (3): 81–86. DOI 10.21886/2219–8075–2018–9–4–81–86.
6. Characteristics of the Helicobacter pylori population in patients with diseases of the gastrointestinal tract / A.B. Zhebrun, A.V. Svarval, O.A. Balabash [et al.] // Journal of Microbiology, Epidemiology and Immunobiology. - 2013. - No. 2. - P.90-96.
7. An Overview of Helicobacter pylori VacA Toxin Biology / N.J. Foegeding, R.R. Caston, M.S. McClain [et al.] // Toxins (Basel). 2016;8(6):173. Published 2016 Jun 3. doi:10.3390/toxins8060173.
8. Clinical Relevance of the vacA, iceA, cagA, and flaA genes of Helicobacter pylori strains isolated in Eastern Taiwan / C. C. Wu, P. Y. Chou, C. T. Hu [et al.] // J. Clin. Microbiol. – 2005. – Vol. 43, № 6. – P. 2913-2915.
9. Detection of Helicobacter pylori vacA, cagA and iceA1 virulence genes associated with gastric diseases in Egyptian patients / A. El-Shenawy, M. Diab, M. Shemis [et al.] // Egyptian J Med Hum Genet. 2017;18(4):365–371. https://doi.org/10.1016/j.ejmhg.2017.04.003.
10. Effective Methods of Nucleic Acids extraction for Analysis in Molecular Biology (review) / O.S. Antonova, N.A. Korneva, Yu. V. Belov [et al.] // Scientific Instrument Making. – T. – 20. – № 1. – 2010. – P. 3-9.
11. Helicobacter pylori vacA genotypes in chronic gastritis and gastric carcinoma patients from Macau, China / I. Pinto-Ribeiro, R.M. Ferreira, S. Batalha [et al.] // Toxins. 2016;8(5):142. doi: 10.3390/toxins8050142
12. Helicobacter pylori genotypes may determinate gastric hictopathology / C. Nogueira, C. Figueiredo, F. Carneiro [et al.] // Am. J. Pathol. – 2001. – Vol. 158. – P. 647-654.
13. Helicobacter pylori vacA genotypes and cagA status and their relationship to associated diseases / P. Hou, Z.X. Tu, G.M. Xu [et al.] // World J Gastroenterol 2000; 6(4): 605-607 doi: 10.3748/wjg.v6.i4.605
14. Helicobacter pylori vacA s1m1 genotype but not cagA or babA2 increase the risk of ulcer and gastric cancer in patients from Southern Mexico / A. Román-Román, D.N. Martínez-Carrillo, J. Atrisco-Morales [et al.] // Gut Pathog. 2017 Apr 13;9:18. doi: 10.1186/s13099-017-0167-z.
15. Maeda S. Major virulence factors, VacA and CagA, are commonly positive in Helicobacter pylori isolates in Japan / S. Maeda, K. Ogura, H. Yoshida // Gut. 1998 Mar;42(3):338-43. doi: 10.1136/gut.42.3.338.
16. No correlation of babA2 with vacA and cagA genotypes of Helicobacter pylori and grading of gastritis from peptic ulcer disease patients in Brazil / R. Mattar, A.F. dos Santos, J.N. Eisig [et al.] // Helicobacter. – 2005 Dec. – 10(6). – P. 601-8. doi: 10.1111/j.1523-5378.2005.00360.x.
17. Prevalence of Helicobacter pylori vacA, cagA, cagE, iceA and babA2 genotypes in Thai dyspeptic patients / C. Chomvarin, W. Namwat, K. Chaicumpar [et al.] // Int J Infect Dis. 2008 Jan;12(1):30-6. doi: 10.1016/j.ijid.2007.03.012.
18. Ricci V. Voyage of Helicobacter pylori in human stomach: odyssey of a bacterium / V. Ricci, R. Zarrilli, M. Romano // Digest. Liver Dis. - 2002. - Vol.34, №1. - P. 2-8. DOI: 10.1016/s1590-8658(02)80051-2
19. Suerbaum S. Helicobacter pylori infection / S. Suerbaum, P. Michetti // N Engl J Med (347). – 2002. – P. 1175-1186.
20. The Helicobacter pylori vacA s1, m1 genotype and cagA is associated with gastric carcinoma in Germany / S. Miehlke, C. Kirsch, K. Agha-Amiri [et al.] // Int. J. Cancer. 2000; 87: 322-327. doi:10.1002/1097-0215(20000801)87:3<322::AIDIJC3>3.0.CO;2-M
21. Tytgat G.N. Treatment of peptic ulcer / G.N. Tytgat // Digestion. – 1998 Aug. – 59(5). – P. 446-52.
22. Yamaoka Y. Roles of the plasticity regions of Helicobacter pylori in gastroduodenal pathogenesis / Y. Yamaoka // J Med Microbiol (57). – 2008 P. – 545-553. doi: 10.1099/jmm.0.2008/000570-0.
Review
For citations:
Gotovtsev N.N., Barashkov N.A., Pshennikova V.G., Borisova T.V., Lekhanova S.N., Fedorova S.A., Pak M.V., Alexeeva M.P., Loskutova K.S. Analysis of clinical outcomes in patients with gastroduodenal diseases depending on vacA Helicobacter pylori gene variants in Yakutia. Yakut Medical Journal. 2020;(4):69-73. https://doi.org/10.25789/YMJ.2020.72.18
Views:
5
Email this article 