The ratio of peripheral blood neutrophils necrosis to apoptosis in apparently healthy northerners
https://doi.org/10.25789/YMJ.2020.72.06
Abstract
The limits and correlation of apoptosis and necrosis of neutrophil granulocytes in venous peripheral blood in apparently healthy people were studied. It was found that w contehen increasing the content of necrotic neutrophils AnV+/PI+, the concentration of apoptotic cells AnV+/PI-increases statistically to a large extent associated with the reduction of circulating neutrophils, monocytes, and t-helper cells. Thus, activation of neutrophil loss by necrosis can become a pathogenetic mechanism of cell membrane damage (nuclear, lysosomal, or cellular), as well as the formation of T-helper immunodeficiency.
Keywords
neutrophil necrosis and apoptosis,
lymphocytes,
cytokines,
immunoglobulins,
apparently healthy people
Supporting agencies: The work was carried out within the framework of the program of fundamental scientific research on the topic of the laboratory of Environmental Immunology of the Institute of Physiology of Natural Adaptations of FCIARCTIC UrO RAS № State Registration of АААА-А17-117033010124-7.
About the Authors
O. A. Stavinskaya
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Stavinskaya Olga Aleksandrovna - PhD (Biol.), senior researcher
Arkhangelsk
L. K. Dobrodeeva
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Dobrodeeva Liliya Konstantinovna - Doctor of Medical Sciences, Professor, Chief Researcher
Arkhangelsk
S. N. Balashova
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Balashova Svetlana Nikolaevna - PhD (Biol.), senior researcher
Arkhangelsk
V. P. Patrakeeva
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Patrakeeva Veronika Pavlovna - PhD (Biol.), Leading Researcher
Arkhangelsk
References
1. Gerasimov I.G. Peripheral blood neutrophil subpopulation and NST test capabilities in neonatal disease diagnosis / I.G. Gerasimov // Klin. lab. diagn. – 2011. – № 4. – P. 42-44
2. Dobrodeeva L.K. Relationship of blood cerebral natriuretic peptide levels and immune response activity in humans / L.K. Dobrodeeva, A.V. Samodova, O.E. Karyakina // Fiziologiya cheloveka. – 2016. – V. 42, № 6. – P. 106-115 doi: 10.7868/S0131164616050052
3. Lushnikov E.F. Autoliz. Morphology and mechanisms of development / E.F. Lushnikov, N.A. Shapiro. – M.: Medicina, 1974. – 199 p.
4. Pigarevskij V.E. Zenith leukocytes and their properties / V.E. Pigarevskij. – M.: Medicina, 1978. – 128 p.
5. Polikar A. Cage pathology elements / А. Polikar, М. Bessi. – M.: Medicina, 1970. – 486 p.
6. Sen'kova L.V. Physiological role of antinuclear, antiphospholipid and catalytic autoantibodies in individuals living in the North: Avtoref... dis. kand. biol. nauk. – Arhangel'sk,: 2005. – 19 p.
7. Dobrodeeva L.K. Autoantibodies content in practically healthy people / L.K. Dobrodeeva, L.V. Sen'kova, G.T. Lyutfalieva et al. // Fiziologiya cheloveka. – 2006. – V. 32, № 1. – P. 99–107
8. A method for studying the absorption and metabolic activity of peripheral blood neutrophils by phagocytosis and nst-test / Gordienko G.I. [et al.] // pat. 2249215 Rus. Federation : MPK G01N 33/52 С2
9. Todorov Y. Clinical laboratory research in pediatrics / Y. Todorov. – Sofia, 1968 – 1064 p.
10. Эйдлин Л.М. Белые тельца в крови трупа / Л.М. Эйдлин // Суд. мед. эксперт. –1928. – № 9. – С. 61-65.
11. Eidlin L.M. White bodies in the blood of a corpse / L.M. Ejdlin // Sud. med. ekspert. – 1928. – № 9. – Р. 61-65.
12. A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils / B. Geering, U. Gurzeler, E. Federzoni [et al.] // Blood. – 2011. – V. 117. – Р. 5953-5962.
13. Brostjan C. The role of neutrophil death in chronic inflammation and cancer / C. Brostjan, R. Oehler // Cell Death Discovery. – 2020. – V. 6(26). doi: 10.1038/s41420-020-0255-6
14. Changes in expression of membrane TNF, NF-κB activation and neutrophil apoptosis during active and resolved inflammation / H.L. Wright, B. Chikura, R.C. Bucknall [et al.] // Ann. Rheum. Dis. – 2011. – V. 70. – P. 537-543.
15. Cline M.J. Production and distribution of neutrophils. The white cell / M.J. Cline. –Cambridge, MA: Harvard University Press, 1975. – P. 22-38.
16. Colotta F. Modulation of granulocyte survival and programmed cell death by cytokines and bacterial products / F. Colotta, N. Polentarutti, S. Sozzani, A. Montovani // Blood. – 1992.– V. 80. – P. 2012-2020.
17. Cross A. The dual effects of TNF alpha on neutrophil apoptosis are mediated via differential effects on expression of Mcl-1 and Bfl-1 / A. Cross, R.J. Moots, S.W. Edwards // Blood. – 2008. – V. 111. – P. 878-884.
18. Differential regulation of spontaneous and immune complex-induced neutrophil apoptosis by proinflammatory cytokines. Role of oxidants, Bax and caspase-3 / L. Ottonello, G. Frumento, N. Arduino [et al.] // J. Leukoc. Biol. – 2002. – V. 72. – P. 125-132.
19. Essential Role of MMP-12 in Fas-induced Lung Fibrosis / G. Matute-Bello, M.M. Wurfel, J.S. Lee [et al.] // Cell Mol. Biol. – 2007. – V. 37. – P. 210-221. doi:10.1165/rcmb.2006-0471OC
20. Girard D. Responsiveness of human neutrophils to IL-4: induction of cytoskeletal rearrangement, de novo protein synthesis and delay of apoptosis / D. Girard, R. Paquin, A.D. Beaulieu // Biochem. J. – 1997. – V. 325. – Р. 147-153.
21. Hall C. Essential biochemistry and physiology of NT-pro-BNP / C. Hall // Eur. J. of. Heart. Fail. – 2004. – V. 3. – Р. 257-260.
22. Heinisch R.Y. Inflammatory cytokines predict one-year outcome of patient with acute coronary syndromes but not stable angina / R.Y. Heinisch, C.R. Zanetti, E. Cpmin // Eut. Ueart. J. – 2001. – V. 22. – Р. 518.
23. Human neutrophil defensins selectively chemoattract naïve T and immature dendritic cells / D. Yang, Q. Chen, O. Chertov [et al.] // J. Leukoc. Biol. – 2000. – V. 68. – P. 945-952.
24. Human neutrophil kinetics: modeling of stable isotope labeling data supports short blood neutrophil half-lives / J. Lahoz-Beneytez, M. Elemans, Y. Zhang [et al.] // Blood. – 2016. – V. 127(26). – P. 3431-3438. doi: 10.1182/blood-2016-03-700336
25. Interferon-gamma receptor 2 expression as the deciding factor in human T, B, and myeloid cell proliferation or death / P. Bernabei, E.M. Coccia, L. Rigamonti [et al.] // J. Leukoc. Biol. – 2001. – V. 70. – Р. 950-960.
26. IL-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation / S.M. Hurst, T.S. Wilkinson, R.M. McLoughlin [et al.] // Immunity. – 2001. – V. 14. – P. 705-714.
27. IL-10 Inhibits Apoptosis of Promyeloid Cells by Activating Insulin Receptor Substrate-2 and Phosphatidylinositol 3'-Kinase / J.-H. Zhou, S.R. Broussard, K. Strle [et al.] // J. Immunol. – 2001. – V. 167. – P. 4436-4442.
28. Interleukin-6 delays neutrophil apoptosis / W.L. Biffl, E.E. Moore, F.A. Moore [et al.] // Arch. Surg. – 1996. – V. 131. – Р. 24-30.
29. Interleukin-10 stimulation of phosphatidylinositol 3-kinase and p70 S6 kinase is required for the proliferative but not the antiinflammatory effects of the cytokine / J.B. Crawley, L.M. Williams, T. Mander [et al.] // J. Biol. Chem. – 1996. – V. 271. – P. 16357-16362.
30. Interleukin-10 counterregulates proinflammatory cytokine-induced inhibition of neutrophil apoptosis during severe sepsis / M. Keel, U. Ungethum, U. Steckholzer [et al.] // Blood. – 1997. – V. 90. – Р. 3356-3363.
31. Interplay between IFN-γ and IL-6 signaling governs neutrophil trafficking and apoptosis during acute inflammation / R.M. McLoughlin, J. Witowski, R.L. Robson [et al.] // J. Clin. Invest. – 2003. – V. 112. – P. 598-607.
32. Kaufmann H.S Immunologic defects in an atopic population / H.S. Kaufmann, J.R. Hobbs // Lancet. – 1970. – V. 2. – P. 1061-1076.
33. Majno G. Cellular death and necrosis; chemical, physical and morphologie change in rat liver / G. Majno, M. La Gattuta, T.E. Thompson // Arch. path. Anat. – 1960. – V. 333. – P. 421-465.
34. Mitre E. Parasite antigen-driven basophils are a major source of IL-4 in humane filarial infections / E. Mitre, R.T. Tayor // J. Immunol. – 2004. – V. 173(4). – P. 2439-2445.
35. Promotion of Neutrophil Apoptosis by TNF-α / G. Salamone, M. Giordano, A.S. Trevni [ et al.] // J. Immunol. – 2001. – V. 166(5). – P. 3476-3483.
36. Regulation of Neutrophil Apoptosis by Tumor Necrosis Factor-Alpha: Requirements for TNF-R55 and TNF-R75 for Induction of Apoptosis In Vitro / J. Murray, J. Barbara, S. Dunkley [et al.] // Blood. – 1997. – V. 90 (7). – P. 2772-2783.
37. Regulation of TNF mediated antiapoptotic signaling in human neutrophils: role of δ-PKC and ERK1/2. / L.E. Kilpatrick, S. Sun, D. Mackie et al // J. Leukoc. Biol. – 2006. – V. 80. – P. 1512-1521.
38. Sadallah S. Ectosomes relased by human neutrophils are specialized functional units / S. Sadallah, A. Hefti // J. Immunol. – 1999. – V. 163. – P. 4564-4573.
39. Toso, a functional IgM receptor, is regulated by IL-2 and T cells / Y. Murakami, S. Narayanan, S. Su [et al.] // J. Immunol. – 2012. – V. 189(2). – P. 587-597.
40. Type I and type II interferons delay human neutrophil apoptosis via activation of STAT3 and up-regulation of cellular inhibitor of apoptosis / E. Sakamoto, F. Hato, T. Kato [et al.] // J. Leukoc. Biol. – 2005. – V. 78. – P. 301-309.
41. Wei J. Autophagy regulates endoplasmic reticulum homeostasis and calcium mobilization in T-lymphocytes / J. Wei, P.H. Heather, L. Qi-Jiug // J. Immunol. – 2011. – V. 186(3). – P. 1564-1574.
Review
For citations:
Stavinskaya O.A., Dobrodeeva L.K., Balashova S.N., Patrakeeva V.P. The ratio of peripheral blood neutrophils necrosis to apoptosis in apparently healthy northerners. Yakut Medical Journal. 2020;(4):23-27. https://doi.org/10.25789/YMJ.2020.72.06
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