Evaluation of the granulocyte membrane potential of mitochondria in children with chronic inflammatory lung diseases
G. P. Evseeva,
O. A. Lebedko,
S. V. Suprun,
E. I. Yakovlev,
N. I. Kuderova,
E. V. Knizhnikova,
R. S. Telepneva,
T. V. Pivkina https://doi.org/10.25789/YMJ.2020.72.02
Abstract
The article presents the study of the characteristics of the membrane potential of mitochondria (MPM) granulocytes in 72 children with congenital lung malformations (CLM). There was a 5-fold increase in the proportion of cells with reduced granulocyte MPM(ΔΨm) compared to the control group. During the periods of exacerbation of the disease, the number of granulocytes with reduced potential increased by 8 times accompanied by the growth of luminol-and lucigenin-dependent chemiluminescence of whole blood phagocytes with spontaneous, significant decrease in the I-lum and I-luc stimulation indices by 30 and 25%. Negative dynamics of the stimulation index indicates the depletion of reserve capacities of the phagocytic system in children of the study group. There are statistically significant direct correlations between the level of granulocytes with reduced MPM(ΔΨm) and indicators of phagocytic activity of leukocytes.
About the Authors
G. P. Evseeva
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration – Research Institute of Maternity and Childhood Protection
Russian Federation
Russian Federation
Evseeva Galina Petrovna – doctor of Medical sciences, Professor, D.Sc. (Med.), Deputy Director on Scientific Work, Main Staff Scientist of the Group of Health and Environmental Problems of Mother and Child Health
O. A. Lebedko
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration – Research Institute of Maternity and Childhood Protection
Russian Federation
Russian Federation
Lebedko Olga Antonovna – doctor of Medical sciences, Professor, D.Sc. (Med.), Director
S. V. Suprun
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration – Research Institute of Maternity and Childhood Protection
Russian Federation
Russian Federation
Suprun Stefaniya Viktorovna – doctor of Medical sciences, Professor, D.Sc. (Med.), Main Staff Scientist of the Group of Health and Environmental Problems of Mother and Child Health
E. I. Yakovlev
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration – Research Institute of Maternity and Childhood Protection
Russian Federation
Russian Federation
Yakovlev Evgenii Igorevich – junior researcher of the group of medical and environmental problems of mother and child health
N. I. Kuderova
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration – Research Institute of Maternity and Childhood Protection
Russian Federation
Russian Federation
Kuderova Natalia Ivanovna – research associate of the group of clinical immunology and endocrinology
E. V. Knizhnikova
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration – Research Institute of Maternity and Childhood Protection
Russian Federation
Russian Federation
Knizhnikova Elena Vladimirovna – researcher of the Molecular Genetic Diagnostics Group
R. S. Telepneva
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration – Research Institute of Maternity and Childhood Protection
Russian Federation
Russian Federation
Telepneva Regina Sergeevna - postgraduate student of the Institute of Maternity and Childhood Protection
T. V. Pivkina
Khabarovsk Branch of Far Eastern Scientific Center of Physiology and Pathology of Respiration – Research Institute of Maternity and Childhood Protection
Russian Federation
Russian Federation
Pivkina Tatyana Vladimirovna – Researcher of Group of health and environmental problems of mother and child health
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Review
For citations:
Evseeva G.P., Lebedko O.A., Suprun S.V., Yakovlev E.I., Kuderova N.I., Knizhnikova E.V., Telepneva R.S., Pivkina T.V. Evaluation of the granulocyte membrane potential of mitochondria in children with chronic inflammatory lung diseases. Yakut Medical Journal. 2020;(4):10-13. https://doi.org/10.25789/YMJ.2020.72.02
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