Proliferation and adhesion of fibroblasts in vitro on polylactide films
https://doi.org/10.25789/YMJ.2021.74.20
Abstract
Evaluation of the proliferative activity and adhesive capacity of cultured in vitro fibroblasts on polylactide films of various thicknesses, depending on the presence of an adhesive factor was carried out.
Adding an adhesive factor AF which contains gelatin to the nutrient medium activates the proliferation of cultured cells. The largest number of attached fibroblasts is noted on the 3rd day with substrates of 100 microns and 150 microns in wells with AF, while in substrates with the thickness of 125 microns - on the 1st and 3rd days of cultivation. Based on supravitally stained samples, it was found that the fibroblast adhesion, depending on the thickness of the polylactide film, showed statistically significant differences between films with the thickness of 125 and 100 microns, as well as a tendency to increase between 125 and 150 microns. Only with the polylactide thickness of 125 microns the number of fibroblasts on the surface of the film is higher than in the well, and with other thicknesses the number of cells in the well is greater than on the polylactide.
About the Authors
A. S. Golderova
Medical Institute Federal State Autonomous Educational Institution of Higher Education M.K. Ammosov North-Eastern Federal University
Russian Federation
Russian Federation
Golderova Aitalina Semenovna - MD, professor, Head research laboratory of cell technologies and regenerative medicine
Yakutsk, Sakha Republic (Yakutia), 36 Kulakovskogo St. 677027. Tel: +7 (914) 821-59-98
I. E. Nikolaeva
Medical Institute "Federal State Autonomous Educational Institution of Higher Education M.K. Ammosov North-Eastern Federal University
Russian Federation
Russian Federation
Nikolaeva Irina Eduardovna - researcher, research laboratory of cell technologies and regenerative
Yakutsk, Sakha Republic (Yakutia), 36 Kulakovskogo St. 677027. Tel: +7 (914) 106-20-75
N. F. Timofeeva
Federal State Autonomous Educational Institution of Higher Education M.K. Ammosov North-Eastern Federal University
Russian Federation
Russian Federation
Timofeeva Nina Fedorovna - Leading Engineer of the educational scientific and technological laboratory ‘Technologies of Polymers and Nanocomposites’ and Senior Lecturer of the Chemical Department of the Institute of Natural Sciences
Yakutsk, Sakha Republic (Yakutia), 46 Kulakovskogo St. 677013. Tel: +7 (960) 978- 69-59
M. P. Kirillina
Yakutsk Scientific Center for Complex Medical Problems
Russian Federation
Russian Federation
Kirillina Maria Petrovna - candidate of biological sciences
Yakutsk, Sakha Republic (Yakutia), 6/3 Yaroslavskogo St. 677000. Tel: +7 (914) 271-68-81
A. N. Egorov
Medical Institute Federal State Autonomous Educational Institution of Higher Education M.K. Ammosov North-Eastern Federal University
Russian Federation
Russian Federation
Egorov Andrey Nikolaevich - assistant, research laboratory of cell technologies and regenerative medicine
Yakutsk, Sakha Republic (Yakutia), 36 Kulakovskogo St. 677027. Tel: +7 (914) 826-27-23
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Review
For citations:
Golderova A.S., Nikolaeva I.E., Timofeeva N.F., Kirillina M.P., Egorov A.N. Proliferation and adhesion of fibroblasts in vitro on polylactide films. Yakut Medical Journal. 2021;(2):82-85. https://doi.org/10.25789/YMJ.2021.74.20
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