Comparative analysis of biological properties of the main families of M. tuberculosis genotypes in newly diagnosed patients with respiratory tuberculosis
https://doi.org/10.25789/YMJ.2021.74.19
Abstract
The use of modern molecular genetic methods for the identification of M. tuberculosis isolates made it possible to obtain a detailed characterization of the pathogen population circulating in one of the poorly studied northern territories of Russia. The studied isolates of the M. tuberculosis population circulating in the Republic of Sakha (Yakutia) can be represented by nine main heterogeneous clusters - Beijing, T, S, Ural, Lam, Haarlem, Orphan, Uganda, X. The analysis of the massiveness and growth rate, drug resistance to anti-TB drugs of the main families of tuberculosis pathogen genotypes in newly diagnosed patients with respiratory tuberculosis showed the heterogeneity of the biological properties of M. tuberculosis. There is a connection between the viability of the pathogen and the manifestation of primary drug resistance of the M. tuberculosis population, which is clearly seen in the genotype S. Consequently, the massive growth of mycobacterium tuberculosis indicates the epidemiological danger of patients isolating these strains, especially if it is combined with rapid growth and multidrug resistance.
About the Authors
N. G. Pavlov
Research-Practice Center ‘Phthisiatry’
Russian Federation
Russian Federation
Pavlov Nikolay G. – Cand.Sci.(Veterinary Medicine), senior researcher, Bacteriologic laboratory
G. I. Alekseeva
Research-Practice Center ‘Phthisiatry’
Russian Federation
Russian Federation
Alekseeva Galina I. – Dr.Sci.(Medicine), Head of Bacteriologic laboratory
M. K. Vinokurova
Research-Practice Center ‘Phthisiatry’
Russian Federation
Russian Federation
Vinokurova Mariya K.– Dr.Sci.(Medicine), Deputy director for organization and science
M. V. Chernykh
Research-Practice Center ‘Phthisiatry’
Russian Federation
Russian Federation
Chernykh Marina V. – bacteriologist, Bacteriologic laboratory
M. V. Yakovleva
Research-Practice Center ‘Phthisiatry’
Russian Federation
Russian Federation
Yakovleva Maria V. – biologist, Bacteriologic laboratory
E. I. Ivanova
Research-Practice Center ‘Phthisiatry’
Russian Federation
Russian Federation
Ivanova Elena I. – bacteriologist, Bacteriologic laboratory
References
1. Borisov S.E. Commentary to article by Filinyuk O.V., Yanova G.V., Strelis A.K., et al. Multidrug-resistant pulmonary tuberculosis: socio-medical features and effectiveness of in-patient treatment phase // Issues of Tuberculesis – 2008. – No. 8. – P. 28.
2. Vishnevskiy A.V. Epidemiological significance of the viability and drug resistance of MTB / A.V. Vishnevskiy, T.M. Vasilyev, T.M. Golubeva, [et al.] // 5th National congress on respiratory diseases. – Moscow, 1995. – No.1747.
3. ВVirulence of M.tuberculosis / B.I. Vishnevskiy, O.V. Narvskaya, S.N. Vasilyeva, [et al.] // Issues of tuberculosis. – 2002. – No. 10. – P. 33-36.
4. Vishnevskiy B.I. Main focus areas in the work of Tuberculosis microbiology laboratory // Tuberculosis: issues in diagnostics, treatment and prevention.: proceedings of All-Russian research-practice conference. – Saint-Petersburg, 2003. – P. 34-38.
5. Vishnevskiy B.I. Mycobacterium tuberculosis virulence / B.I. Vishnevskiy, O.A. Manicheva, P.K. Yablonskiy // Infection and immunity. – 2014. – No. (4)4. – P. 319-330.
6. Grashchenkova O.V. Advancements in epidemiological analysis of tuberculosis infection / O.V. Grashchenkova, A.V. Vasilyev // Issues of tuberculosis. – 2000. – No. 6. – P. 33-36.
7. Zhdanova S.N. The deletion analysis of RD105 is a useful tool in the evaluation of Beijing strains of Mycobacterium tuberculosis / S.N. Zhdanova, O.B. Ogarkov, L.A. Stepanenko, [et al.] // Bulletin of the East Siberian Scientific Center SB RAMS. – 2011. – No. 2. – P. 194-197.
8. Koretskaya N.M. Comparative characteristics of disseminated pulmonary tuberculosis in patients isolating M.tuberculosis with high and low viability / N.M. Koretskaya, I.V. Yarygina // Issues of tuberculosis. – 2007. – No. 2. – 17-20.
9. Koretskaya N.M. Changes in the biological properties of the pathogen in infiltrative tuberculosis // N.M. Koretskaya, A.A. Chushkina // Tuberculosis and lung diseases. – 2010. – No. 9. – P. 33-38.
10. Drug sensitivity of Mycobacterium tuberculosis in relation to its viability, cytotoxicity, genotype, and clinical disease progression in patients with pulmonary tuberculosis / O.A. Manicheva, E.B. Lasunskaya, V.Y. Zhuravlev, [et al.] // Issues of Tuberculosis. – 2008. – No. 12. – P. 18-22.
11. Methods of mathematical analysis of the epidemiological situation for tuberculosis: guide for physicians. – Saint-Petersburg, 1998. – 24 p.
12. On improving anti-tuberculosis measures in Russian Federation: Order of the Russian Federation Ministry of Health no.109 (21.03.2003). Appendix 11 ‘Instructions on unified techniques for microbiological tests to detect, diagnose, and treat tuberculosis’.
13. Sapozhnikova N.V. Characteristics of pulmonary tuberculosis disease progression in relation to biological properties of the causative agent. Extended abstract of Cand.Sci.(Medicine) thesis. – Saint-Petersburg, 2003. – 24 p.
14. Tyrylgin M.A. Health protection problems in the population of the Extreme North: case of Yakutia/ Tyrylgin M.A. – Novosibirsk: Nauka Publ., 2008. – 304 p.
15. Demay, C. “SITVITWEB – a publicly available international multimarker database for studying Mycobacterium tuberculosis genetic diversity and molecular epidemiology” / C. Demay, B. Liens, T. Burguière, [et al.] // Infection, Genetics and Evolution. – 2012. – Iss. 12(4). – P. 755-766. – http://www.pasteur-guadeloupe.fr:8081/SITVIT_ONLINE/query
16. Merker, M. Evolutionary history and global spread of the Mycobacterium tuberculosis Beijing lineage / M. Merker, C. Blin, S. Mona, N. Duforet-Frebourg, S. Lecher, E. Willery, [et al.] // Nat Genet. – 2015. – Iss. 47(3). – P. 242-249. – doi: 10.1038/ng.3195.
17. Ogarkov, O. Lethal combination of mycobacterium tuberculosis Beijing genotype and human CD209-336G allele in Russian male population / O. Ogarkov, S. Zhdanova, E. Savilov, I. Mokrousov, V. Sinkov, S. Antipina // Infection, genetics and evolution. – 2012. – Iss. 12(4). – P. 732-736.
18. Weniger, T. MIRU-VNTRplus: a web tool for polyphasic genotyping of Mycobacterium tuberculosis complex bacteria / T. Weniger, J. Krawczyk, P. Supply, S. Niemann, D. Harmsen // Nucleic Acids Res. – 2010. – Iss. 38. – Suppl. W326-331. – http://www.miru-vntrplus.org/MIRU/miruinfo.faces;jsessionid=89112F274226E781C7B0B0D9118FDD70
Review
For citations:
Pavlov N.G., Alekseeva G.I., Vinokurova M.K., Chernykh M.V., Yakovleva M.V., Ivanova E.I. Comparative analysis of biological properties of the main families of M. tuberculosis genotypes in newly diagnosed patients with respiratory tuberculosis. Yakut Medical Journal. 2021;(2):76-81. https://doi.org/10.25789/YMJ.2021.74.19
Views:
11
Email this article 