Analysis of ile105val polymorphism associations of GSTP1 gene with immune reactivity markers, oxidant status and regulation of apoptosis in the children's population of the Krasnoyarsk region under aluminium bioexposure

Evaluation of the role of the Ile105Val polymorphism of the GSTP1 gene (rs1695) in the processes of cell death regulation in the child population under conditions of bioexposure to aluminum was carried out. The level of aluminum contamination in the urine of children in the observation group exceeded similar indicators in the comparison group by 1.53 times (p=0.004). A change in the ratio of immune cell populations was observed (a decrease in CD3+- and CD4+-lymphocytes, p=0.000-0.010) against the background of a violation of the overall oxidant-antioxidant balance (p=0.004-0.042). We identified the proapoptotic effects of the glutathione system associated with the increased frequency of G allele of the GSTP1 gene (rs1695), with the balance sheet of regulatory proteins Bcl-2/Bax (decrease by 2.78 times, p<0.001) and an increase in the number of apoptotic Annexinv-FITC+7ADD--cells by 2.59 times (p=0.016). Based on the results of the study, a hypothesis on the role of GSTP1 was formulated as an expression product GSTP1 gene (rs1695), in the regulation of the processes of programmed cell death with an increased level of contamination of biological media with aluminum. Thus, the G allele (105Val) of the phase II detoxification gene of glutathione-S-transferase GSTP1 can be considered as a marker of sensitivity in the examined group and can form an increased risk of immune disorders (RR=2.03; 95%CI=1.03-3.99), including dysregulation of cell death processes, under conditions of bioexposure to aluminum.

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