Comparative assessment of the intensity of oxidative stress in various experimental models

   The long-term experience of using various models of the formation of oxidative stress in a warm-blooded organism is summarized - the processes of lipid peroxidation were induced by exposure to low and high temperatures, ultraviolet irradiation, and a low-frequency alternating magnetic field. The intensity of lipid peroxidation processes in various models was assessed by the degree of accumulation of diene conjugates, lipid hydroperoxides, malondialdehyde and the level of ceruloplasmin, vitamin E, and catalase activity in the blood of laboratory animals on the 7^th, 14^th, and 21^st days of experiments. The results of a comparative assessment of the intensity of oxidative stress in various experimental models showed that the most pronounced changes in the antioxidant status are caused by the cooling of animals and exposure to ultraviolet rays, and the latter model triggers a shift in the balance to the prooxidant side by the end of the first week of the experiment, which is confirmed by the accumulation of lipid peroxidation products by 48 -61 % and a decrease in the activity of the components of the antioxidant system by 31-33 % compared with the control. Thermal exposure to rats and the effect of a low-frequency alternating magnetic field causes less pronounced, but more stable changes in the dynamics from 7 to 21 days in the state of the prooxidant/antioxidant system, which, similarly to models using hypothermia and ultraviolet light, allows us to ascertain the formation of oxidative stress.

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