Detection of beta-globin genes in the erythrocyte fraction in a patient with cervical cancer
https://doi.org/10.25789/YMJ.2022.80.27
Abstract
The results of detection of DNA fragments encoding human beta-globin and human papillomavirus L1 protein in plasma and erythrocyte samples from a cervical cancer patient using real-time polymerase chain reaction (qPCR) are presented. Amplification of a DNA fragment encoding human beta-globin was found only in a sample of erythrocytes, which may indirectly indicate the presence of extracellular DNA. The results of qPCR for the presence of a DNA fragment encoding HPV L1 were negative, which did not allow us to confirm that the isolated DNA belonged to the tumor DNA. The origin of the identified in the erythrocyte fraction DNA fragment encoding human beta-globin requires further research.
About the Authors
I. V. Kononova
Yakut Science Centre for Complex Medical Problems
Russian Federation
Russian Federation
Kononova Irina Vasilyevna – PhD in Medicine, associate researcher, acting director, Arctic Medical Center
S. N. Mamaeva
M.K. Ammosov Northeastern Federal University
Russian Federation
Russian Federation
Mamayeva Sargylana Nikolaevna – Ph.D. in Physics and Mathematics, head of the Department of the Institute of Physics and Technology
V. A. Alekseev
Yakut Science Centre for Complex Medical Problems
Russian Federation
Russian Federation
Alekseev Vladislav Amirovich – junior researcher Arctic Medical Center
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Review
For citations:
Kononova I.V., Mamaeva S.N., Alekseev V.A. Detection of beta-globin genes in the erythrocyte fraction in a patient with cervical cancer. Yakut Medical Journal. 2022;(4):104-107. https://doi.org/10.25789/YMJ.2022.80.27
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