Effect of combined sterilization on the structural and functional properties of bone implants: a study of radiation-induced changes in collagen
https://doi.org/10.25789/YMJ.2025.92.12
Abstract
This paper presents a comprehensive analysis of the effects of radiation and combined exposure on the structure and properties of bone tissue, with a particular emphasis on changes in the collagen matrix. A mathematical model has been developed describing changes in interfibrillar distances in collagen, enabling a quantitative relationship between the radiation dose and the level of protein matrix degradation. It was found that combined sterilization allows reducing the radiation dose to 12 kGy, while maintaining the structural integrity of collagen and the mechanical properties of bone material. A model of collagen degradation under the influence of radiation is proposed, linking the dose load with a change in intermolecular distances; a quantitative correlation between the parameters Δr (according to the model) and structural changes in collagen was established; the possibility of reducing the radiation dose while maintaining the sterilizing effect through ozone pretreatment has been experimentally demonstrated.
About the Authors
N. A. Nikolaeva
ФГАОУ ВО «Северо-Восточный федеральный университет имени М.К. Аммосова»
Russian Federation
Russian Federation
V. V. Rozanov
ФГБОУ ВО «Московский государственный университет имени М.В. Ломоносова»
Russian Federation
Russian Federation
S. N. Mamaeva
ФГАОУ ВО «Северо-Восточный федеральный университет имени М.К. Аммосова»
Russian Federation
Russian Federation
References
1. Brown S., Higham C., Slon V. [et al.] Identification of a new hominin bone from Denisova Cave, Siberia using collagen fingerprinting and mitochondrial DNA analysis. Scientific Reports. 2016; 6(1):23559.
2. Cho W.T., Kim S.Y., Jung S.I. [et al.] Effects of Gamma Radiation-Induced Crosslinking of Collagen Type I Coated Dental Titanium Implants on Osseointegration and Bone Regeneration. Materials (Basel). 2021; 14(12):3268.
3. Crocker D.B., Akkus O., Oest M.E. [et al.] The influence of radiation-induced collagen chain fragmentation, crosslinking, and sequential irradiation on the high-cycle fatigue life of human cortical bone. Journal of the Mechanical Behavior of Biomedical Materials. 2024; 160:106759.
4. Fan L., Ren Y., Emmert S. [et al.] The Use of Collagen-Based Materials in Bone Tissue Engineering. International Journal of Molecular Sciences. 2023; 24(4):3744.
5. Kawahara K. [et al.] Identification of animal species by the MALDI-MS of collagen in animal glues of Chinese ink sticks. Proceedings of the 59th ASMS Conference and Allied Topics, Denver, CO. 2011.
6. Nakazawa T. [et al.] Mass Spectrometry of Collagen Preserved in Neolithic Animal Bones for the Identification of Species. Proceedings of the 66th ASMS Conference and Allied Topics, San Diego, CA. 2018.
7. Ng K., Allam N., Neshatian M. [et al.] Effects of Ionizing Radiation on the Biophysical Properties of Type I Collagen Fibrils. PLOS ONE. 2025; 20(4):e0319777. https://doi.org/10.1371/journal.pone.0319777
8. Nikolaeva N.A., Rozanov V.V., Chernyaev A.P. [et al.] The influence of combined sterilization factors on the structural and functional characteristics of bone implants. International Journal of Molecular Sciences. 2023; 24(19):14426.
9. Pendleton M.M., Emerzian S.R., Liu J. [et al.] Effects of ex vivo ionizing radiation on collagen structure and whole-bone mechanical properties of mouse vertebrae. Bone. 2019; 128:115043.
10. Rozanov V.V., Matveichuk I.V., Chernyaev A.P. [et al.] Changes in the Morphological and Mechanical Characteristics of Bone Implants upon Radiation Sterilization. Bulletin of the Russian Academy of Sciences: Physics. 2019; 83(10):1311–1315.
11. Rou L., Shiqing X., Yanning G. [et al.] Application of collagen in bone regeneration. Journal of Orthopaedic Translation. 2025; 50:129–143.
Review
For citations:
Nikolaeva N.A., Rozanov V.V., Mamaeva S.N. Effect of combined sterilization on the structural and functional properties of bone implants: a study of radiation-induced changes in collagen. Yakut Medical Journal. 2025;(4):59-63. (In Russ.) https://doi.org/10.25789/YMJ.2025.92.12
Views:
22
JATS XML
Email this article 