Current capabilities of cytogenetic monitoring of ionizing radiation aneugenic influence
Abstract
Problem of assessment of occupational ionizing radiation influence on human organism requires special attention. As a result, investigations of new informative biomarkers of genotoxicological influence are particularly relevant. Numerical chromosomal abnormalities formed due to disruption of chromosome segregation machinery can be one of these markers. Modern technologies of molecular cytogenetic analysis provide a unique opportunity to register aneugenic events in human somatic cells. In tHis paper we have shown potential application of fluorescent in situ hybridization on cytokinesis-blocked lymphocytes of peripheral blood using centromere-specific DNA probes to individual chromosomes and pancentromeric DNA probes for complex assessment of ionizing radiation influence on chromosomal segregation. Special features of aneugenic effect of incorporated plutonium-239 in somatic cells of nuclear facility workers were revealed.
About the Authors
S. A. Vasilyev
НИИ медицинской генетики СО РАМН
Russian Federation
Russian Federation
V. A. Timoshevskiy
Russian Federation
I. N. Lebedev
Russian Federation
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Review
For citations:
Vasilyev S.A., Timoshevskiy V.A., Lebedev I.N. Current capabilities of cytogenetic monitoring of ionizing radiation aneugenic influence. Yakut Medical Journal. 2009;(2):145-147. (In Russ.)
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