Quantitative assessment of tumor necrosis factor induction by the whole blood cells ex vivo, in the presence of Mycobacterium tuberculosis antigens
Abstract
We studied an efficacy of whole-blood method of tumor necrosis factor-alpha (TNF-alpha) induction in the presence of Mycobacterium tuberculosis antigens (tuberculin PPD and a mixture of specific recombinant ESAT-6 and CFP-10 antigens), for differential diagnosis of active tubercular infection. A customized test system with immunoenzyme assay was developed, to quantify TNF-alpha in plasma samples. Based on findings of blood sample investigations in 46 adolescents with tubercular infection (latent infection) and active pulmonary tuberculosis, the study demonstrated the possibility of differentiation between active and latent tubercular infection, using ESAT-6 and CFP-10 antigens as TNF-alpha inducers.
About the Authors
L. I. Mordovskaya
State Institution “Research-Practice Center ‘Phthisiatry’ ”, Ministry of Health
Russian Federation
Russian Federation
Mordovskaya Larisa Ivanovna – MD, senior researcher
Sakha Republic (Yakutia)
M. A. Vladimirskii
Research Institute of Phthisiopulmonology, I.M. Sechenov First Moscow State Medical University (MSMU)
Russian Federation
Russian Federation
Vladimirskii Mikhail Alexandrovich – MD, Professor, Head of the Molecular diagnostics laboratory
Moscow
L. K. Shipina
Research Institute of Phthisiopulmonology I.M. Sechenov First Moscow State Medical University (MSMU)
Russian Federation
Russian Federation
Shipina Liubov’ Klimovna – Candidate of Biological Science, senior researcher, Molecular diagnostics laboratory
Moscow
E. I. Aksenova
Gamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of Russian Federation
Russian Federation
Russian Federation
Aksenova Ekaterina Ivanovna – researcher, Nanostructures laboratory
Moscow
A. Yu. Sazykin
Belozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
Sazykin Aleksei Yurievich – Candidate of Biological Science, associate professor, Department of Immunology
References
1. Anti-TNF immunotherapy reduces CD8+ T cell–mediated antimicrobial activity against Mycobacterium tuberculosis in humans / H. Bruns [et al.] // J. Clin. Invest. – 2009. - Vol.119(5). - P. 1167–1177.
2. Characterization of the Genes Encoding Variable Light and Heavy Chains of the HighAffinity Monoclonal Antibody Against Human Tumor Necrosis Factor / B.V. Radko [et al.] // Russian journal of Immunology – 2002. - Vol.7, № 4. - P. 371-374.
3. Dynamic changes in pro- and antiinflammatory cytokine profiles and gamma interferon receptor signaling integrity correlate with tuberculosis disease activity and response to curative treatment / E. Sahiratmadja [et al.] // Infect. Immun. – 2007. - Vol. 75, №2. – P. 820-829.
4. Increased IgG1, IFN-gamma, TNF-alpha and IL-6 responses to Mycobacterium tuberculosis antigens in patients with tuberculosis are lower after chemotherapy / A.M. Mattos [et al.] // Int. Immunol. - 2010. - Vol.22 (9). - P. 775-782.
5. Influence of disease severity on nitrite and cytokine production by peripheral blood mononuclear cells (PBMC) from patients with pulmonary tuberculosis (TB) / D.Dlugovitzky [et al.] // Clin. Exp. Immunol. – 2000. – Vol. 122, №3. – P. 343–349.
6. Molecular signatures distinguishing active from latent tuberculosis in peripheral blood mononuclear cells, after in vitro antigenic stimulation with purified protein derivative of tuberculin (PPD) or Candida: a preliminary report / J.N. Stern [et al.] // Immunol Res. – 2009. – Vol. 45, No. 1. – P. 1-12.
7. Pattern and diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease / J.S. Sutherland [et al.] // Tuberculosis. – 2009. - Vol. 89 (2). - P.136-141.
8. Treatment of rheumatoid arthritis with tumor necrosis factor inhibitors may predispose to significant increase in tuberculosis risk: a multicenter active-surveillance report / J.J.Gomez-Reino [et al.] //Arthritis Rheum. - 2003. - Vol. 48. - P. 2122–2127.
9. Tumor necrosis factor and tuberculosis / P.L. Lin [et al.] // J. Investig. Dermatol. Symp. Proc. – 2007. - Vol.12 (1). - P. 22-25.
Review
For citations:
Mordovskaya L.I., Vladimirskii M.A., Shipina L.K., Aksenova E.I., Sazykin A.Yu. Quantitative assessment of tumor necrosis factor induction by the whole blood cells ex vivo, in the presence of Mycobacterium tuberculosis antigens. Yakut Medical Journal. 2011;(4):38-40.
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