Intercellular interactions and the level of aggregation of blood cells in the area of inflammation in people living in the European North
https://doi.org/10.25789/YMJ.2023.81.22
Abstract
The immunological results of 242 people with bronchitis and 198 people with colitis, as well as 47 practically healthy people aged 21 to 55 years at the time of examination were analyzed in order to study intercellular interactions and the relative proportion of neutrophil aggregation in the area of inflammation in people living in the North. Against the background of neutropenia in the area of inflammation, the level of migration of monocytes and lymphocytes is less pronounced. In conditions close to physiological, in the exudate of the "skin window", no significant differences in the level of leukocyte migration were revealed. With neutropenia in peripheral venous blood, the content of neutrophil aggregates in the area of inflammation is higher. Aggregation of neutrophils leads to significantly more active exosecretion, degranulation and lysis of aggregate cells, which may be one of the mechanisms for the formation of neutropenia in people living in the North.
Supporting agencies: The study was performed under the program of fundamental scientific research, № 122011800217-9.
About the Authors
L. К. Dobrodeeva
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Dobrodeeva Liliya K. – MD, Professor, Chief Researcher
S. N. Balashova
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Balashova Svetlana N. – PhD in Biology, Senior Researcher
A. V. Samodova
N. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Samodova Anna V. – PhD in Biology, leading researcher
References
1. Belchenko D.I. The ratio of the intensity of exocytic lysis of erythrocytes in peripheral blood autosets and the nature of the course of acute lymphoblastic leukemia / D.I. Bel'chenko, E.L. Krivosheina // Hematology and transfusiology. 1999. No. 3. P.13-15.
2. Dobrodeeva L.K. Relationship between levels of brain natriuretic peptide in blood and immune response in subjects / L.K. Dobrodeeva, A.V. Samodova, O.E. Karyakina // Human physiology. 2016. Vol. 42, No. 6. P. 106-115. DOI: 10.7868/S0131164616050052.
3. Il’in M.V. Changes in indicators of oxidative stress and neutrophil apoptosis in diffuse and limited forms of systemic scleroderma / M.V. Iliin, P.A. Maltseva, V.A. Romanov, O.A. Khrustalev // Cytokines and inflammation. 2011. Vol. 10, No. 3. Р. 79-81.
4. Kim L.B. Oxygen transport during human adaptation to Arctic conditions and cardiorespiratory pathology / L.B. Kim. Novosibirsk: Nauka, 2015. 216 p.
5. Clinical laboratory diagnostics: research methods / I.A. Zupanets, S.V. Misyureva, V.V. Propisnova et al. Kharkiv: Golden Pages, 2005. 200 p.
6. Paltsev M.A. Intercellular interactions / M.A. Pal'tsev, A.A. Ivanov. М.: Meditsina, 1995. – 224 p.
7. Pashinskaya K.O. The effect of the content of ApoA-I in peripheral blood on the state of immune homeostasis in people living in extreme climatic conditions of the Arctic./ K.O. Pashinskaya, A.V. Samodova, L.K. Dobrodeeva // Clinical laboratory diagnostics. – 2021. – Vol. 66, No. 9. – P. 539- 545. DOI: 10.51620/0869-2084-2021-66-9-539-545.
8. Petrishcheva N.N. Hemostasis. Physiological mechanisms, principles of diagnosis of the main forms of hemorrhagic diseases / N.N. Petrishcheva, L.P. Papayan. Saint-Petersburg, 1999. 153 p.
9. Pismennaya S.V. Study of the contents of the intestine: a teaching aid / S.V. Pismennaya. Arkhangelsk, 2013. 61 p.
10. Podberezin M.M. Modern methods of diagnosis of autoimmune hemolytic anemia / M.M. Podberezin // Hematology and transfusiology. 1998. № 1. P. 15-18.
11. Roitberg G.E. Internal diseases. Laboratory and instrumental diagnostics: studies. manual / G.E. Roitberg, A.V. Strutynsky. M., 2013. 800 p.
12. Samodova A.V. Correlation of Receptors’ Extracellular Pool and Immune Response Level in Individuals Living in Arctic Conditions / A.V. Samodova, O.B. Tsypysheva // Human ecology. 2015. No. 12. P. 21-27.
13. Smirnova E.A. Exocytic erythrocytes lysis in bone marrow erythroclazic clusters – determination of its incidence for pediatric thrombocytopenic purpura prognosis and for choice of therapy. / E.A. Smirnova, D.I. Belchenko, E.L. Krivosheina // Pediatrics. 1997. Vol. 76, № 4. P. 63-65.
14. Starikova E.A. Heterogeneity of mononuclear phagocytes: subpopulations or manifestations of plasticity / E.A. Starikova, E.P. Kiseleva, I.S. Freidlin // Successes of modern biology. 2005. Vol. 125, № 5. P. 466-477.
15. Circulating immune complexes in the diagnosis of an allergic reaction of an immunocomplex type / T.N. Lebedeva, A.V. Sobolev, S.V. Minina et al. // Clinical laboratory diagnostics. 2004. № 11. P. 11-13.
16. Chernukh A.M. Inflammation / A.M. Chernukh. М.: Meditsina, 1979. 448 p.
17. Bessis M.C. Erythroblastic island, functional unity of bone marrow / M.C. Bessis // Rev Hematol. 1958. V. 13. P. 8-11.
18. Clark R.A. Chemotactic factors trigger their own oxidative inactivation by human neutrophils / R.A. Clark // J Immunol.1982. V. 129. P. 2725-2731.
19. Clark R.A. Two cytostatic components of the human neutrophil respiratory burst oxidase translocate to the plasma membrane during cell activation / R.A. Clark, B.D. Volpp, K.G. Leiddal, W.M. Nauseef // J Clin Invest. 1990. V. 85(3). P. 714-721. DOI: 10.1172/JCI114496.
20. Complement (C5a)-induced granulocyte aggregation in vitro. A possible mechanism of complement-mediated leukostasis and leukopenia / P.R. Graddock, D.E. Hammerschmidt, J.G. White [et al.] // J Clin Invest. 1977. V. 60(1). P. 260-264. DOI: 10.1172/JCI108763.
21. Consensus repeat domains of E-selectin enhance ligand binding / S.H. Li, D.K. Burns, J.M. Rumberfer [et al.] / J Biol Chem. 1994. V. 269(6). P. 4431-4437. DOI: 10.1016/s0021-9258(17)41798-4.
22. Dobrodeeva L.K. Intercellular interactions in peripheral venous blood in practically healthy residents of high latitudes / L.K. Dobrodeeva, A.V. Samodova, S.N. Balashova, K.O. Pashinskaya // BioMed Research International. 2021. V. 2021. Article ID 7086108. DOI: 10.1155/2021/7086108.
23. Expressing cloning of a receptor for C5a anaphyloxin on differentiated HL-60 cells / F. Boulay, L. Mery, M. Tardif [et al.] // Biochemistry. 1991. V. 30. P. 2993-2999. DOI: 10.1021/bi00226a002.
24. Ford W.L. The Traffic of Lymphocytes / W.L. Ford, J.L. Gowans // Semin Hematol. 1969. V. 6(1). P. 67-83.
25. Hammerschmidt D.E. Corticosteroids inhibit complement-induced granulocyte aggregation. A possible mechanism for their efficacy in chock states / D.E. Hammerschmidt, J.G. White J.G., P.R. Craddock, H.S. Jacob // J Clin Invest. 1979. V. 63(4). P. 798-803. DOI: 10.1172/JCI109365.
26. Hemodialysis leukopenia. Pulmonary vascular leukostasis resulting from complement activation by dialyzer cellophane membranes / P.R. Graddock, J. Fehr, A.P. Dalmasso [et al.] // J Clin Invest. 1977. V. 59(5). P. 879-888. DOI:10.1172/JCI108710.
27. Jandl J.H. The destruction of red cells by antibodies in man. II. Pyrogenic, leukocytic and dermal responses to immune hemolysis / J.H. Jandl, A.S. Tomlinson // J Clin Invest. 1958. V. 37(8). P. 1202-1228. DOI: 10.1172/JCI103710.
28. Korkmaz B. Neutrophil elastase, proteinase 3 and cathepsin G: physicochemical properties, activity and physiopathological functions / B. Korkmaz, T. Moreau, F. Gauthier // Biochimie. 2008. V. 90(2). P. 227-242. DOI: 10.1016/j.biochi.2007.10.009.
29. Lactoferrin is an allosteric enhancer of the proteolytic activity of cathepsin G / S. Eipper, R. Steiner, A. Lesner [et al.] // PLoS One. 2016. V. 11(3). Article ID e0151509. DOI: 10.1371/journal.pone.0151509.
30. Macrophage migration inhibitory factor sustains macrophage proinflammatory function by inhibiting p53: Regulatory role in the innate response / R.A. Mitchell, L. Hong, J. Chesney [et al.] // Proc Natl Acad Sci USA. 2002. V. 99(1). P. 345-350. DOI: 10.1073/pnas.012511599.
31. Neutrophils from burn patients are unable to increase the expression of CD11b/ CD18 in response to inflammatory stimuli / D.A. Rodeberg, R.C. Bass, J.W. Alexander [et al.] // J Leucot Biol. 1997. V. 61(5).P. 572-582. DOI: 10.1002/jlb.61.5.575.
32. Pham C.T.N. Neutrophil serine proteases: specific regulators of inflammation / C.T.N. Pham // Nat Rev Immunol. 2006. V. 6(7). P. 541-550. DOI: 10.1038/nri1841.
33. Rebuck J.W. A method of studying leukocytic functions in vivo / J.W. Rebuck, J.H. Crowley // Ann N Y Acad Sci. 1955. V. 59(5). P. 757-805. DOI: 10.1111/j.1749-6632.1955.tb45983.x.
34. Reciprocal coupling of coagulation and innate immunity via neutrophil serine proteases / S. Massberg, L. Grahl, M-L. von Bruehl [et al.] / Nat Med. 2010. V. 16(18). Р. 887-896. DOI: 10.1038/nm.2184.
35. Springer T.A. Traffic signals for lymphocytes recirculation and leukocyte emigration: the multistep paradigm / T.A. Springer // Cell. 1994. V. 76(2). P. 301-314. DOI: 10.1016/0092-8674(94)90337-9.
36. Theofilopoulus A.N. Immune complexes in human diseases: a review / A.N. Theofilopoulus, F.J. Dixon // Am J Phathol. 1980. V. 100(2). P. 529-594.
Review
For citations:
Dobrodeeva L.К., Balashova S.N., Samodova A.V. Intercellular interactions and the level of aggregation of blood cells in the area of inflammation in people living in the European North. Yakut Medical Journal. 2023;(1):87-91. https://doi.org/10.25789/YMJ.2023.81.22
Views:
43
Email this article 