DNA copy number variations (14 cancer-associated genes) in non-small cell lung cancer
https://doi.org/10.25789/YMJ.2022.79.03
Abstract
Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancers, 15-30% of which is squamous cell carcinoma, 54% is adenocarcinoma. The copy number variations (CNVs) as one of the factors affecting gene transcription activity is necessary to assess the role of genetic variation in pathological processes. The purpose of our study was to research the relative number of copies of 14 onco-associated genes: APC, AURCA, CCND1, GKN1, PIK3CA, NKX2-1, ERBB2, SOX2, EGFR1, BRCA1, BRСА2, TP63, CDKN2A, MDM2, in lung tissue samples as tumor markers of lung cancer. The study included 72 patients with Slavic and Crimean Tatar (Crimean) population, aged 46-78 (median 64) years with a diagnosis of lung cancer T1-1aN0-2M0-1 (stage I-IV). The relative copy number variation of genetic loci was assessed by the RT-qPCR method. In our study, statistically significant CNV change events (p<0.05) were recorded for the CCND1, GKN1, PIK3CA, EGFR1, SOX2, BRCA2, TP63, MDM2 genes in squamous cell carcinoma samples and NKX2-1 in lung adenocarcinoma samples. Thus, these genes can be used as differentiating and diagnostic biomarkers in NSCLC.
About the Authors
R. D. Al-Nsour
S.I. Georgievsky Medical Academy FSAI HE V.I. Vernadsky Crimea Federal Uniersity
Russian Federation
Russian Federation
Alnsour Rashed D. – postgraduate student of the Department of Oncology
N. A. Petrusenko
FSBI NMRС of oncology, Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Petrusenko Natalya Alexandrovna – junior researcher
Rostov-on-Don
V. V. Olekseenko
S.I. Georgievsky Medical Academy FSAI HE V.I. Vernadsky Crimea Federal Uniersity
Russian Federation
Russian Federation
Olekseenko Victor V. – MD, Associate Professor, Head of the Departmen
A. Y. Maksimov
FSBI NMRС of oncology, Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Maksimov Alexey Yurievich – MD, Prof. Deputy Gen. Director
Rostov-on-Don
O. N. Stateshn
FSBI NMRС of oncology, Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Stateshny Oleg Nikolayevich – oncologist
Rostov-on-Don
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Review
For citations:
Al-Nsour R.D., Petrusenko N.A., Olekseenko V.V., Maksimov A.Y., Stateshn O.N. DNA copy number variations (14 cancer-associated genes) in non-small cell lung cancer. Yakut Medical Journal. 2022;(3):11-16. https://doi.org/10.25789/YMJ.2022.79.03
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