Determination of biochemical markers of altered hepatic metabolism in white rats exposed to X-rays
R. D. Karimova,
Sh. M. Polukhova,
M. B. Zulfugarova,
F. E. Gulieva,
A. F. Rustamova,
E. M. Musayeva,
Z. G. Aliyeva,
A. V. Aslanova,
N. A. Panakhova https://doi.org/10.25789/YMJ.2025.91.06
Abstract
The article presents information about a study conducted to study changes in the level of enzymes and free products of lipid peroxidation in metabolic disorders in the liver of experimental animals exposed to X-ray radiation. The study was conducted on 42 intact white rats, which were divided into three groups. The first group (control) included 6 white rats. The second group included 18 intact white rats exposed to X-rays. In the third group, 10 days after the cessation of X-ray irradiation (18 animals), the level of liver enzymes in the blood was measured. Levels of lipid peroxidation (POL), malondialdehyde (MDA), diene conjugates (DC), hydrogen peroxide (H2O2), creatine phosphokinase (CK), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), gamma-glutamyltransferase (GGT), aspartate aminotransferase (AST) were determined in the blood of experimental animals and alanine aminotransferase (ALT).
About the Authors
R. D. Karimova
Азербайджанский медицинский университет
Azerbaijan
Azerbaijan
Sh. M. Polukhova
Азербайджанский медицинский университет
Azerbaijan
Azerbaijan
M. B. Zulfugarova
Азербайджанский медицинский университет
Russian Federation
Russian Federation
F. E. Gulieva
Азербайджанский медицинский университет
Azerbaijan
Azerbaijan
A. F. Rustamova
Азербайджанский медицинский университет
Azerbaijan
Azerbaijan
E. M. Musayeva
Азербайджанский медицинский университет
Russian Federation
Russian Federation
Z. G. Aliyeva
Азербайджанский медицинский университет
Russian Federation
Russian Federation
A. V. Aslanova
Азербайджанский медицинский университет
Russian Federation
Russian Federation
N. A. Panakhova
Азербайджанский медицинский университет
Russian Federation
Russian Federation
References
1. A new locus for otosclerosis, OTSC8, maps to the pericentromeric region of chromosome 9 / I. Bel Hadj Ali, M. Thys, N. Beltaief [et al.] // Hum Genet. 2008. 123:267–272. https://doi.org/10.1007/s00439-008-0470-3
2. A new locus for otosclerosis, OTSC10, maps to chromosome 1q41-44 / I. Schrauwen, N.J. Weegerink, E. Fransen [et al.] // Clin Genet. 2011. 79:495–497. https://doi.org/10.1111/j.1399-0004.2010.01576.x
3. A pathogenic deletion in Forkhead Box L1 (FOXL1) identifies the first otosclerosis (OTSC) gene / N. Abdelfatah, A.A. Mostafa, C.R. French [et al.] // Hum Genet. 2022. 141(3-4):965-979. doi: 10.1007/s00439-021-02381-1.
4. A second gene for otosclerosis, OTSC2, maps to chromosome 7q34-36 / K. Van Den Bogaert, P.J. Govaerts, I. Schatteman [et al.] // Am J Hum Genet. 2001. 68:495–500. https://doi.org/10.1086/318185
5. Babcock T.A., Liu X.Z. Otosclerosis: From Genetics to Molecular Biology // Otolaryngol Clin North Am. 2018. 51:305–318. https://doi.org/10.1016/j.otc.2017.11.002
6. Bravo O., Ballana E., Estivill X. Cochlear Alterations in Deaf and Unaffected Subjects Carrying the Deaf-ness-Associated A1555G Mutation in the Mitochondrial 12S rRNA Gene // Biochem Biophys Res Commun. 2006. 344, 511–516, doi:10.1016/j.bbrc.2006.03.143.
7. Candidate locus for a nuclear modifier gene for maternally inherited deafness / Y. Bykhovskaya, X. Estivill, K. Taylor [et al.] // American journal of human genetics. 2000. 66(6), 1905–1910. https://doi.org/10.1086/302914
8. Chromosomal mapping and phenotypic characterization of hereditary otosclerosis linked to the OTSC4 locus / Z. Brownstein, A. Goldfarb, H. Levi [et al.] // Arch Otolaryngol Head Neck Surg. 2006. 132:416–424. https://doi.org/10.1001/archotol.132.4.416
9. Deafness due to A1555G mitochondrial mutation without use of aminoglycoside / T. Matsunaga, H. Kumanomido, M. Shiroma [et al.] // Laryngoscope. 2004. 114. 1085-91. doi: 10.1097/00005537-200406000-00024.
10. Familial Progressive Sensorineural Deafness Is Mainly Due to the MtDNA A1555G Mutation and Is Enhanced by Treatment of Aminoglycosides / X. Estivill, N. Govea, E. Barceló [et al.] // Am J Hum Genet. 1998. 62. 27–35. doi:10.1086/301676.
11. Finsterer J. Variant m.1555A>G in MT-RNR1 causes hearing loss and multiorgan mitochondrial disorder // Medicine (Baltimore). 2020 99(6):e18488. doi: 10.1097/MD.0000000000018488.
12. Guan M.X., Fischel-Ghodsian N., Attardi G. Biochemical Evidence for Nuclear Gene Involvement in Phenotype of Non-Syndromic Deafness Associated with Mitochondrial 12S RRNA Mutation // Hum Mol Genet. 1996. 5. 963–971. doi:10.1093/hmg/5.7.963.
13. Hamasaki K., Rando R.R. Specific Binding of Aminoglycosides to a Human RRNA Construct Based on a DNA Polymorphism Which Causes Aminoglycoside-Induced Deafness // Biochemistry. 1997. 36. 12323–12328, doi:10.1021/bi970962r.
14. Heterozygous SSBP1 start loss mutation co-segregates with hearing loss and the m.1555A>G mtDNA variant in a large multigenerational family / P.J. Kullar, A. Gomez-Duran, P.A. Gammage [et al.] // Brain. 2018. 141(1):55-62. doi: 10.1093/brain/awx295.
15. High prevalence of m.1555A> G in patients with hearing loss in the Baikal Lake region of Russia as a result of founder effect / T.V. Borisova, A.M. Cherdonova, V.G. Pshennikova [et al.] // Sci Rep. 2024. 14(1):15342. doi: 10.1038/s41598-024-66254-z.
16. In Silico Model of mtDNA Mutations Effect on Secondary and 3D Structure of Mitochondrial rRNA and tRNA in Leber’s Hereditary Optic Neuropathy / B. Rovcanin, J. Jancic, J. Samardzic [et al.] // Exp Eye Res. 2020. 201. 108277, doi:10.1016/j.exer.2020.108277.
17. Linkage of otosclerosis to a third locus (OTSC3) on human chromosome 6p21.3-22.3 / W. Chen, C.A. Campbell, G.E. Green [et al.] // J Med Genet. 2002. 39:473–477. https://doi.org/10.1136/jmg.39.7.473
18. Li X., Guan M.X. A human mitochondrial GTP binding protein related to tRNA modification may modulate phenotypic expression of the deafness-associated mitochondrial 12S rRNA mutation // Mol Cell Biol. 2002. 22(21):7701-11. doi: 10.1128/MCB.22.21.7701-7711.2002.
19. Localization of a gene for otosclerosis to chromosome 15q25-q26 / M.S. Tomek, M.R. Brown, S.R. Mani [et al.] // Hum Mol Genet. 1998. 7:285–290. https://doi.org/10.1093/hmg/7.2.285
20. Mitchell P. Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism // Nature. 1961 Jul 8;191:144-8. doi: 10.1038/191144a0.
21. Mitochondrial Gene Mutation Is a Significant Predisposing Factor in Aminoglycoside Ototoxicity / N. Fischel-Ghodsian, T.R. Prezant, W.E. Chaltraw [et al.] // Am J Otolaryngol. 1997. 18. 173–178. doi:10.1016/s0196-0709(97)90078-8.
22. Mitochondrial Ribosomal RNA Gene Mutation in a Patient with Sporadic Aminoglycoside Ototoxicity // N. Fischel-Ghodsian, T.R. Prezant, X. Bu [et al.] // Am J Otolaryngol. 1993. 14. 399– 403, doi:10.1016/0196-0709(93)90113-l.
23. Mitochondrial Deafness Alleles Confer Misreading of the Genetic Code / S.N. Hobbie, C.M. Bruell, S. Akshay [et al.] // Proc Natl Acad Sci USA. 2008. 105. 3244–3249. doi:10.1073/pnas.0707265105.
24. Mitochondrial Ribosomal RNA Mutation Associated with Both Antibiotic-Induced and Non-Syndromic Deafness / T.R. Prezant, J.V. Agapian, M.C. Bohlman [et al.] // Nat Genet. 1993. 4. 289–294. doi:10.1038/ng0793-289.
25. Molecular Basis for Human Hypersensitivity to Aminoglycoside Antibiotics / T. Hutchin, I. Haworth, K. Higashi [et al.] //Nucleic Acids Res. 1993. 21, 4174–4179, doi:10.1093/nar/21.18.4174
26. Mutation of foxl1 Results in Reduced Cartilage Markers in a Zebrafish Model of Otosclerosis / A. Hawkey-Noble, J.A. Pater, R. Kollipara [et al.] // Genes (Basel). 2022. 13(7):1107. doi: 10.3390/genes13071107.
27. Mutation in TRMU related to transfer RNA modification modulates the phenotypic expression of the deafness-associated mitochondrial 12S ribosomal RNA mutations / M.X. Guan, Q. Yan, X. Li, Y. Bykhovskaya [et al.] // American journal of human genetics. 2006. 79(2), 291–302. https://doi.org/10.1086/506389
28. Otosclerosis: etiopathogenesis and histopathology / S. Cureoglu, P.A. Schachern, A. Ferlito [et al.] // Am J Otolaryngol. 2006. 27:334–340. https://doi.org/10.1016/j.amjoto.2005.11.001
29. Primer-BLAST: A Tool to Design Target-Specific Primers for Polymerase Chain Reaction / J. Ye, G. Coulouris G, I. Zaretskaya [et al.] // BMC Bioinformatics. 2012. 13, 134, doi:10.1186/1471-2105-13-134.
30. Ribosome. The Complete Structure of the 55S Mammalian Mitochondrial Ribosome / B.J. Greber, P. Bieri, M. Leibundgut [et al.] // Science. 2015. 348, 303–308, doi:10.1126/science. aaa3872.
31. Rich P.R, Maréchal A. The mitochondrial respiratory chain // Essays Biochem. 2010. 47:1-23. doi: 10.1042/bse0470001.
32. Sensorineural Hearing Loss in Patients With the m.1555A>G Mutation in the MTRNR1 Gene / J. Gallo-Terán, C. Salomón-Felechosa, R. González-Aguado [et al.] // Laryngoscope. 2024. doi: 10.1002/lary.31796.
33. Septo-optic dysplasia associated with a new mitochondrial cytochrome b mutation / M. Schuelke, H. Krude, B. Finckh [et al.] // Ann. Neurol. 2002. 51: 388-392.
34. The Genetic Landscape of Mitochondrial Diseases in Spain: A Nationwide Call / M. Bellusci, A.J. Paredes-Fuentes, E. Ruiz-Pesini [et al.] // Genes (Basel). 2021. 12. 1590. doi:10.3390/genes12101590.
35. Thys M., Van Camp G. Genetics of otosclerosis // Otol Neurotol. 2009. 30:1021–1032. https://doi.org/10.1097/MAO.0b013e3181a86509
36. Thys M., Van Den Bogaert K., Iliadou V. A seventh locus for otosclerosis, OTSC7, maps to chromosome 6q13-16.1 // Eur J Hum Genet. 2007. 15:362–368. https://doi.org/10.1038/sj.ejhg.5201761
37. Van Den Bogaert K., De Leenheer E.M., Chen W. A fifth locus for otosclerosis, OTSC5, maps to chromosome 3q22-24 // J Med Genet. 2004. 41:450–453. https://doi.org/10.1136/jmg.2004.018671
Review
For citations:
Karimova R.D., Polukhova Sh.M., Zulfugarova M.B., Gulieva F.E., Rustamova A.F., Musayeva E.M., Aliyeva Z.G., Aslanova A.V., Panakhova N.A. Determination of biochemical markers of altered hepatic metabolism in white rats exposed to X-rays. Yakut Medical Journal. 2025;(3):28-31. (In Russ.) https://doi.org/10.25789/YMJ.2025.91.06
Views:
7
Email this article 