Assessment of change of erythrocytes by method of raster electronic microscopy at the persons who died of fatal hypothermia
https://doi.org/10.25789/YMJ.2018.64.05
Abstract
Red blood cells (RBC) morphology of deceased from various causes (injury, hypothermia) was examined in this study by using scanning electron microscopy. The obtained data shows that the appearance of certain forms of cadaveric erythrocytes depends on the causes of death. Therefore, when death is caused by stabbing and gunshots RBC take acanthocyte forms and in cases of fatal hypothermia RBC take echinocyte forms. The results of an experiment carried out in vitro, at small negative temperatures,show the appearance of acanthocytes in observed blood samples. Based on theobtained data and on the ability of echinocyte to return to normal form, it can be concluded that the probability of restoring the vital activity of the frozen organismsare possible.
About the Authors
R. Z. Alekseev
YSC CMP
Russian Federation
Russian Federation
Alekseev Revo Zakharovich - MD, professor, the senior researcher
Yakutsk
A. S. Golderova
M.K. Ammosov NorthEastern Federal University
Russian Federation
Russian Federation
Golderova Aitalina Semenovna – MD, professor Institute of Physics and Technologies
Yakutsk
S. N. Mamayeva
M.K. Ammosov NorthEastern Federal University
Russian Federation
Russian Federation
Mamayeva Sargylana Nikolaevna – candidate of f-m sciences, associate professor of Institute of Physics and Technologies
Yakutsk
N. A. Nikolaeva
M.K. Ammosov North-Eastern Federal University
Russian Federation
Russian Federation
Nikolaeva Nadezhda Anatalyevna - assistant of Institute of Physics and Technologies
Yakutsk
M. T. Buzinayeva
Ministry of Health RS (Ya)
Russian Federation
Russian Federation
Buzinayeva Maria Telektesovna – candidate of medical sciences, head of the laboratory bureau of forensic medical examination
Yakutsk
References
1. Prevention of the development of necrosis during frostbite with icing of tissues / R.Z. Alekseev, M.I. Tomskij, A.S. Gol’derova [et al.] // International Journal of Applied and Fundamental Research.]. – 2015. – V. 8-1. – P. 35-41.
2. Measurement of erythrocyte morphology by electron microscopy for the diagnosis of hematuria / G.V. Maksimov, S.N. Mamaeva, S.R. Antonov [et al.] // Metrology. Quarterlyannexto J. Measuringequipment. – 2016. – V.1. – P. 47-52.
3. Foglia A. The Acanthocyte-Echinocyte differential / A. Foglia // Swiss Medical Weekly, 2010, V.140 (03-04). – P. 1-3; DOI:https://doi. org/10.4414/smw.2010.12963.
4. Bosman G.J. Erythrocyte membrane abnormalities in neuroacanthocytosis. Evidence for a neuron-erythrocyte axis? / G.J Bosman., M.W. Horstink, W.J. De Grip // Neuroacanthocytosis Syndromes. Springer Verlag. – 2005. – P.153–159.
5. Acanthocyteset hypercholesterolemia / J. Perrin, A Georges, A Morali, [et al.] // Ann Biolo Clin., 2008, V.66 (5), P. 56972.
6. Reinhart W.H. Red Cell Rheology in Stomatocyte-Echinocyte Transformation: Roles of Cell Geometry and Cell Shape / W.H. Reinhart, S.Chien // Blood., 1986. – V.4. – P. 1110–1118.
7. Wong P.A. Basis of Echinocytosis and Stomatocytosis in the Disc-sphere Transformations of the Erythrocyte. J Theor Biol., 1999, V.196(3), pp. 343–361.DOI:10,1006/jtbi.1998.0845
8. Mrowietz C, Hiebl B, Franke RP, et al. Reversibility of Echinocyte Formation after Contact of erythrocytes with various Radiographic Contrast Media. Clin. Hemorheol. Microcirc., 2008, V.39(1–4), pp. 281–286.
9. Schwarz S, Deuticke B, Haest CW. Passive Transmembrane Redistributions of Phospholipids as a Determinant of Erythrocyte Shape Change. Studieson Electroporated Cells .Mol. Membr. Biol., 1999, V.16(3), pp. 247–55.
Review
For citations:
Alekseev R.Z., Golderova A.S., Mamayeva S.N., Nikolaeva N.A., Buzinayeva M.T. Assessment of change of erythrocytes by method of raster electronic microscopy at the persons who died of fatal hypothermia. Yakut Medical Journal. 2018;(4):18-21. https://doi.org/10.25789/YMJ.2018.64.05
Views:
68
Email this article 