The characteristic and role of α-SMA-positive pancreatic stellate cells in desmoplastic reaction of stroma at pancreatic ductal adenocarcinoma and severe fibrosis at chronic pancreatitis
Abstract
The characteristics of histotopography of fibrous components and distributions of α-SMA-positive activated material are studied by methods of histochemistry molecular immunohistochemistry and digital photomorphometry with the purpose of quantitation of pancreatic stellate cells (PSC) in pancreatic ductal adenocarcinoma desmoplastic stroma and focuses with severe fibrosis (CP) in chronic pancreatitis. Indicators of relative area of Masson-positive connective fibrous components and expressions of α-isoform smooth muscle actin are calculated as well. It has been established that the α-SMA quantitative expression in activated PSC of stromal desmoplastic ductal adenocarcinoma is significantly higher than in focuses with severe fibrosis in chronic pancreatitis. Thus, the expansion in the number of α-SMA-positive activated PSC correlates directly with increasing Masson-positive connective stroma component due to the strong relation between these parameters and moderate association between these parameters in patients with chronic pancreatitis.
About the Authors
A. V. Evseev
Запорожский государственный медицинский университет
Russian Federation
Russian Federation
V. A. Tumansky
Запорожский государственный медицинский университет
Russian Federation
Russian Federation
I. S. Kovalenko
Запорожский государственный медицинский университет
Russian Federation
Russian Federation
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For citations:
Evseev A.V., Tumansky V.A., Kovalenko I.S. The characteristic and role of α-SMA-positive pancreatic stellate cells in desmoplastic reaction of stroma at pancreatic ductal adenocarcinoma and severe fibrosis at chronic pancreatitis. Yakut Medical Journal. 2015;(2):52-54. (In Russ.)
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