ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМПymj-884Research ArticleМатериалы научно-практической конференции с международным участием «Современные подход ы и перспектив ы генетических исследовани й в Якутии », 7 сентября 2017 г., г. ЯкутскMaterials of the scientific-practical conference with international participation «Modern App roaches and Prospects of Genetic Research in Yakutia», September 7, 2017, YakutskИсследование роли полиморфных вариантов генов цитокинов в развитии крапивницы в Республике БашкортостанInvestigation of the role of polymorphic variants of cytokine genes in the development of urticaria in the Republic BashkortostanГималоваГ. Ф.GimalovaG. F.

Гималова Галия Фуатовна – к.б.н., н.с. 

г. Уфа

galiyagimalova@gmail.comКарунасА. С.KarunasA. S.

Карунас Александра Станиславовна – д.б.н., зам. директора по научной работе, проф. Башкирского ГМУ

г. Уфа

carunas@list.ruХантимероваЭ. Ф.KhantimerovaE. F.

Хантимерова Эльмира Фоатовна – к.м.н., ассистент

г. Уфа

elmirka_85@mail.r0075ЗагидуллинШ. З.ZagidullinSh. Z.

Загидуллин Шамиль Зарифович – д.б.н., проф., зав. кафедрой

г. Уфа

ХуснутдиноваЭ. К.KhusnutdinovaE. K.

Хуснутдинова Эльза Камилевна – д.б.н., проф., акад. АН РБ, врио директора, зав. кафедрой БашГУ; Башкирский ГМУ

г. Уфа

Институт биохимии и генетики УНЦ РАНRussian Federation20172208202503912Copyright © Гималова Г.Ф., Карунас А.С., Хантимерова Э.Ф., Загидуллин Ш.З., Хуснутдинова Э.К., 20252025Гималова Г.Ф., Карунас А.С., Хантимерова Э.Ф., Загидуллин Ш.З., Хуснутдинова Э.К.Gimalova G.F., Karunas A.S., Khantimerova E.F., Zagidullin S.Z., Khusnutdinova E.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/884

Целью настоящей работы явилось исследование полиморфных локусов генов интерлейкинов IL4 (rs2243250), IL4R (rs1805010), IL10 (rs1800872), IL13 (rs20541) и фактора некроза опухолей TNF (rs1800629) у больных крапивницей и в контрольной группе индивидов. В результате проведенного анализа нами показано, что маркерами повышенного риска развития хронической крапивницы являются аллель rs1800629*G и генотип rs1800629*G/G полиморфного локуса гена TNF, острой крапивницы – аллель rs2243250*С гена IL4, а крапивницы с сопутствующими аллергическими заболеваниями – генотип rs1800629*G/А полиморфного локуса гена TNF. Таким образом, в данном исследовании показана ассоциация с развитием крапивницы полиморфных вариантов генов TNF и IL4.

At present, there is a steady increase in the incidence and prevalence of allergic skin diseases in the world, affecting up to 25% of the population in different countries. Urticaria is an etiologically heterogeneous group of diseases and conditions characterized by the formation of itching rashes on the skin. According to epidemiological studies, at least once during a lifetime this pathology is observed in 15-25% of the population. Urticaria is a polyethological disease. Allergic mechanisms of tissue damage are involved in the development of the allergic form of hives. Cytokines play a key role in all stages of development and maintenance of allergic inflammation. The purpose of this study was to investigate the polymorphic loci of interleukins genes IL4 (rs2243250), IL4R (rs1805010), IL10 (rs1800872), IL13 (rs20541) and tumor necrosis factor gene TNF (rs1800629) in patients with hives and in the control group of individuals. The material for the study was DNA samples of 102 unrelated individuals with urticaria, and 153 healthy individuals living in the Republic of Bashkortostan. The DNA was isolated by phenol-chloroform extraction. Genotyping of polymorphic loci was carried out by real-time PCR. As a result of the analysis, we showed that the rs1800629*G allele and the rs1800629*G/G genotype of the TNF gene polymorphism are the markers of an increased risk of developing of chronic urticaria, rs2243250*C allele of the IL4 gene – of the acute urticaria, and the rs1800629*G/A genotype of the TNF gene is a marker of the urticaria with concomitant allergic diseases development. The data obtained by us are in part consistent with the results of other authors. Thus, in patients from Japan and Canada, the SNP rs2243250 of the IL4 gene is associated with the development of atopic dermatitis. The association of the TNF gene rs1800629 polymorphism with the development of bronchial asthma and atopy is indicated in patients from the USA and Spain. As in our study, patients with allergic dermatoses from Japan did not have an association of the IL4R gene SNP rs1805010 with the development of the disease. Nevertheless, a number of other studies have shown the association with the development of various allergic diseases of all the polymorphic loci we studied. Thus, this study shows an association with the development of urticaria of polymorphic variants of the TNF and IL4 genes.

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The authors declare that there are no conflicts of interest present.