ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМП10.1234/YMJ.2022.77.02ymj-8Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHВзаимосвязь эстрадиола и вазоактивных факторов у женщин с вариантом rs 2070744 в гене эндотелиальной NO-синтазыInteraction of oestradiol and vasoactive factors in women with rs 2070744 polymorphism of endothelial NO-synthase genehttps://orcid.org/0000-0002-9346-1898БебяковаН. А.BebyakovaN. A.

Бебякова Наталья Александровна – д.б.н., проф., зав. кафедрой.

Архангельск

Nataliya A. Bebyakova – Doctor of Biological Sciences, Professor, Head of the Department of Medical Biology and Genetics, Northern Medical State University.

Arkhangelsk

nbebyakova@mail.ruhttps://orcid.org/0000-0001-5707-1836КубаА. А.KubaA. A.

Куба Анастасия Александровна – препод.-исследователь.

Архангельск

Anastasia A. Kuba – lecturer - researcher, Department of Medical Biology and Genetics, Northern Medical State University.

Arkhangelsk

ana-kuba@yandex.ruhttps://orcid.org/0000-0001-7588-4929ФадееваН. А.FadeevaN. A.

Фадеева Наталья Алексеевна – препод.-исследователь.

Архангельск

Nataliya A. Fadeeva – lecturer - researcher, Department of Medical Biology and Genetics, Northern Medical State University.

Arkhangelsk

tascha1811@rambler.ruhttps://orcid.org/0000-0001-6440-4061ХромоваА. В.KhromovaA. V.

Хромова Анна Владимировна – к.м.н., доцент, декан факультета медико-профилактического дела и медицинской биохимии.

Архангельск

Anna V. Khromova – Candidate of Medical Sciences, Associate Professor, Dean of the Faculty of Preventive Medicine and Medical Biochemistry, Northern Medical State University.

Arkhangelsk

medbio@nsmu.ruФГБОУ ВО «Северный медицинский государственный университет»РоссияNorthern Medical State UniversityRussian Federation20220202202501810Copyright © Бебякова Н.А., Куба А.А., Фадеева Н.А., Хромова А.В., 20252025Бебякова Н.А., Куба А.А., Фадеева Н.А., Хромова А.В.Bebyakova N.A., Kuba A.A., Fadeeva N.A., Khromova A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/8

Проведено исследование взаимосвязи концентрации эстрадиола с уровнем вазоактивных эндотелиальных факторов (NO, эндотелина-1) у женщин с вариантом Т−786C гена еNOS. Установлено, что взаимосвязь эстрадиола и NO, а также высокий уровень индекса периферического сопротивления, превышающий референсное значение, проявляются только при наличии в генотипе мутантного аллеля С полиморфизма Т-786С гена eNOS.

The aim of this study is the identification of interaction of oestradiol and vasoactive endothelial factors in women with polymorphism T-786C of endothelial nitric oxide synthase (eNOS) gene during follicular and luteal phase of the menstrual cycle. Sample size was 116 women with average age 19,6 years (95% CI 18,4 – 22,4), being born and living in the circumpolar region. Genotyping of T-786C polymorphism of eNOS gene was performed by pyrosequencing. The peripheral vascular tone was evaluated by tetrapolar reography. Concentration of nitric oxide (NO) was measured by Griss reaction. The level of oestradiol and endothelin-1 (ET-1) was measured by ELISA (enzyme-linked immunosorbent assay). Index ratio vasodilator to vasoconstrictor was calculated (NO/ET-1). «SPSS statistics» (StatSoft, USA) was used for statistical analyses. Our study found that the frequency of T allele was 0,67, C allele – 0,33 and C/C genotype was the rarest. The highest concentration of NO was found in women with C/C genotype during follicular phase. The concentration of NO in luteal phase in women with different genotypes of eNOS gene was similar. The level of ET-1 in women with T/T and C/T genotypes was normal during follicular and luteal phase, but in women with C/C genotype the level of ET-1 was above the normal reference range during follicular and luteal phase. The study of peripheral vascular tone found that C/C genotype was correlated with higher level of index of peripheral resistance (IPR) during follicular phase after dosed physical test, higher level of IPR was before and after dosed physical testduring luteal phase. The concentration of oestradiol in women with T/T and C/T genotypes was higher during luteal phase, in women with C/C genotype the level of oestradiol was higher in follicular phase. Correlation analysis between oestradiol and NO determined the moderate correlation (r=0,302; p=0,05) in C/T genotype, the strong positive association in C/C genotype during follicular phase (r=0,755, p=0,03) and luteal phase, and no association in T/T genotype. Thus, it was revealed that the presence of both mutant alleles C was correlated with imbalance of vasoactive factors (higher level of vasoconstrictors), leading to higher vascular peripheral tone. Also it was found that the association between oestradiol and NO manifested only in case of presence of mutant allele C of polymorphism T-786C gene of eNOS in genotype, that can approve protective function of oestradiol in relation to NO synthesis and can be confirmed by higher level of NO in women with C/C genotype during follicular phase of cycle.

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The authors declare that there are no conflicts of interest present.