ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМП10.25789/YMJ.2020.72.22ymj-733Research ArticleАРКТИЧЕСКАЯ МЕДИЦИНАARCTIC MEDICINEИзменение иммунных показателей и уровня АТФ лимфоцитов периферической крови у северян при кратковременном холодовом воздействии на организмChanges in immune parameters and lymphocyte ATP level of peripheral blood in residents of the North during short-term cold exposure to the bodyhttps://orcid.org/0000-0002-5039-2220ЗубаткинаО. В.ZubatkinaO. V.

Зубаткина Ольга Владимировна – д.б.н., проф., с.н.с.

Zubatkina Olga Vladimirovna - PhD, professor, research scientist of the laboratory Ecological immunology

163061, Arkhangelsk, Lomonosov ave., 249. Phone number: +79214953695

ozbiochem@gmail.comhttps://orcid.org/0000-0001-5080-6502ДобродееваЛ. К.DobrodeevaL. K.

Добродеева Лилия Константиновна – д.м.н., проф., гл.н.с., директор Института

Dobrodeeva Liliya Konstantinovna - professor, chief scientist of the laboratory Ecological immunology

163061, Arkhangelsk, Lomonosov ave., 249. Phone number: 65-29-95

https://orcid.org/0000-0002-4085-409XКругловС. Д.KruglovS. D.

Круглов Сергей Дмитриевич – аспирант

Kruglov Sergey Dmitrievich - full-time postgraduate student of the laboratory Ecological immunology

163061, Arkhangelsk, Lomonosov ave., 249. Phone number: +79210782348

stees67@yandex.ruИнститут физиологии природных адаптаций ФГБУН ФИЦ комплексного изучения Арктики им. акад. Н.П. Лаверова УрО РАНРоссияInstitute of Environmental Physiology of N. Laverov Federal Center for Integrated Arctic Research Ural Department of Russian Academy of SciencesRussian Federation202002062025049093Copyright © Зубаткина О.В., Добродеева Л.К., Круглов С.Д., 20252025Зубаткина О.В., Добродеева Л.К., Круглов С.Д.Zubatkina O.V., Dobrodeeva L.K., Kruglov S.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/733

С целью установления характера иммунного реагирования на кратковременное холодовое воздействие с учетом уровня АТФ в лимфоцитах периферической крови у практически здоровых северян были обследованы жители г. Архангельска обоего пола, средний возраст которых составил 35 (8,7) лет.Для одной группы обследуемых с изначально более высоким уровнем АТФ в лимфоцитах ответ на гипотермию охарактеризовался снижением концентрации АТФ при неменяющемся общем количестве лимфоцитов и снижением преимущественно фенотипа CD16+ клетоккиллеров. Реакция другой группы проявилась ростом концентрации АТФ при уменьшении числа лимфоцитов и выраженным снижением CD4+ клеток-хелперов и CD71+ клеток с рецептором к трансферрину. Также в первой группе повысилось содержание провоспалительных цитокинов TNFα и IL-6, в то время как во второй группе наблюдалось снижение уровня лимфоцитактивирующего цитокина IL-1β.

The immune system constantly responds to various environmental stimuli. The aim of the study was to investigate immune effects of short-term cold exposure taking into account ATP level in peripheral blood lymphocytes in healthy residents of the North. So, the total number of lymphocytes and their phenotypes, as well as the content of cytokines and the concentration of ATP in the lymphocytes were determined in 38 volunteers twice (before and after their short-term stay for 5 minutes in a cold chamber at t = -25°C). Cluster analysis revealed two statistically different groups. The first group with an initially higher ATP level in lymphocytes responded to hypothermia by lower ATP concentration with the unchanging total number of lymphocytes as well as by a decrease in predominantly CD16+ killer-cells. The other group reacted by an increase in ATP concentration with a decrease in the number of lymphocytes and by a pronounced decrease in CD4+ helper-cells and in CD71+ cells with a transferrin receptor. Also, the proinflammatory cytokines TNFα and IL-6 increased in the first group, while the second group showed a decrease in the level of lymphocyte-activating cytokine IL-1β. It can be assumed that the response to hypothermia in the first group is provided through the CIRP-NFkB-TNFα axis and leads to an increase in the risk of non-infectious inflammation. For the second group, a protective mechanism is triggered to restrain lymphocyte activity and the development of T-cell-mediated inflammation through regulation by means of T-effector and T-regulatory cells AMPK balance, autophagy, mitophagy and mitochondrial biogenesis. The study of the immune response to hypothermia is important for understanding the cellular mechanisms of adaptation as well as for the search of targets to correct the immune response.

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The authors declare that there are no conflicts of interest present.