ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМП10.25789/YMJ.2024.87.03ymj-5Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHСоотношение показателей капиллярной и венозной периферической крови, оценка их изменения после общего кратковременного охлажденияThe ratio of capillary and venous peripheral blood, assessment of their changes after short-term coolingПатракееваВ. П.PatrakeevaV. P.

Патракеева Вероника Павловна – к.б.н., в.н.с., зав. лаб.

Patrakeeva Veronika Pavlovna – Сandidate of Biological Sciences, leading researcher, Head of the Laboratory of Ecological Immunology.

patrakeewa.veronika@yandex.ruКонтиевскаяЕ. В.KontievskayaE. V.

Контиевская Елена Владимировна – м.н.с.

Kontievskaya Elena Vladimirovna – junior researcher.

kaiyo-kato@yandex.ruКарякинаО. Е.KaryakinaO. E.

Карякина Ольга Евгеньевна – к.б.н., с.н.с.

Karyakina Olga Evgenievna – Candidate of Biological Science, senior researcher.

novogil@mail.ruФИЦ им. акад. Н.П. Лаверова УрО РАН, Ин-т физиологии природных адаптацийРоссияN. Laverov Federal Center for Integrated Arctic Research of the Ural Branch of the Russian academy of sciencesRussian Federation202402022025031824Copyright © Патракеева В.П., Контиевская Е.В., Карякина О.Е., 20252025Патракеева В.П., Контиевская Е.В., Карякина О.Е.Patrakeeva V.P., Kontievskaya E.V., Karyakina O.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/5

Проведен сравнительный анализ показателей капиллярной и венозной крови у практически здоровых людей до и после общего охлаждения. Установлено, что в капиллярной крови более высокий уровень лейкоцитов обеспечивается преимущественно за счет зрелых форм моноцитов, нейтрофилов, эозинофилов и базофилов. Значимых различий в уровне лимфоцитов не установлено. Реакцией на охлаждение, в зависимости от гематологической пробы, является повышение циркулирующего пула лейкоцитов в капиллярной крови за счет усиления миграции клеток и активации рециркуляции лимфоцитов, а венозной – повышение выхода из депо нейтрофилов. Эритроциты капиллярной крови имеют большую вариативность по размеру. После охлаждения эритроцитарные показатели капиллярной и венозной крови имеют одинаковую тенденцию к повышению, с более высоким темпом прироста в капиллярной пробе, что может отражать значимость их в регуляции гомеостаза мелких сосудов при охлаждении. В капиллярной крови уровень тромбоцитов ниже, но популяция их более гетерогенна и выше содержание крупных клеток. При общем охлаждении тромбоцитарные показатели вне зависимости от гематологической пробы значимо не изменились. Таким образом, изменения в составе венозной крови отражают классическую реакцию на стресс с повышением уровня сегментоядерных нейтрофилов. Изменения показателей капиллярной крови направлены на поддержание гомеостаза мелких сосудов, повышение пула функционально активных и рециркулирующих клеток, обеспечивающих эффективный ответ на антигенное воздействие.

A comparative analysis of capillary and venous blood parameters in practically healthy people before and after general cooling was carried out. It has been established that in capillary blood a higher level of leukocytes is provided mainly by mature forms of monocytes, neutrophils, eosinophils and basophils. There were no significant differences in the level of lymphocytes. The response to cooling, depending on the hematological test, is an increase in the circulating pool of leukocytes in capillary blood due to increased cell migration and activation of lymphocyte recycling, and in venous blood an increase in the output of neutrophils from the depot. Red blood cells in capillary blood have a large degree of variation in size. After cooling, erythrocyte indices of capillary and venous blood have the same tendency to increase, with a higher rate of increase in the capillary sample, which may reflect their importance in the regulation of the homeostasis of small vessels during cooling. In capillary blood the level of platelets is lower, but their population is more heterogeneous and the content of large cells is higher. With general cooling, platelet parameters, regardless of the hematological test, did not change significantly. Thus, changes in the composition of venous blood reflect the classic response to stress with an increase in the level of segmented neutrophils. Changes in capillary blood parameters are aimed at maintaining the homeostasis of small vessels, increasing the pool of functionally active and recirculating cells that provide an effective response to antigenic influence.

капиллярная кровьвенозная кровьлейкограммагемограммаобщее охлаждениеадаптацияcapillary bloodvenous bloodleukogramhemogramgeneral coolingadaptationРабота выполнена в рамках программы фундаментальных научных исследований по теме лаборатории экологической иммунологии Института физиологии природных адаптаций ФГБУН ФИЦКИА УрО РАН № гос. регистрации 122011300377-5ReferencesГематологические показатели клинически здоровых лиц / Мингачева А.А., Гергель Н.И., Косов А.И., Кудинова Е.В., Карслян Л.С., Косякова Ю.А.// Медицинский альманах. 2011. № 3(16). С. 175-176.Hematological indicators of health of healthy people / Mingacheva A.A., Gergel N.I., Kosov A.I., Kudinova E.V., Karslyan L.S., Kosyakova Yu.A. // Medical almanac. 2011. No. 3(16). P. 175-176.К вопросу об оптимизации работы КДЛ: сравнительная оценка показателей венозной и капиллярной крови / Мингачева А.А., Максимов А.Б., Зиманова О.Г., Бабичев А.В., Баишева Г.М., Первова Ю.В. // Ремедиум Приволжье. 2014. С. 33-35.On the issue of optimizing the work of CDL: comparative assessment of venous and capillary blood parameters / Mingacheva A.A., Maksimov A.B., Zimanova O.G., Babichev A.V., Baisheva G.M., Pervova Yu. IN. // Remedium Volga region. 2014. P.33-35.Мингачева А.А. Сравнительная оценка форменных элементов венозной и капиллярной крови // Аспирантский вестник Поволжья 2011. № 1-2. С.193-194.Mingacheva A.A.comparative assessment of the formed elements of venous and capillary blood // Postgraduate Bulletin of the Volga region. 2011. No.1-2. P. 193-194.Патракеева В.П., Самодова А.В. Влияние кратковременного общего охлаждения на миграцию, рециркуляцию и энергетический ресурс иммунокомпетентных клеток периферической крови человека // Вестн. Уральской медицин. академ. науки. 2017. Т. 14. № 4. С. 362-368. DOI: 10.22138/2500-0918-2017-14-4-362-368Patrakeeva V.P., Samodova A.V. The influence of short-term general cooling on the migration, recycling and energy resource of immunocompetent cells of human peripheral blood // Bulletin of the Ural Medical Academic Science. 2017. T. 14. No. 4. P. 362-368. DOI: 10.22138/2500-0918-2017-14-4-362-368A comparison between haematological parameters in 'capillary' and venous blood from healthy adults / Daae L.N., Halvorsen S., Mathisen P.M., Mironska K.//scand J Clin Lab Invest. 1988. № 48(7). P. 723-6. doi:10.1080/00365518809085796.A comparison between haematological parameters in 'capillary' and venous blood from healthy adults / Daae L.N., Halvorsen S., Mathisen P.M., Mironska K.//scand J Clin Lab Invest. 1988. № 48(7). P. 723-6. doi:10.1080/00365518809085796.A practical approach for complete blood count analysis following acute exercise: Capillary vs. venous blood sampling / L.C. Bates-Fraser, K.M. Moertl, C.K. Stopforth, D.B. Bartlett, K.S. Ondrak, B.C. Jensen, E.D. Hanson. // Advanced Exercise and Health Science. 2024. V. 1. No.1. P. 43-50. https://doi.org/10.1016/j.aehs.2024.01.002A practical approach for complete blood count analysis following acute exercise: Capillary vs. venous blood sampling / L.C. Bates-Fraser, K.M. Moertl, C.K. Stopforth, D.B. Bartlett, K.S. Ondrak, B.C. Jensen, E.D. Hanson. // Advanced Exercise and Health Science. 2024. V. 1. No.1. P. 43-50. https://doi.org/10.1016/j.aehs.2024.01.002Acute stress increases monocyte levels and modulates receptor expression in healthy females. / M. van de Wouw [et al.] // Brain, Behavior, and Immunity. 2021. V. 94. P. 463-468. https:// doi.org/10.1016/j.bbi.2021.03.005Acute stress increases monocyte levels and modulates receptor expression in healthy females. / M. van de Wouw [et al.] // Brain, Behavior, and Immunity. 2021. V. 94. P. 463-468. https:// doi.org/10.1016/j.bbi.2021.03.005Analysis of common biomarkers in capillary blood in routine clinical laboratory. Preanalytical and analytical comparison with venous blood / Maroto-García J., Deza S., Fuentes-Bullejos P., Fernández-Tomás P., Martínez-Espartosa D., Marcos-Jubilar M., Varo N., González Á. // Diagnosis (Berl). 2023. No.10(3). P. 281-297. doi: 10.1515/dx-2022-0126.Analysis of common biomarkers in capillary blood in routine clinical laboratory. Preanalytical and analytical comparison with venous blood / Maroto-García J., Deza S., Fuentes-Bullejos P., Fernández-Tomás P., Martínez-Espartosa D., Marcos-Jubilar M., Varo N., González Á. // Diagnosis (Berl). 2023. No.10(3). P. 281-297. doi: 10.1515/dx-2022-0126.Chronic stress increases metastasis via neutrophil-mediated changes to the microenvironment / He X.Y. [et al.] // Cancer Cell. 2024. V. 42(3). P. 474-486.e12.https://doi.org/10.1016/j.ccell.2024.01.013Chronic stress increases metastasis via neutrophil-mediated changes to the microenvironment / He X.Y. [et al.] // Cancer Cell. 2024. V. 42(3). P. 474-486.e12.https://doi.org/10.1016/j.ccell.2024.01.013comparison of blood counts in venous, fingertip and arterial blood and their measurement variation / Yang Z. W., Yang S. H., Chen L., Qu J., Zhu J., Tang Z. // Clin Lab Haematol. 2001. No.23(3). P. 155-9. doi: 10.1046/j.1365-2257.2001.00388.x.comparison of blood counts in venous, fingertip and arterial blood and their measurement variation / Yang Z. W., Yang S. H., Chen L., Qu J., Zhu J., Tang Z. // Clin Lab Haematol. 2001. No.23(3). P. 155-9. doi: 10.1046/j.1365-2257.2001.00388.x.comparison of complete blood count parameters between venous and capillary blood in oncology patients / Chavan P., Bhat V., Tiwari M., Gavhane U., Pal S.K. // J Lab Physicians. 2016. No. 8. P. 65-66. doi:10.4103/0974-2727.176238.comparison of complete blood count parameters between venous and capillary blood in oncology patients / Chavan P., Bhat V., Tiwari M., Gavhane U., Pal S.K. // J Lab Physicians. 2016. No. 8. P. 65-66. doi:10.4103/0974-2727.176238.Differences in lipid and lipoprotein concentrations of capillary and venous blood samples / Kupke I. R., Zeugner S., Gottschalk A., Kather B. // Clin Chim Acta. 1979. No. 97(2-3). P. 279-83. doi: 10.1016/0009-8981(79)90426-1.Differences in lipid and lipoprotein concentrations of capillary and venous blood samples / Kupke I. R., Zeugner S., Gottschalk A., Kather B. // Clin Chim Acta. 1979. No. 97(2-3). P. 279-83. doi: 10.1016/0009-8981(79)90426-1.Falch D. K. Clinical chemical analyses of serum obtained from capillary versus venous blood, using Microtainers and Vacutainers //scand J Clin Lab Invest. 1981. No. 41(1). P. 59-62. doi: 10.3109/00365518109092015.Falch D. K. Clinical chemical analyses of serum obtained from capillary versus venous blood, using Microtainers and Vacutainers //scand J Clin Lab Invest. 1981. No. 41(1). P. 59-62. doi: 10.3109/00365518109092015.Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies /s. Baggio, G. Togni, I. Eckerle, N. Vuillemier, L. Kaiser, L. Gétaz // Practical Laboratory Medicine. 2022. No.31(1). e00290. DOI:10.1016/j.plabm.2022.e00290.Feasibility of home-based ELISA capillary blood self-testing for anti-SARS-CoV-2 antibodies /s. Baggio, G. Togni, I. Eckerle, N. Vuillemier, L. Kaiser, L. Gétaz // Practical Laboratory Medicine. 2022. No.31(1). e00290. DOI:10.1016/j.plabm.2022.e00290.Feasibility of microvolumetric capillary whole blood collections for usage in Athlete Biological Passport analysis / J.M. Goodrum, L.A. Lewis, M.N. Fedoruk, D. Eichner, G.D. Miller // Drug Test Anal. 2022. No. 14(7). P. 1291-1299. doi: 10.1002/dta.3254.Feasibility of microvolumetric capillary whole blood collections for usage in Athlete Biological Passport analysis / J.M. Goodrum, L.A. Lewis, M.N. Fedoruk, D. Eichner, G.D. Miller // Drug Test Anal. 2022. No. 14(7). P. 1291-1299. doi: 10.1002/dta.3254.Gowans J. L. The lymphocyte — a disgraceful gap in medical knowledge // Immunology Today. 1996. V. 17(6). P. 288-291. doi.org/10.1016/0167-5699(96)80547-0.Gowans J. L. The lymphocyte — a disgraceful gap in medical knowledge // Immunology Today. 1996. V. 17(6). P. 288-291. doi.org/10.1016/0167-5699(96)80547-0.Howell A., Arsic N., Griebel P. Resting and activated bovine neutrophils and eosinophils differ in their responses to adrenergic agonists / Veterinary Immunology and Immunopathology // 2024. V. 272. e110758. https://doi.org/10.1016/j.vetimm.2024.110758.Howell A., Arsic N., Griebel P. Resting and activated bovine neutrophils and eosinophils differ in their responses to adrenergic agonists / Veterinary Immunology and Immunopathology // 2024. V. 272. e110758. https://doi.org/10.1016/j.vetimm.2024.110758.Kupke I.R., Kather B., Zeugner S. On the composition of capillary and venous blood serum // Clin Chim Acta. 1981. No. 112(2). P.177-85. doi:10.1016/0009-8981(81)90376-4.Kupke I.R., Kather B., Zeugner S. On the composition of capillary and venous blood serum // Clin Chim Acta. 1981. No. 112(2). P.177-85. doi:10.1016/0009-8981(81)90376-4.Li R., Zeng J., Ren T. International Immunopharmacology. 2022. V. 110. e108961. Expression of DEL-1 in alveolar epithelial cells prevents lipopolysaccharide-induced inflammation, oxidative stress, and eosinophil recruitment in acute lung injury. https://doi.org/10.1016/j.intimp.2022.108961Li R., Zeng J., Ren T. International Immunopharmacology. 2022. V. 110. e108961. Expression of DEL-1 in alveolar epithelial cells prevents lipopolysaccharide-induced inflammation, oxidative stress, and eosinophil recruitment in acute lung injury. https://doi.org/10.1016/j.intimp.2022.108961Low shear stress exacerbates atherosclerosis by inducing the generation of neutrophil extracellular traps via Piezo1-mediated mechanosensation / Y. Zhu [et al.] // Atherosclerosis. 2024. V. 391. e117473. https://doi.org/10.1016/j.atherosclerosis.2024.117473Low shear stress exacerbates atherosclerosis by inducing the generation of neutrophil extracellular traps via Piezo1-mediated mechanosensation / Y. Zhu [et al.] // Atherosclerosis. 2024. V. 391. e117473. https://doi.org/10.1016/j.atherosclerosis.2024.117473Macleod K., Katz L.B., Cameron H. Capillary and Venous Blood Glucose Accuracy in Blood Glucose Meters Versus Reference Standards: The Impact of Study Design on Accuracy Evaluations // J Diabetes Sci Technol. 2019. 13(3). P. 546-552. doi: 10.1177/1932296818790228.Macleod K., Katz L.B., Cameron H. Capillary and Venous Blood Glucose Accuracy in Blood Glucose Meters Versus Reference Standards: The Impact of Study Design on Accuracy Evaluations // J Diabetes Sci Technol. 2019. 13(3). P. 546-552. doi: 10.1177/1932296818790228.Red blood cells from endothelial nitric oxide synthase-deficient mice induce vascular dysfunction involving oxidative stress and endothelial arginase I / Z. Zhuge [et al.] // Redox Biology. 2023. V. 60. e102612. doi.org/10.1016/j.redox.2023.102612Red blood cells from endothelial nitric oxide synthase-deficient mice induce vascular dysfunction involving oxidative stress and endothelial arginase I / Z. Zhuge [et al.] // Redox Biology. 2023. V. 60. e102612. doi.org/10.1016/j.redox.2023.102612Shapiro K. E., Buhimschi I.A., Fleisher J. Accuracy of anemia screening by point-of-care hemoglobin testing in patients seeking abortion // Contraception. 2022. V. 105. P. 51-54. https://doi.org/10.1016/j.contraception.2021.09.003Shapiro K. E., Buhimschi I.A., Fleisher J. Accuracy of anemia screening by point-of-care hemoglobin testing in patients seeking abortion // Contraception. 2022. V. 105. P. 51-54. https://doi.org/10.1016/j.contraception.2021.09.003Significant differences between capillary and venous complete blood counts in the neonatal period / Kayiran S.M., Ozbek N., Turan M., Gürakan B. // Clin Lab Haematol. 2003. No. 25(1). P. 9-16. doi: 10.1046/j.1365-2257.2003.00484.x.Significant differences between capillary and venous complete blood counts in the neonatal period / Kayiran S.M., Ozbek N., Turan M., Gürakan B. // Clin Lab Haematol. 2003. No. 25(1). P. 9-16. doi: 10.1046/j.1365-2257.2003.00484.x.Single-cell transcriptome profiling reveals vascular endothelial cell heterogeneity in human skin. / Q. Li, Z. Zhu, L. Wang, Y. Lin, H. Fang, J. Lei, T. Cao, G. Wang, E. Dang // Theranostics. 2021. No. 11(13). P. 6461-6476. doi: 10.7150/thno.54917.eCollection 2021Single-cell transcriptome profiling reveals vascular endothelial cell heterogeneity in human skin. / Q. Li, Z. Zhu, L. Wang, Y. Lin, H. Fang, J. Lei, T. Cao, G. Wang, E. Dang // Theranostics. 2021. No. 11(13). P. 6461-6476. doi: 10.7150/thno.54917. eCollection 2021Stability and comparison of complete blood count parameters between capillary and venous blood samples / M.J.H. Doeleman, A. Esseveld, A. Huisman, S. de Roock, W.M.T. Groenestege // Int J Lab Hematol. 2023. No. 45(5). P. 659-667. doi: 10.1111/ijlh.14080.Stability and comparison of complete blood count parameters between capillary and venous blood samples / M.J.H. Doeleman, A. Esseveld, A. Huisman, S. de Roock, W.M.T. Groenestege // Int J Lab Hematol. 2023. No. 45(5). P. 659-667. doi: 10.1111/ijlh.14080.The relationship between interleukin-6 in saliva, venous and capillary plasma, at rest and in response to exercise / T. Cullen, A.W. Thomas, R. Webb, M.G. Hughes // Cytokine. 71(2). 2015. Р. 397-400. doi: 10.1016/j.cyto.2014.10.011.The relationship between interleukin-6 in saliva, venous and capillary plasma, at rest and in response to exercise / T. Cullen, A.W. Thomas, R. Webb, M.G. Hughes // Cytokine. 71(2). 2015. Р. 397-400. doi: 10.1016/j.cyto.2014.10.011.Venous versus capillary sampling for total creatine kinase assay: Effects of a simulated football match / D.C.X. de Oliveira, A. Frisselli, E.G. de Souza, L.C.R. Stanganelli, R. Deminice // PLoS One. 2018. No. 13(9). e0204238. doi: 10.1371/journal.pone.0204238.Venous versus capillary sampling for total creatine kinase assay: Effects of a simulated football match / D.C.X. de Oliveira, A. Frisselli, E.G. de Souza, L.C.R. Stanganelli, R. Deminice // PLoS One. 2018. No. 13(9). e0204238. doi: 10.1371/journal.pone.0204238.Woolley T., Rutter E., Staudenmaier M. Comparability and stability of serum creatinine concentration in capillary and venous blood // Br J Biomed Sci. 2023. No. 80. P. 11402. doi: 10.3389/bjbs.2023.11402.Woolley T., Rutter E., Staudenmaier M. Comparability and stability of serum creatinine concentration in capillary and venous blood // Br J Biomed Sci. 2023. No. 80. P. 11402. doi: 10.3389/bjbs.2023.11402.

The authors declare that there are no conflicts of interest present.