ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМП10.25789/YMJ.2022.80.27ymj-325Research ArticleТОЧКА ЗРЕНИЯPOINT OF VIEWОбнаружение генов бета-глобина в эритроцитарной фракции у пациентки с раком шейки маткиDetection of beta-globin genes in the erythrocyte fraction in a patient with cervical cancerhttps://orcid.org/0000-0002-9243-6623КононоваИ. В.KononovaI. V.

Кононова Ирина Васильевна – к.м.н., в.н.с. 

Kononova Irina Vasilyevna – PhD in Medicine, associate researcher, acting director, Arctic Medical Center

irinakon.07@mail.ruМамаеваС. Н.MamaevaS. N.

Мамаева Саргылана Николаевна – к.ф.-м.н., зав. кафедрой Физ.-технич. ин-та 

Mamayeva Sargylana Nikolaevna – Ph.D. in Physics and Mathematics, head of the Department of the Institute of Physics and Technology

АлексеевВ. А.AlekseevV. A.

Алексеев Владислав Амирович – м.н.с.

Alekseev Vladislav Amirovich – junior researcher Arctic Medical Center

Якутский научный центр комплексных медицинских проблемРоссияYakut Science Centre for Complex Medical ProblemsRussian FederationСеверо-Восточный федеральный университет им. М.К. АммосовРоссияM.K. Ammosov Northeastern Federal UniversityRussian Federation20220702202504104107Copyright © Кононова И.В., Мамаева С.Н., Алексеев В.А., 20252025Кононова И.В., Мамаева С.Н., Алексеев В.А.Kononova I.V., Mamaeva S.N., Alekseev V.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/325

Представлены результаты детекции, проведенной с помощью полимеразной цепной реакции в реальном времени (ПЦР-РВ), фрагментов ДНК, кодирующих бета-глобин человека и белок L1 вируса папилломы человека, в образцах плазмы и эритроцитов у пациентки с раком шейки матки. Амплификация фрагмента ДНК, кодирующего бета-глобин человека, была обнаружена только в образце эритроцитов, что косвенно может свидетельствовать о наличии внеклеточной ДНК. Результаты ПЦР-РВ на наличие фрагмента ДНК, кодирующего L1 ВПЧ, были отрицательными, что не позволило подтвердить принадлежность выделенной ДНК к опухолевой. Происхождение идентифицированного в эритроцитарной фракции фрагмента ДНК, кодирующего бета-глобин человека, требует дальнейших исследований.

The results of detection of DNA fragments encoding human beta-globin and human papillomavirus L1 protein in plasma and erythrocyte samples from a cervical cancer patient using real-time polymerase chain reaction (qPCR) are presented. Amplification of a DNA fragment encoding human beta-globin was found only in a sample of erythrocytes, which may indirectly indicate the presence of extracellular DNA. The results of qPCR for the presence of a DNA fragment encoding HPV L1 were negative, which did not allow us to confirm that the isolated DNA belonged to the tumor DNA. The origin of the identified in the erythrocyte fraction DNA fragment encoding human beta-globin requires further research.

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The authors declare that there are no conflicts of interest present.