Pregnancy is accompanied by the formation of oxidative stress (OS), and a balance in the glutathione system as a universal component of antioxidant protection is important for the successful course of the gestational period. In order to identify the ecological and ethnic features of the glutathione defense system in the context of iron deficiency (ferritin < 30 ng/ml), 177 pregnant women from the migrant and indigenous populations of the Amur region were examined upon initial consultation. The content of hemoglobin, ferritin, total glutathione, reduced glutathione, oxidized glutathione, and redox status, a marker of OS, was determined in three groups: Caucasians, who were urban immigrants, and Mongoloids, who were rural immigrants. The ethnic characteristics of indigenous pregnant women were manifested in a significant increase in the oxidized form of glutathione and a tendency towards a decrease in the second trimester, which is characteristic only for indigenous pregnant women. The most favorable variant of the glutathione link of the ADS for the enhancement of FRO during pregnancy against the background of LID in newcomers from the village. The identified ecological and ethnic features indicate the need for an individual approach to pregnancy management.