ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМП10.25789/YMJ.2024.86.17ymj-286Research ArticleНАУЧНЫЕ ОБЗОРЫSCIENTIFIC REVIEWSСовременные методы экспериментальной оценки биомеханических свойств роговицыModern methods of experimental evaluation of biomechanical propertieshttps://orcid.org/0000-0001-7115-4275АветисовС. Э.AvetisovS. E.

Аветисов Сергей Эдуардович – д.м.н., проф., акад. РАН, научн. руковод. НИИ глазных болезней им. М.М. Краснова, зав. кафедрой Первого МГМУ им. И.М. Сеченова

Avetisov S.E. – MD, Professor, Academician of Russian Academy of Science, M.M. Krasnov Research Institute of Eye Diseases, Director on Science I.M. Sechenov First Moscow State Medical University, Department of Ophthalmology, Chief

https://orcid.org/0000-0002-2245-6346ДзамиховаА. К.DzamikhovaA. K.

ДзамиховА Асият Касумовна – аспирант

Dzamikhova A.K. – post-graduate student

asiat.abukerimova@yandex.ruhttps://orcid.org/0000-0001-8249-9703ШитиковаА. В.ShitikovaA. V.

Шитикова Алина Викторовна – ассистент кафедры

Shitikova A.V. – Assistant of the department

https://orcid.org/0000-0001-7040-253XЕфремовЮ. М.EfremovYu. M.

Ефремов Юрий Михайлович – к.б.н., зав. Отделом

Efremov YU.M. – PhD, Head of Department

https://orcid.org/0000-0001-7773-2435ТимашевП. С.TimashevP. S.

Тимашев Петр Сергеевич – д.х.н., проф., научн. руковод.

Timashev P.S. – Doctor of Chemical Sciences, Professor, Scientific Supervisor

НИИ глазных болезней им. М.М. Краснова; Первый МГМУ им. И.М. СеченоваРоссияM.M. Krasnov Research Institute of Eye Diseases; I.M. Sechenov First Moscow State Medical University, Department of OphthalmologyRussian FederationНИИ глазных болезней им. М.М. КрасноваРоссияM.M. Krasnov Research Institute of Eye DiseasesRussian FederationПервый МГМУ им. И.М. СеченоваРоссияM.M. Krasnov Research Institute of Eye DiseasesRussian FederationИн-т регенеративной медицины Первого МГМУРоссияI.M. Sechenov First Moscow State Medical UniversityRussian FederationИн-т регенеративной медицины Первый МГМУ им. И.М. СеченоваРоссияI.M. Sechenov First Moscow State Medical UniversityRussian Federation202406022025027075Copyright © Аветисов С.Э., Дзамихова А.К., Шитикова А.В., Ефремов Ю.М., Тимашев П.С., 20252025Аветисов С.Э., Дзамихова А.К., Шитикова А.В., Ефремов Ю.М., Тимашев П.С.Avetisov S.E., Dzamikhova A.K., Shitikova A.V., Efremov Y.M., Timashev P.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/286

В обзоре представлены описание методов и результаты различных экспериментальных исследований биомеханических свойств роговицы: методика растяжения образцов, способ индентации, а также атомно-силовая микроскопия. В качестве «источников» образцов рассмотрены роговицы экспериментальных животных и человека (в частности, донорских глаз и материала, полученного в результате кератопластики). Селективная оценка отдельных структур роговицы с помощью классических механических тестов на растяжение в известной степени лимитирована из-за достаточно малой толщины этих структур и, как следствие, сложностей фиксации образца. В реальной практике перспективным остается использование более адаптированных для проведения подобных исследований индентации и атомно-силового микроскопа, с одной стороны, исключающих необходимость механической фиксации образца, а с другой – обеспечивающих возможность исследования различных участков и поверхностей последнего.

The review presents a description of methods and results of various experimental studies of corneal biomechanical properties: the technique of tensile testing, the indentation method, and atomic force microscopy. Corneas of experimental animals and humans (in particular, donor eyes and material obtained as a result of keratoplasty) are considered as "sources" of samples. Selective evaluation of individual corneal structures using classical mechanical tensile tests is limited to a certain extent due to the rather small thickness of these structures and, as a consequence, difficulties in fixing the specimen. In real practice, it remains promising to use indentation and AFM, which are more adapted for such studies, on the one hand, eliminating the need for mechanical fixation of the specimen, and on the other hand, providing the possibility of studying various areas and surfaces of the latter.

роговицабиомеханические свойстваметоды экспериментальной оценкиcorneabiomechanical propertiesmethods of experimental evaluationРабота выполнена при поддержке гранта Российского научного фонда (грант № 23-74-10113, https://rscf.ru/project/23-74-10113/)ReferencesАветисов, Э. С. Близорукость. М.: Медицина,1986. 239 с.Avetisov E.S. Myopia. M.: Medicine, 1986. 239 p.Аветисов Э.С. Близорукость. 2-е изд. / Э.С. Аветисов М.: Медицина, 1999. 288 с.Avetisov E.S. Myopia. 2nd ed. / E.S. Avetisov. M.: Medicine, 1999. 288 p.Аветисов, Э. С. Трехфакторная теория близорукости и ее прогрессирования / Близорукость. Патогенез, профилактика прогрессирования и осложнений: материалы международ. симпоз. Москва, 1990. С.9-15.Avetisov, E. S. Three-factor theory of myopia and its progression / Myopia. Pathogenesis, prevention of progression and complications: materials of the international symposium. Moscow. 1990. Р: 9-15.Антонюк В.Д., Кузнецова Т.С. Исследование биомеханических свойств роговицы на приборе CORVIS ST (Oculus, Германия) у пациентов с миопией и миопическим астигматизмом / Офтальмохирургия. 2020. No. 4. 20 – 28 c.Antonyuk V.D., Kuznetsova T.S. Study of corneal biomechanical properties on CORVIS ST device (Oculus, Germany) in patients with myopia and myopic astigmatism / Ophthalmosurgery. 2020. No. 4. P. 20 28.Иомдина Е.Н., Бауэр С.М., Котляр К.Е. Биомеханика глаза: теоретические аспекты и клинические приложения / Москва: Реальное Время; 2015.Iomdina E.N., Bauer S.M., Kotliar K.E. Eye biomechanics: theoretical aspects and clinical applications. / Moscow: Real Time; 2015.Плескова С.Н. Атомно-силовая микроскопия в биологических и медицинских исследованиях: Учебное пособие / Долгопрудный: Издательский Дом «Интеллект». 2011. Р:184 с.Pleskova S.N. Atomic force microscopy in biological and medical research: Textbook / Dolgoprudny: 'Intellect' Publishing House. 2011. 184 p.Сравнительная оценка биомеханических характеристик ацеллюлярного дермального матрикса для герниопластики / К.И. Мелконян, К.И. Попандопуло, С.Б. Базлов [и др.] // Кубанский научный медицинский вестник. 2022. №29(5). – C:94-107. doi.org/10.25207/1608-6228-2022-29-5-94-107Comparative Evaluation of Biomechanical Characteristics of Acellular Dermal Matrix for Hernioplasty / K.I. Melkonyan, K.I. Popandopulo, S.B. Bazlov [et al.] // Kuban Scientific Medical Bulletin. 2022. №29(5). Р: 94-107. doi.org/10.25207/1608-6228-2022-29-5-94-107Экспериментальные исследования биомеханических свойств роговицы / С.Э. Аветисов, Г.А. Осипян, А.К. Абукеримова и др. // Вестник офтальмологии. 2022. №138(3). С.124-131. DOI10.17116/oftalma2022138031124.Experimental studies of the biomechanical properties of the cornea /s.E. Avetisov, G.A. Osipyan, A.K. Abukerimova [et al.] // Vestn Oftalmol. 2022. No.138(3). Р:124-131.A comparison of biomechanical properties between human and porcine cornea / Y. Zeng, J. Yang, K. Huang, Z. Lee, X. Lee // J Biomech. 2001. No. 34(4). Р: 533-7. doi: 10.1016/s0021-9290(00)00219-0A comparison of biomechanical properties between human and porcine cornea / Y. Zeng, J. Yang, K. Huang, Z. Lee, X. Lee // J Biomech. 2001. No. 34(4). Р: 533-7. doi: 10.1016/s0021-9290(00)00219-0A magnetically actuated, optically sensed tensile testing method for mechanical characterization of soft biological tissues / L. Rosalia, A. Hallou, L. Cochrane, T. Savin //sci Adv. 2023. No.13;9(2):eade2522. doi: 10.1126/sciadv.ade2522.A magnetically actuated, optically sensed tensile testing method for mechanical characterization of soft biological tissues / L. Rosalia, A. Hallou, L. Cochrane, T. Savin //sci Adv. 2023. No.13;9(2):eade2522. doi: 10.1126/sciadv.ade2522.Binnig G., Quate C.F., Gerber C. Atomic force microscope // Phys Rev Lett. 1986. No.3;56(9). Р:930-933. doi: 10.1103/PhysRevLett.56.930.Binnig G., Quate C.F., Gerber C. Atomic force microscope // Phys Rev Lett. 1986. No.3;56(9). Р:930-933. doi: 10.1103/PhysRevLett.56.930.Biomechanical Characterization of Human Soft Tissues Using Indentation and Tensile Testing / M. Griffin, Y. Premakumar, A. Seifalian // J Vis Exp. 2016. No.13;(118):54872. doi: 10.3791/54872.Biomechanical Characterization of Human Soft Tissues Using Indentation and Tensile Testing / M. Griffin, Y. Premakumar, A. Seifalian // J Vis Exp. 2016. No.13;(118):54872. doi: 10.3791/54872.Biomechanics of the anterior human corneal tissue investigated with Atomic Force Microscopy / M. Lombardo, G. Lombardo, G. Carbone, M.P. De Santo, R. Barberi, S. Serrao // Investigative Ophthalmology & Visual Science, published online. 2012. No.53(2). Р:1050-1057.Biomechanics of the anterior human corneal tissue investigated with Atomic Force Microscopy / M. Lombardo, G. Lombardo, G. Carbone, M.P. De Santo, R. Barberi, S. Serrao // Investigative Ophthalmology & Visual Science, published online. 2012. No.53(2). Р:1050-1057.Buch H. Prevalence and causes of visual impairment according to World Health Organization and United States criteria in an aged, urban Scandinavian population The Copenhagen City Eye Study // Ophthalmology. 2001. No.108(12). Р.2347-2357. doi: 10.1016/s0161-6420(01)00823-5Buch H. Prevalence and causes of visual impairment according to World Health Organization and United States criteria in an aged, urban Scandinavian population The Copenhagen City Eye Study // Ophthalmology. 2001. No.108(12). Р.2347-2357. doi: 10.1016/s0161-6420(01)00823-5Corneal Biomechanical Properties in Varying Severities of Myopia / M.R. Sedaghat, Н. Momeni-Moghaddam, А. Azimi [et al.] // Front Bioeng Biotechnol. 2021. No. 21(8):595330. doi:10.3389/fbioe.2020.595330.Corneal Biomechanical Properties in Varying Severities of Myopia / M.R. Sedaghat, Н. Momeni-Moghaddam, А. Azimi [et al.] // Front Bioeng Biotechnol. 2021. No. 21(8):595330. doi:10.3389/fbioe.2020.595330.Compliance profile of the human cornea as measured by atomic force microscopy / Last J, Sara MТ, Croasdale СR, Russell P, Murphy CJ. // Micron. 2012. No. 43(12). Р:1293-8. doi.org/10.1016/j.micron.2012.02.014].Compliance profile of the human cornea as measured by atomic force microscopy / Last J, Sara MТ, Croasdale СR, Russell P, Murphy CJ. // Micron. 2012. No. 43(12). Р:1293-8. doi.org/10.1016/j.micron.2012.02.014].Danielsen C.C. Tensile mechanical and creep properties of Descemet's membrane and lens capsule // Exp Eye Res. 2004. No. 79(3). P:343-50. doi.org/10.1016/j.exer.2004.05.014Danielsen C.C. Tensile mechanical and creep properties of Descemet's membrane and lens capsule // Exp Eye Res. 2004. No. 79(3). P:343-50. doi.org/10.1016/j.exer.2004.05.014Determination of elastic moduli of thin layers of soft material using the atomic force microscope / E.K. Dimitriadis, F. Horkay, J. Maresca, B. Kachar, R.S. Chadwick // Biophys J. 2002. No. 82(5). Р:2798-810. doi: 10.1016/S0006-3495(02)75620-8.Determination of elastic moduli of thin layers of soft material using the atomic force microscope / E.K. Dimitriadis, F. Horkay, J. Maresca, B. Kachar, R.S. Chadwick // Biophys J. 2002. No. 82(5). Р:2798-810. doi: 10.1016/S0006-3495(02)75620-8.Determining the mechanical properties of human corneal basement membranes with atomic force microscopy / J.A. Last, S.J. Liliensiek, P.F. Nealey, C.J. Murphy // Journal of Structural Biology. 2009. No.167. Р:19-24. doi.org/10.1016/j.jsb.2009.03.012Determining the mechanical properties of human corneal basement membranes with atomic force microscopy / J.A. Last, S.J. Liliensiek, P.F. Nealey, C.J. Murphy // Journal of Structural Biology. 2009. No.167. Р:19-24. doi.org/10.1016/j.jsb.2009.03.012Dias J.M, Ziebarth N.M. Anterior and posterior corneal stroma elasticity assessed using nanoindentation // Exp Eye Res. 2013. No.115. Р:41-46. doi.org/10.1016/j.exer.2013.06.004Dias J.M, Ziebarth N.M. Anterior and posterior corneal stroma elasticity assessed using nanoindentation // Exp Eye Res. 2013. No.115. Р:41-46. doi.org/10.1016/j.exer.2013.06.004Electromechanical probe and automated indentation maps are sensitive techniques in assessing early degenerated human articular cartilage /s. Sim, A. Chevrier, M. Garon [et al.] // J Orthop Res. 2017. No.35(4). P:858-867. doi: 10.1002/jor.23330.Electromechanical probe and automated indentation maps are sensitive techniques in assessing early degenerated human articular cartilage /s. Sim, A. Chevrier, M. Garon [et al.] // J Orthop Res. 2017. No.35(4). P:858-867. doi: 10.1002/jor.23330.Epidemiologic Study of Ocular Refraction among Schoolchildren in Taiwan in 1995 / L. Lin, Y. Shih, C. Tsai, [et al] // Optometry and Vision Science. 1999. No. 76(5). Р:275-281. doi:/10.1097/00006324-199905000-00013Epidemiologic Study of Ocular Refraction among Schoolchildren in Taiwan in 1995 / L. Lin, Y. Shih, C. Tsai, [et al] // Optometry and Vision Science. 1999. No. 76(5). Р:275-281. doi:/10.1097/00006324-199905000-00013Fisher R.F. Elastic constants of the human lens capsule // Physiol. 1969. No. 201. P:1-19. doi.org/10.1113/physiol.19.9.sp008739Fisher R.F. Elastic constants of the human lens capsule // Physiol. 1969. No. 201. P:1-19. doi.org/10.1113/physiol.19.9.sp008739Foster, C.S., Yamamoto, G.K. Ocular rigidity in keratoconus // American Journal of Ophthalmology. 1978. No.86. Р:802-806Foster, C.S., Yamamoto, G.K. Ocular rigidity in keratoconus // American Journal of Ophthalmology. 1978. No.86. Р:802-806Fung Y.C. Biomechanics. 10.1007/978-1-4757-2257-4. doi:10.1007/978-1-4757-2257-4Fung Y.C. Biomechanics. 10.1007/978-1-4757-2257-4. doi:10.1007/978-1-4757-2257-4Ganesan P, Wildsoet C. Pharmaceutical intervention for myopia control // Expert Rev Ophthalmol. 2010. No.5(6). Р:759-787. doi:10.1586/ eop.10.67Ganesan P, Wildsoet C. Pharmaceutical intervention for myopia control // Expert Rev Ophthalmol. 2010. No.5(6). Р:759-787. doi:10.1586/ eop.10.67Global Prevalence of Myopia and High Myopia and Temporal Trends from 2000 through 2050 / B.A. Holden, T.R. Fricke, D.A. Wilson [et al.] // Ophthalmology. 2016. No.123. Р:1036-1042. DOI: 10.1016/j.ophtha.2016.01.006Global Prevalence of Myopia and High Myopia and Temporal Trends from 2000 through 2050 / B.A. Holden, T.R. Fricke, D.A. Wilson [et al.] // Ophthalmology. 2016. No.123. Р:1036-1042. DOI: 10.1016/j.ophtha.2016.01.006Grading cartilage damage with diffuse reflectance spectroscopy: Optical markers and mechanical properties / N.R. Rovnyagina, G.S. Budylin, P.V. Dyakonov, Y.M. Efremov [et al.] // J Biophotonics. 2023. No.16(3):e202200149. doi: 10.1002/jbio.202200149.Grading cartilage damage with diffuse reflectance spectroscopy: Optical markers and mechanical properties / N.R. Rovnyagina, G.S. Budylin, P.V. Dyakonov, Y.M. Efremov [et al.] // J Biophotonics. 2023. No.16(3):e202200149. doi: 10.1002/jbio.202200149.Hawkes P.W., Spence J.C. Atomic Force Microscopy in the Life Sciences //science of Microscopy. 2007. No.10.1007/978-0-387-49762-4. P:1025–1069. doi:10.1007/978-0-387-49762-4_16Hawkes P.W., Spence J.C. Atomic Force Microscopy in the Life Sciences //science of Microscopy. 2007. No.10.1007/978-0-387-49762-4. P:1025–1069. doi:10.1007/978-0-387-49762-4_16Indentation versus tensile measurements of Young's modulus for soft biological tissues / C.T. McKee, J.A. Last, P.russell, C.J. Murphy // Tissue Eng Part B Rev. 2011. No.7(3). P:155-64. doi: 10.1089/ten.TEB.2010.0520.Indentation versus tensile measurements of Young's modulus for soft biological tissues / C.T. McKee, J.A. Last, P.russell, C.J. Murphy // Tissue Eng Part B Rev. 2011. No.7(3). P:155-64. doi: 10.1089/ten.TEB.2010.0520.International Conference on Mechatronics and Automation (ICMA) A cost-effective microindentation system for soft material characterization / W. Zhang, X. Dong [et al.] // 2015. P:825–830. doi:10.1109/icma.2015.7237592International Conference on Mechatronics and Automation (ICMA) A cost-effective microindentation system for soft material characterization / W. Zhang, X. Dong [et al.] // 2015. P:825–830. doi:10.1109/icma.2015.7237592S. The prevalence rates of refractive errors among children, adolescents, and adults in Germany // Clinical Ophthalmology. 2008. No.601. doi.org/10.2147/opth.s2836S. The prevalence rates of refractive errors among children, adolescents, and adults in Germany // Clinical Ophthalmology. 2008. No.601. doi.org/10.2147/opth.s2836Keratoconus: biomechanics ex vivo / R. Lohmüller, D. Böhringer, P.C. Maier, A.K. Ross [et al.] // Klin Monbl Augenheilkd. 2023. No. 240(6). P:774-778. doi: 10.1055/a-2062-3633Keratoconus: biomechanics ex vivo / R. Lohmüller, D. Böhringer, P.C. Maier, A.K. Ross [et al.] // Klin Monbl Augenheilkd. 2023. No. 240(6). P:774-778. doi: 10.1055/a-2062-3633Kontomaris S.V., Stylianou A. Atomic force microscopy for university students: applications in biomaterials // European Journal of Physics. 2017. No. 38(3);033003. doi:10.1088/1361-6404/aa5cd6Kontomaris S.V., Stylianou A. Atomic force microscopy for university students: applications in biomaterials // European Journal of Physics. 2017. No. 38(3);033003. doi:10.1088/1361-6404/aa5cd6Lavanya Devi A.L., Nongthomba U., Bobji M.S. Quantitative characterization of adhesion and stiffness of corneal lens of Drosophila melanogaster using atomic force microscopy // J Mech Behav Biomed Mater. 2016. No.53. Р:161-173.Lavanya Devi A.L., Nongthomba U., Bobji M.S. Quantitative characterization of adhesion and stiffness of corneal lens of Drosophila melanogaster using atomic force microscopy // J Mech Behav Biomed Mater. 2016. No.53. Р:161-173.Nanoscale topography of the corneal epithelial basement membrane and Descemet’s membrane of the human / G.A. Abrams, S.S. Schaus, S.L. Goodman, P.F. Nealey, C.J. Murphy // Cornea. 2000. No.19. Р:57–64. doi.org/10.1097/00003226-200001000-00012].Nanoscale topography of the corneal epithelial basement membrane and Descemet’s membrane of the human / G.A. Abrams, S.S. Schaus, S.L. Goodman, P.F. Nealey, C.J. Murphy // Cornea. 2000. No.19. Р:57–64. doi.org/10.1097/00003226-200001000-00012].New locus for autosomal dominant high myopia maps to the long arm of chromosome / P. Paluru, S.M. Ronan, E. Heon, M. Devoto, Wildenberg [et al.] // Invest. Ophthalmol. Vis. Sci. 2003. No. 44. Р:1830–1836. doi: 10.1167/iovs.02-0697New locus for autosomal dominant high myopia maps to the long arm of chromosome / P. Paluru, S.M. Ronan, E. Heon, M. Devoto, Wildenberg [et al.] // Invest. Ophthalmol. Vis. Sci. 2003. No. 44. Р:1830–1836. doi: 10.1167/iovs.02-0697Nguyen B.A., Roberts C.J., Reilly M.A. Biomechanical Impact of the Sclera on Corneal Deformation Response to an Air-Puff: A Finite-Element Study // Front Bioeng Biotechnol. 2019. No.10(6). Р:210. doi: 10.3389/fbioe.2018.00210.Nguyen B.A., Roberts C.J., Reilly M.A. Biomechanical Impact of the Sclera on Corneal Deformation Response to an Air-Puff: A Finite-Element Study // Front Bioeng Biotechnol. 2019. No.10(6). Р:210. doi: 10.3389/fbioe.2018.00210.Novel parameter of corneal biomechanics that differentiate normals from glaucoma / R. Lee, R.T. Chang, I.Y. Wong [et al.] // J. Glaucoma. 2016b. No. 25. P.603-609. doi: 10.1097/IJG.0000000000000284Novel parameter of corneal biomechanics that differentiate normals from glaucoma / R. Lee, R.T. Chang, I.Y. Wong [et al.] // J. Glaucoma. 2016b. No. 25. P.603-609. doi: 10.1097/IJG.0000000000000284Mechanical properties of anterior lens capsule assessed with AFM and nanoindenter in relation to human aging, pseudoexfoliation syndrome, and trypan blue staining / Y.M. Efremov, N.A. Bakhchieva, B.S. Shavkuta [et al.] // J Mech Behav Biomed Mater. 2020. No. 112:104081. doi: 10.1016/j.jmbbm.2020.104081.Mechanical properties of anterior lens capsule assessed with AFM and nanoindenter in relation to human aging, pseudoexfoliation syndrome, and trypan blue staining / Y.M. Efremov, N.A. Bakhchieva, B.S. Shavkuta [et al.] // J Mech Behav Biomed Mater. 2020. No. 112:104081. doi: 10.1016/j.jmbbm.2020.104081.Microindentation test for assessing the mechanical properties of cartilaginous tissues / X. Li, Y.H. An, Y.D. Wu // J Biomed Mater Res B Appl Biomater. 2007. No. 80(1). Р:25-31. doi: 10.1002/jbm.b.30564.Microindentation test for assessing the mechanical properties of cartilaginous tissues / X. Li, Y.H. An, Y.D. Wu // J Biomed Mater Res B Appl Biomater. 2007. No. 80(1). Р:25-31. doi: 10.1002/jbm.b.30564.Microindentation for in vivo measurement of bone tissue mechanical properties in humans / A. Diez-Perez, R. Güerri, X. Nogues [et al.] // J Bone Miner Res. 2010. No. 25(8). Р:1877-85. doi: 10.1002/jbmr.73.Microindentation for in vivo measurement of bone tissue mechanical properties in humans / A. Diez-Perez, R. Güerri, X. Nogues [et al.] // J Bone Miner Res. 2010. No. 25(8). Р:1877-85. doi: 10.1002/jbmr.73.Oyen M.L. Nanoindentation of Biological and Biomimetic Materials<span></span>, aop(aop). 2012. P:0–0. doi:10.1111/j.1747-1567.2011.00716.xOyen M.L. Nanoindentation of Biological and Biomimetic Materials<span></span>, aop(aop). 2012. P:0–0. doi:10.1111/j.1747-1567.2011.00716.xPrevalence and associated factors of myopia in high-school students in Beijing / L.J. Wu, Q.S. You, J.L. Duan [et al.] // PLoS ONE. 2015. No.10: e0120764. doi: 10.1371/journal.pone.0120764Prevalence and associated factors of myopia in high-school students in Beijing / L.J. Wu, Q.S. You, J.L. Duan [et al.] // PLoS ONE. 2015. No.10: e0120764. doi: 10.1371/journal.pone.0120764Probing Micromechanical Properties of the Extracellular Matrix of Soft Tissues by Atomic Force Microscopy / I. Jorba, J.J. Uriarte, N. R. Campillo, Farré, D. Navajas // J Cell Physiol. 2017. No. 232(1). P:19-26. doi: 10.1002/ jcp.25420Probing Micromechanical Properties of the Extracellular Matrix of Soft Tissues by Atomic Force Microscopy / I. Jorba, J.J. Uriarte, N. R. Campillo, Farré, D. Navajas // J Cell Physiol. 2017. No. 232(1). P:19-26. doi: 10.1002/ jcp.25420Sicard D., Fredenburgh L.E., Tschumperlin D.J. Measured pulmonary arterial tissue stiffness is highly sensitive to AFM indenter dimensions // J Mech Behav Biomed Mater. 2017. No.74. Р:118-127. doi: 10.1016/j.jmbbm.2017.05.039.Sicard D., Fredenburgh L.E., Tschumperlin D.J. Measured pulmonary arterial tissue stiffness is highly sensitive to AFM indenter dimensions // J Mech Behav Biomed Mater. 2017. No.74. Р:118-127. doi: 10.1016/j.jmbbm.2017.05.039.Soden P.D., Kershaw I. Tensile testing of connective tissues // Med Biol Eng. 1974. No.12(4). Р:510-8. doi: 10.1007/BF02478609.Soden P.D., Kershaw I. Tensile testing of connective tissues // Med Biol Eng. 1974. No.12(4). Р:510-8. doi: 10.1007/BF02478609.Softening Effects in Biological Tissues and NiTi Knitwear during Cyclic Loading / Y.F. Yasenchuk, E.S. Marchenko, S.V. Gunter [et al.] // Materials (Basel). 2021. No.21;14(21):6256. doi: 10.3390/ma14216256.Softening Effects in Biological Tissues and NiTi Knitwear during Cyclic Loading / Y.F. Yasenchuk, E.S. Marchenko, S.V. Gunter [et al.] // Materials (Basel). 2021. No.21;14(21):6256. doi: 10.3390/ma14216256.Standardized tensile testing of soft tissue using a 3D printed clamping system / M. Scholze, S. Safavi, K.C. Li, B. Ondruschka [et al.] // HardwareX. 2020. No. 21;8:e00159. doi:10.1016/j.ohx.2020.e00159.Standardized tensile testing of soft tissue using a 3D printed clamping system / M. Scholze, S. Safavi, K.C. Li, B. Ondruschka [et al.] // HardwareX. 2020. No. 21;8:e00159. doi:10.1016/j.ohx.2020.e00159.Tensile biomechanical properties and constitutive parameters of human corneal stroma extracted by SMILE procedure / Y. Xiang, M. Shen, C. Xue [et al.] // J Mech Behav Biomed Mater. 2018. No.85. Р:102-108. doi: 10.1016/j.jmbbm.2018.05.042.Tensile biomechanical properties and constitutive parameters of human corneal stroma extracted by SMILE procedure / Y. Xiang, M. Shen, C. Xue [et al.] // J Mech Behav Biomed Mater. 2018. No.85. Р:102-108. doi: 10.1016/j.jmbbm.2018.05.042.Use of Nanoindentation in Determination of Regional Biomechanical Properties of Rabbit Cornea After UVA Cross-Linking / X. Zheng, Y. Xin, C. Wang [et al.] // Invest Ophthalmol Vis Sci. 2023. No. 3;64(13). Р:26. doi:10.1167/iovs.64.13.26.Use of Nanoindentation in Determination of Regional Biomechanical Properties of Rabbit Cornea After UVA Cross-Linking / X. Zheng, Y. Xin, C. Wang [et al.] // Invest Ophthalmol Vis Sci. 2023. No. 3;64(13). Р:26. doi:10.1167/iovs.64.13.26.Vellara H.R., Patel D.V. Biomechanical properties of the keratoconic cornea: a review / Clin Exp Optom. 2015. No. 98(1). Р:31-8. doi: 10.1111/cxo.12211.Vellara H.R., Patel D.V. Biomechanical properties of the keratoconic cornea: a review / Clin Exp Optom. 2015. No. 98(1). Р:31-8. doi: 10.1111/cxo.12211.Wollensak G., Iomdina E. Long-term biomechanical properties of rabbit cornea after photodynamic collagen crosslinking / Acta Ophthalmol. 2009. No. 87(1). Р:48-51. doi: 10.1111/j.1755-3768.2008.01190.x.Wollensak G., Iomdina E. Long-term biomechanical properties of rabbit cornea after photodynamic collagen crosslinking / Acta Ophthalmol. 2009. No. 87(1). Р:48-51. doi: 10.1111/j.1755-3768.2008.01190.x.

The authors declare that there are no conflicts of interest present.