ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМП10.25789/YMJ.2023.83.17ymj-261Research ArticleАКТУАЛЬНАЯ ТЕМАACTUAL TOPICАнализ ассоциаций полиморфизма генов-кандидатов с развитием остеоартроза коленного сустава у больных с ожирениемAnalysis of associations of candidate gene polymorphism with the development of knee osteoarthritis in obese patientshttps://orcid.org/0000-0001-5337-2156НоваковВ. Б.NovakovV. B.

НОВАКОВ Виталий Борисович – врач травматолог-ортопед Городской больницы №2 г. Белгорода; аспирант мед. ин-та Белгородского гос. нац. иссл. ун-та

NOVAKOV Vitaly B. – orthopaedic surgeon, City Hospital №2 of Belgorod; postgraduate student, Belgorod State National Research University

v.novakov@bk.ruhttps://orcid.org/0000-0003-2700-1284НоваковаО. Н.NovakovaO. N.

НОВАКОВА Ольга Николаевна – к.б.н., доцент

NOVAKOVA Olga N. – PhD (Biol.), Associate Professor of the Department of Biomedical Sciences of the Medical Institute

litovkina@bsu.edu.ruhttps://orcid.org/0000-0003-1254-6134ЧурносовМ. И.ChurnosovM. I.

ЧУРНОСОВ Михаил Иванович – д.м.н., проф., зав. кафедрой мед. ин-та

CHURNOSOV Mikhail I. – MD (Med.), Professor, head of the Department of Biomedical Sciences of the Medical Institute

churnosov@bsu.eduГородская больница №2 г. Белгорода; Белгородский гос. нац. иссл. ун-тРоссияCity Hospital №2 of Belgorod; Belgorod State National Research UniversityRussian FederationБелгородский гос. нац. иссл. ун-тРоссияBelgorod State National Research UniversityRussian Federation202306022025036871Copyright © Новаков В.Б., Новакова О.Н., Чурносов М.И., 20252025Новаков В.Б., Новакова О.Н., Чурносов М.И.Novakov V.B., Novakova O.N., Churnosov M.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/261

Изучены ассоциации полиморфных локусов генов-кандидатов GDF5 (rs143384), NFAT5 (rs6499244), WWP2 (rs34195470), SBNO1 (rs1060105, rs56116847) с развитием остеоартроза коленного сустава у больных с ожирением.

Анализ ассоциаций изучаемых полиморфных локусов с развитием остеоартроза (ОА) коленного сустава у больных с ожирением выявил статистически значимые различия только для полиморфизма гена GDF5 (rs143384). Установлено, что частота генотипа G/G rs143384 у больных ОА коленного сустава с ожирением составила 14,12 %, что в 1,8 раза меньше по сравнению с группой контроля. Показана модифицирующая роль ожирения в характере ассоциаций полиморфного маркера rs143384 гена GDF5 с развитием ОА коленного сустава у населения Центрального Черноземья России. Выявлено, что генотип G/G rs143384 является протективным фактором развития заболевания у больных с ожирением.

Background

Background. Knee Osteoarthritis (OA) is a chronic disease with multifactorial pathogenesis. Risk factors for developing knee OA include age, genetic predisposition, and obesity. The share of genetic factors in the development of the disease accounts for up to 50 %. Despite the obvious association between obesity and knee OA, studies that reveal the role of genetic factors in the development of the disease in the interaction with obesity or overweight are extremely limited.

The aim of the study

The aim of the study: To study the association of candidate genes polymorphic loci GDF5 (rs143384), NFAT5 (rs6499244), WWP2 (rs34195470), SBNO1 (rs1060105, rs56116847) with the development of knee OA in patients with obesity.

Materials and methods

Materials and methods. The sample for the study included 322 obese individuals: 255 patients with OA of the knee and 67 people in the control group. Genotyping of DNA samples from all study participants was performed using standard real-time PCR on CFX96 amplifier (USA). Associations of genetic markers with knee OA in obese patients were assessed using the odds ratio and 95 % confidence interval.

Results

Results. Analysis of the associations of the studied polymorphic loci with the development of knee OA in obese patients revealed significant differences only for the GDF5 (rs143384) gene polymorphism. It was established that the frequency of the G/G genotype rs143384 in obese knee OA patients was 14.12 %, which is 1.8 times less compared to the control (25.36 %, p = 0.043, OR = 0.48).

Conclusions

Conclusions. The modifying role of obesity on the nature of the rs143384 polymorphic marker GDF5 gene associations with the developing knee OA in the population of the Russia Central Chernozem Region was shown. The G/G genotype rs143384 was found to be a protective factor in the development of knee OA in obese patients.

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The authors declare that there are no conflicts of interest present.