ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМП10.25789/YMJ.2024.86.12ymj-253Research ArticleГИГИЕНА, САНИТАРИЯ, ЭПИДЕМИОЛОГИЯ И МЕДИЦИНСКАЯ ЭКОЛОГИЯHYGIENE, SANITATION, EPIDEMIOLOGY AND MEDICAL ECOLOGYРоль полиморфизма GSTP1 lle105VAL в развитии аллергопатологии у детского населения индустриального центра Западного УралаThe role of lle105Val polymorphism of the gstp1 gene in the development of allergic pathology in the children population of the industrial center of Western Uralhttps://orcid.org/0000-0002-5162-9234СтарковаК. Г.StarkovaK. G.

Старкова Ксения Геннадьевна – к.б.н., зав. лаб.

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Starkova Ksenia Gennadievna – Head of the Laboratory of Immunology and Allergology, PhD in Biology

skg@fcrisk.ruhttps://orcid.org/0000-0003-4860-3145ДолгихО. В.DolgikhO. V.

Долгих Олег Владимирович – д.м.н., проф., зав. Отделом.

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Dolgikh Oleg Vladimirovich – Head of the Department of Immunobiological Diagnostic Methods

oleg@fcrisk.ruhttps://orcid.org/0000-0001-5850-7232АлексеевВ. Б.AlekseevV. B.

Алексеев Вадим Борисович – д.м.н., директор.

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Alekseev Vadim Borisovich – Director.

root@fcrisk.ruhttps://orcid.org/0000-0002-0114-3930КазаковаО. А.KazakovaO. A.

Казакова Ольга Алексеевна – к.б.н., зав. лаб.

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Kazakova Olga Alekseevna – Head of the Laboratory of Immunogenetics

chakina2011@yandex.ruhttps://orcid.org/0000-0002-1368-9703ЛегостаеваТ. А.LegostaevaT. A.

Легостаева Татьяна Андреевна – врач.

Пермь

Legostaeva Tatyana Andreevna – doctor of clinical laboratory diagnostics

ms.legota@mail.ruФБУН «ФНЦ медико-профилактических технологий управления рисками здоровью населения»РоссияFBSI Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”Russian Federation202406022025025053Copyright © Старкова К.Г., Долгих О.В., Алексеев В.Б., Казакова О.А., Легостаева Т.А., 20252025Старкова К.Г., Долгих О.В., Алексеев В.Б., Казакова О.А., Легостаева Т.А.Starkova K.G., Dolgikh O.V., Alekseev V.B., Kazakova O.A., Legostaeva T.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/253

Изучена роль полиморфизма Ile105Val гена GSTP1 (rs1695) в развитии аллергопатологии и его связь с особенностями формирования иммунного профиля у детского населения индустриального центра Западного Урала. Установлено, что аллель А и генотип AA полиморфного варианта Ile105Val гена GSTP1 у детей с аллергопатологией выступают маркерами чувствительности, ассоциированными с формированием аллергии, статистически значимым повышением IgE общего, эозинофилов крови и противовоспалительного цитокина IL-4, и могут рассматриваться в качестве перспективных индикаторов патофизиологических состояний, ассоциированных с риском развития атопических процессов (аллергопатологии) у детей, проживающих на Западном Урале.

A modern approach to the diagnostic support of patients with allergic diseases involves the introduction of innovative developments in the field of precision medicine, the development, identification and use of reliable biomarkers, including the determination of individual genetic variability in living conditions at various territories of the Russian Federation. The aim of the study is to investigate the role of the Ile105Val polymorphism of the GSTP1 gene (rs1695) in the development of allergic pathology and its connection with the characteristics of the formation of the immune profile in the child population of the industrial center of the Western Urals. Materials and methods. The observation group consisted of 34 children with allergic pathology. The comparison group included 37 children, relatively healthy, without allergies. Markers of hypersensitivity and cytokine status were studied using enzyme-linked immunosorbent assay. Genotyping was performed using real-time polymerase chain reaction. Results. In the group of children with allergic pathology, an increase in the eosinophilic-lymphocyte index was shown by 1.8 times, total IgE concentration by 4.7 times, a change in the level of serum cytokines IL-10 by 1.3 times and IL-4 by 1.8 times relative to the comparison group (p=0.005-0.038). Genetic analysis of the Ile105Val polymorphism of the GSTP1 gene revealed a 2.0-fold increase in the frequency of the homozygous AA genotype in the observation group (p=0.01). Allele A was associated with the development of allergies in the examined children (OR=2.36; 95% CI=1.16-4.79), while allele G had a protective value (OR=0.33; 95% CI= 0.12-0.89). An increase in the concentration of total IgE and blood eosinophils in carriers of the AA genotype was shown to be 4.6 times and 1.9 times, respectively, relative to owners of the AG and GG genotypes (p=0.013-0.031). Conclusion. Allele A and genotype AA of the polymorphic variant Ile105Val of the GSTP1 gene in children with allergic pathology act as sensitivity markers associated with the formation of allergies, a significant increase in total IgE, blood eosinophils and the anti-inflammatory cytokine IL-4, and can be considered as promising indicators of pathophysiological conditions associated with risk of development of atopic processes (allergic pathology) in children (RR=1.61; 95% CI=1.06-2.44) living in the Western Urals.

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The authors declare that there are no conflicts of interest present.