ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМПymj-2181Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHКоличественный анализ индукции фактора некроза опухоли клетками цельной крови ex vivo в присутствии антигена микобактерий туберкулезаQuantitative assessment of tumor necrosis factor induction by the whole blood cells ex vivo, in the presence of Mycobacterium tuberculosis antigensМордовскаяЛ. И.MordovskayaL. I.

Мордовская Лариса Ивановна – д.м.н., с.н.с.

Mordovskaya Larisa Ivanovna – MD, senior researcher

Sakha Republic (Yakutia)

limordovskaya@mail.ruВладимирскийМ. А.VladimirskiiM. A.

Владимирский Михаил Александрович – д.м.н., проф., руковод. лаб.

Vladimirskii Mikhail Alexandrovich – MD, Professor, Head of the Molecular diagnostics laboratory

Moscow

ШипинаЛ. К.ShipinaL. K.

Шипина Любовь Климовна – к.б.н., с.н.с.

Shipina Liubov’ Klimovna – Candidate of Biological Science, senior researcher, Molecular diagnostics laboratory

Moscow

АксеноваЕ. И.AksenovaE. I.

Аксенова Екатерина Ивановна – н.с.

Aksenova Ekaterina Ivanovna – researcher, Nanostructures laboratory

Moscow

СазыкинА. Ю.SazykinA. Yu.

Сазыкин Алексей Юрьевич – к.б.н., доцент

Sazykin Aleksei Yurievich – Candidate of Biological Science, associate professor, Department of Immunology

ГУ НПЦ «Фтизиатрия» МЗ РС (Я)РоссияState Institution “Research-Practice Center ‘Phthisiatry’ ”, Ministry of HealthRussian FederationНИИ фтизиопульмонологии Первого МГМУ им. И.М. СеченоваРоссияResearch Institute of Phthisiopulmonology, I.M. Sechenov First Moscow State Medical University (MSMU)Russian FederationНИИ фтизиопульмонологии Первого МГМУ им. и.м. СеченоваРоссияResearch Institute of Phthisiopulmonology I.M. Sechenov First Moscow State Medical University (MSMU)Russian FederationНИИ эпидемиологии и микробиологии им. Н.Ф. Гамалеи МЗ и СРРоссияGamaleya Research Institute of Epidemiology and Microbiology, Ministry of Health and Social Development of Russian FederationRussian FederationНИИ физико-химической биологии им. А.Н. Белозерского МГУ им. М.В. ЛомоносоваРоссияBelozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State UniversityRussian Federation201119102025043840Copyright © Мордовская Л.И., Владимирский М.А., Шипина Л.К., Аксенова Е.И., Сазыкин А.Ю., 20252025Мордовская Л.И., Владимирский М.А., Шипина Л.К., Аксенова Е.И., Сазыкин А.Ю.Mordovskaya L.I., Vladimirskii M.A., Shipina L.K., Aksenova E.I., Sazykin A.Y.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/2181

Изучена эффективность применения метода индукции фактора некроза опухоли (ФНО-α) в образцах цельной крови в присутствии антигенов микобактерий туберкулеза: туберкулина PPD и смеси специфических рекомбинантных антигенов ESAT-6 и CFP-10 для дифференциальной диагностики активной туберкулезной инфекции. Разработана собственная тест-система иммуноферментного анализа для количественного определения ФНО-α а образцах плазмы крови. На материалах исследований образцов крови 46 подростков с туберкулезным инфицированием (латентной инфекцией) и активным туберкулезом легких показана возможность дифференцирования активного туберкулеза от латентной инфекции при использовании антигенов ESAT-6 и CFP-10 в качестве индуктора ФНО-α.

We studied an efficacy of whole-blood method of tumor necrosis factor-alpha (TNF-alpha) induction in the presence of Mycobacterium tuberculosis antigens (tuberculin PPD and a mixture of specific recombinant ESAT-6 and CFP-10 antigens), for differential diagnosis of active tubercular infection. A customized test system with immunoenzyme assay was developed, to quantify TNF-alpha in plasma samples. Based on findings of blood sample investigations in 46 adolescents with tubercular infection (latent infection) and active pulmonary tuberculosis, the study demonstrated the possibility of differentiation between active and latent tubercular infection, using ESAT-6 and CFP-10 antigens as TNF-alpha inducers.

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The authors declare that there are no conflicts of interest present.