Заморщикова Ольга Михайловна – аспирант
Zamorschikova Olga Mikhailovna – postgraduate student of the Department of Infectious Diseases, Phthisiology and Dermatovenereology
Слепцова Снежана Спиридоновна – д.м.н., доцент, зав. кафедрой
Sleptsova Snezhana Spiridonovna – M.D., Associate Professor, Head of the Department of Infectious Diseases, Phthisiology and Dermatovenereology
Максимова Надежда Романовна – д.м.н., гл.н.с., руковод. НИЛ «Молекулярная медицина и генетика человека»
Maksimova Nadezhda Romanovna – M.D., chief researcher at Head of Research Laboratory "Molecular Medicine and Human Genetics"
Данилова Анастасия Лукинична – к.б.н., с.н.с. НИЛ «Молекулярная медицина и генетика человека»
Danilova Anastasia Lukinichna – Ph.D. in Biology, senior researcher at Research Laboratory "Molecular Medicine and Human Genetics"
Слепцов Спиридон Спиридонович – к.б.н., доцент; с.н.с.
Sleptsov spiridon spiridonovich – Ph.D. in Biology, associate professor, senior researcher at laboratories of clinical-population and medical-social researches
Петрова Любовь Иннокентьевна – к.м.н., доцент
Petrova Lyubov Innokentievna – Ph.D. in Medicine, Associate Professor of the Department of Infectious Diseases, Phthisiology and Dermatovenereology
Проведен анализ частоты встречаемости полиморфизмов rs8105790 гена IFNL3, rs368234815 гена IFNL4, rs1831583 гена IFNA1 среди этнической группы якутов, здоровых и больных хроническим вирусным гепатитом D, проживающих на территории Республики Саха (Якутия). HDV-инфекцией преимущественно страдают люди молодого трудоспособного возраста с быстрым формированием цирроза печени. Высокая репликативная активность вируса НDV сопровождается подавлением HBV, но с нарастанием тяжести фиброза и формированием осложнений наблюдается микст-репликация вирусов гепатита В и D. По результатам проведенного генетического исследования, риск развития тяжелого фиброза при HDV достоверно выше у носителей ΔG-аллеля полиморфизма rs368234815 гена IFNL4 и носителей С-аллеля полиморфизма rs1831583 гена IFNA1.
The aim of the research: to analyze the frequency of occurrence of polymorphisms rs8105790 of the IFNL3 gene, rs368234815 of the IFNL4 gene, rs1831583 of the IFNA1 gene in healthy people and patients with chronic viral hepatitis D among the ethnic group of Yakuts living on the territory of the Republic of Sakha (Yakutia).
Materials and methods of the study: to study gene polymorphisms in 157 individuals with chronic HDV infection and 160 apparently healthy individuals was used polymerase chain reaction (PCR). Analysis of the results included compliance with the Hardy-Weinberg law, Pearson's chisquared test (χ2), odds ratio and its confidence interval.
Results: the people of young working age suffer more from HDV infection, wherein the development of cirrhosis from the moment of infection with the D virus is formed on average over 6.5 years. The high replicative activity of the HDV virus in 74.1% of cases is accompanied by suppression of HBV, but with an increase in the severity of fibrosis and the formation of cirrhosis and liver cancer, there is observed simultaneous replication of hepatitis B and D viruses. According to the data obtained, the risk of developing severe fibrosis in HDV is 1.7 times higher in carriers of the ΔG-allele of the rs368234815 polymorphism of the IFNL4 gene (OR=1.784; 95% CI 0.642–4.959) and 1.8 times higher in the carriers of the C-allele of the rs1831583 polymorphism of the IFNA1 gene (OR= 1.818; 95% CI 0.340–9.713).
Conclusion: the obtained results demonstrate that the C-allele rs1831583 of the IFNA1 gene and the ΔG-allele rs368234815 of the IFNL4 gene predispose to the formation of severe fibrosis in HDV infection, Yakutia. Keywords: chronic hepatitis, liver cirrhosis, gene polymorphism, HDV, IFNL3, IFNL4, IFNA1.
The authors declare that there are no conflicts of interest present.