ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМПymj-1417Research ArticleСтатьиСтатус метилирования генов контроля клеточного цикла в тканях сонных артерий у больных атеросклерозомMethylation Status of the Cell Cycle Control-Associated Genes in the Carotid Artery Tissue of Atherosclerotic patientsНазаренкоМ. С.NazarenkoM. S.

Назаренко Мария Сергеевна – н.с. 

Maria S. Nazarenko, Ph.D 

10 Ushaika Embankment, Tomsk, 634050 

МарковА. В.MarkovA. V.

Марков Антон Владимирович – аспирант 

Anton V. Markov 

10 Ushaika Embankment, Tomsk, 634050 

КоролёваЮ. А.KorolevaI. A.

Королёва Юлия Александровна – ординатор 

 Iuliia A. Koroleva ccccccc

 10 Ushaika Embankment, Tomsk, 634050 

ЛебедевИ. Н.LebedevI. N.

Лебедев Игорь Николаевич – д.б.н., зав. лаб. 

 Igor N. Lebedev, Ph.D. 

 10 Ushaika Embankment, Tomsk, 634050 

СлепцовА. А.SleptcovA. A.

Слепцов Алексей Анатольевич – аспирант 

 Alexei A. Sleptcov 

 10 Ushaika Embankment, Tomsk, 634050 

ФроловА. В.FrolovA. V.

Фролов Алексей Витальевич – к.м.н., н.с. НИИ комплексных проблем сердечнососудистых заболеваний СО РАМН  

 Alexey V. Frolov, Ph.D. 

 6 Sosnovy Blvd, Kemerovo, 650002 

БарбарашО. Л.BarbarashO. L.

Барбараш Ольга Леонидовна – д.м.н., проф., директор НИИ комплексных проблем сердечно-сосудистых заболеваний СО РАМН 

 Olga L. Barbarash, Ph.D., MD 

 6 Sosnovy Blvd, Kemerovo, 650002 

ПузырёвВ. П.PuzyrevV. P.

Пузырёв Валерий Павлович – акад. РАМН, проф., директор 

 Valery P. Puzyrev Ph.D., MD 

 10 Ushaika Embankment, Tomsk, 634050 

НИИ медицинской генетики СО РАМНРоссияResearch Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical SciencesRussian FederationНИИ комплексных проблем сердечнососудистых заболеваний СО РАМН  РоссияResearch Institute for Complex Problems of Cardiovascular Diseases, Siberian Branch, Russian Academy of Medical SciencesRussian FederationНИИ комплексных проблем сердечно-сосудистых заболеваний СО РАМНРоссияResearch Institute for Complex Problems of Cardiovascular Diseases, Siberian Branch, Russian Academy of Medical SciencesRussian FederationРАМНРоссияResearch Institute of Medical Genetics, Siberian Branch, Russian Academy of Medical SciencesRussian Federation201414092025026163Copyright © Назаренко М.С., Марков А.В., Королёва Ю.А., Лебедев И.Н., Слепцов А.А., Фролов А.В., Барбараш О.Л., Пузырёв В.П., 20252025Назаренко М.С., Марков А.В., Королёва Ю.А., Лебедев И.Н., Слепцов А.А., Фролов А.В., Барбараш О.Л., Пузырёв В.П.Nazarenko M.S., Markov A.V., Koroleva I.A., Lebedev I.N., Sleptcov A.A., Frolov A.V., Barbarash O.L., Puzyrev V.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/1417

Проведен анализ статуса метилирования промоторов и первых экзонов генов CDKN2A (p16INK4a, p14ARF), CDKN2B (p15INK4b) и RB1 в 120 образцах сонных артерий с применением двух различных технологий: метилчувствительной полимеразной цепной реакции (МЧ-ПЦР) и метилспецифичной ПЦР (МС-ПЦР). Метилирование ДНК не было выявлено в выраженных атеросклеротических бляшках или прилежащих макроскопически неизменённых тканях сосудистой стенки тех же самых больных. Установлено, что статус метилирования генов CDKN2A, CDKN2B и RB1 не может быть использован в качестве маркера атеросклероза в сонных артериях человека.

Objective

Objective: We tested the hypothesis that the aberrant methylation of the promoters and first exons of CDKN2A (p16INK4a, p14ARF), CDKN2B (p15INK4b) and RB1 genes was associated with carotid atherosclerosis.

Methods and results

Methods and results: The DNA methylation status of these cell cycle control-associated genes was analysed in 120 samples of carotid arteries using two different techniques: methylationsensitive polymerase chain reaction (MS-PCR) and methylation-specific PCR (MSP-PCR). DNA methylation was not detected in advanced atherosclerotic plaques or nearby macroscopically intact tissues of the vascular wall from the same patients.

Conclusion

Conclusion: The methylation status of CDKN2A, CDKN2B and RB1 genes does not appear to be a marker of human carotid atherosclerosis.

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The authors declare that there are no conflicts of interest present.