ymjЯкутский медицинский журналYakut Medical Journal1813-19052312-1017ЯНЦ КМП10.25789/YMJ.2018.62.13ymj-1260Research ArticleМАТЕРИАЛЫ НАУЧНО-ПРАКТИЧЕСКОЙ КОНФЕРЕНЦИИ С МЕЖДУНАРОДНЫМ УЧАСТИЕМ «ВЛИЯНИЕ ХОЛОДА НА ОРГАНИЗМ ЧЕЛОВЕКА»MATERIALS OF SCIENTIFIC-PRACTICAL CONFERENCE WITH INTERNATIONAL PARTICIPATION «THE INFLUENCE OF COLD ON THE HUMAN ORGANISM»Анализ полиморфизма SNP-маркеров генов несократительного термогенеза UCP1 (rs1800592), UCP2 (rs659366) и UCP3 (rs2075577) у якутов и чукчейThe polymorphism of the SNP markers of the genes of non-reduced thermogenesis UCP1 (rs1800592), UCP2 (rs659366) and UCP3 (rs2075577) in the Yakuts and ChukchiНиканороваА. А.NikanorovaA. A.

НИКАНОРОВА Алена Афанасьевна – м.н.с., магистрант Института естественных наук

Nikanorova Alеna Afanasyevna – junior researcher of the Yakut Scientific Center of Complex Medical Problems, the Master’s Student

89244611585

nikanorova.alena@mail.ruБарашковН. А.BarashkovN. A.

БАРАШКОВ Николай Алексеевич – к.б.н., вед.н.с.-руковод. лаб.

Barashkov Nikolay Alekseyevich – Candidate of Biological Sciences, Leading Researcher - the Head of Laboratory

barashkov2004@mail.ruДьяконовЕ. Е.DiakonovE. E.

ДЬЯКОНОВ Егор Егорович – студент

Diakonov Egor Egorovich – student

diakonov2007@mail.ruНаходкинС. С.NakhodkinS. S.

НАХОДКИН Сергей Сергеевич – инженер-исследователь

Nakhodkin Sergey Sergeyevich – Engineer-researcher of the Laboratory of Molecular Biology

sergnahod@mail.ruПшенниковаВ. Г.PshennikovaV. G.

ПШЕННИКОВА Вера Геннадиевна – к.б.н., н.с.; Институт естественных наук

Pshennikova Vera Gennadiyevna – Candidate of Biological Sciences, Research Associate

СоловьевА. В.SolovyevA. V.

СОЛОВЬЕВ Айсен Васильевич – аспирант

Solovyev Aisen Vasilyevich – Postgraduate Student

nelloann@mail.ruКузьминаС. С.KuzminaS. S.

КУЗЬМИНА Саргылана Семеновна – к.б.н., доцент

Kuzmina Sargylana Semenovna – Candidate of Biological Sciences, Associate Professor, Department of Biology

sskuzmina@bk.ruТомскийМ. И.TomskyM. I.

ТОМСКИЙ Михаил Иннокентьевич – д.м.н., гл. врач 

Tomsky Mikhail Innokentyevich – Doctor of Medical Sciences, Acting as Director

ogus@list.ruБурцеваТ. Е.BurtsevaT. E.

БУРЦЕВА Татьяна Егоровна – д.м.н., проф.

Burtseva Tatiana Egorovna – Doctor of Medical Sciences, Professor

ФедороваС. А.FedorovaS. A.

ФЕДОРОВА Сардана Аркадьевна – д.б.н., зав. лаб.

Fedorova Sardana Arkadyevna – Doctor of Biological Sciences, Head of the Laboratory of Molecular Biology

sardaanafedorova@mail.ruФГБНУ «ЯНЦ КМП»; СВФУ им. М.К. АммосоваРоссияInstitute of Natural Science, NEFU M.K. AmmosovRussian FederationФГБНУ «ЯНЦ КМП»РоссияYakut Scientific Center of Complex Medical ProblemsRussian FederationФГБНУ «ЯНЦ КМП»; СВФУ им. М.К. АммосоваРоссияInstitute of Natural Sciences NEFU. M.K. AmmosovRussian FederationФГБНУ «ЯНЦ КМП»РоссияInstitute of Natural Sciences NEFU M.K. AmmosovRussian FederationФГБНУ «ЯНЦ КМП»; СВФУ им. М.К. АммосоваРоссияYakut Scientific Center of Complex Medical ProblemsRussian FederationФГБНУ «ЯНЦ КМП»РоссияInstitute of Natural Science, NEFU M.K. AmmosovRussian FederationФГБНУ «ЯНЦ КМП»; ГБУ «Республиканский детский туберкулезный санаторий им. Т.П. Дмитриевой»РоссияYakut Scientific Center of Complex Medical ProblemsRussian FederationФГБНУ «ЯНЦ КМП»; МИ СВФУРоссияMedical Institute of NEFU M.K. AmmosovRussian Federation201809092025024145Copyright © Никанорова А.А., Барашков Н.А., Дьяконов Е.Е., Находкин С.С., Пшенникова В.Г., Соловьев А.В., Кузьмина С.С., Томский М.И., Бурцева Т.Е., Федорова С.А., 20252025Никанорова А.А., Барашков Н.А., Дьяконов Е.Е., Находкин С.С., Пшенникова В.Г., Соловьев А.В., Кузьмина С.С., Томский М.И., Бурцева Т.Е., Федорова С.А.Nikanorova A.A., Barashkov N.A., Diakonov E.E., Nakhodkin S.S., Pshennikova V.G., Solovyev A.V., Kuzmina S.S., Tomsky M.I., Burtseva T.E., Fedorova S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ymj.elpub.ru/jour/article/view/1260

Впервые был проведен анализ частоты аллелей полиморфизма генов UCP1-rs1800592, UCP2-rs659366 и UCP3-rs2075577 в популяциях якутов (n=281) и чукчей (n=39), проживающих в экстремальных климатических условиях Восточной Сибири. Выявлена повышенная частота аллеля а rs2075577 гена UCP3 в популяциях якутов и чукчей, проживающих в условиях низких температур, что может быть связано со случайными популяционными эффектами или же свидетельствует о наличии адаптационных механизмов, связанных с терморегуляцией.

For the first time an analysis of the frequencies of alleles polymorphismof the gene UCP1-rs1800592, UCP2-rs659366 and UCP3-rs2075577were studiedthe Yakuts (n=281) and the Chukchi’s (n=39) populations, living in the extreme climate of Eastern Siberia.The Yakut population was divided into three groups: northern (N.YAK), viluysk (V.YAK) and central Yakuts groups (C.YAK).From project of «1000 Genomes», information was received on the frequencies of the studied polymorphisms for Chinese – CHB (n=103), CHS (n=108), CDX (n=99), Japanese – JPT (n=104), Vietnamese populations– KHV (n=101).In general, the frequencies of the studied polymorphisms were analyzed in nine populations of Asia (n=835), living in different climatic zones. Populations living in the subarctic (CHU, N.YAK) and on the border of the temperate and subarctic climate (V.YAK, C.YAK) were merged into the group «Northern Asia». Populations living in the temperate (CHB), subtropical (JPT; CHS; CDX) and subequatorial climate (KHV) were joined into the «Southern Asia» group. Comparative analysis showed that the frequency of the allele A rs1800592 (UCP1) in the group «Northern Asia» (61.8%, CI: 56.8-66.7%), did not statistically differ (p>0.01) from the «Southern Asia» group (53.4%, CI: 49.4-57.4%). The frequencies of allele T rs659366 (UCP2) in the group «Northern Asia» (49.5%, CI: 44.5-54.6%), as compared with the group «Southern Asia» (41.5%, CI: 37.7-45.5%), also statistically differences not found (p>0.01). It was found that the frequency of allele A rs2075577 (UCP3) in the group «Northern Asia» (66.7%, CI: 61.8-71.4%) was much higher in comparison with the group «Southern Asia» (42.3%, CI: 38.4-46.3%) (p<0.01). The increased frequency of allele A rs2075577 of the UCP3 gene in populations of Yakuts and Chukchi’s living in low temperature conditions, in comparison with other populations of Asia, can be associated with random population effects, or indicate the presence of adaptation mechanisms associated with thermoregulation.

бурая жировая тканьнесократительный термогенезразобщающие белкиген UCP1ген UCP2ген UCP3адаптацияхолодный климатпопуляция якутовпопуляция чукчейbrown adipose tissuenonshivering thermogenesisuncoupling proteinsUCP1 geneUCP2 geneUCP3 geneadaptationcold climateYakut populationChukchi populationРабота выполнена при финансовой поддержке госзадания Минобрнауки РФ №6.1766.2017.ПЧ, проекта СВФУ им. М.К. Аммосова «Генетические особенности населения Якутии: структура генофонда, адаптация к холоду, психогенетические характеристики, распространенность некоторых наследственных и инфекционных заболеваний» и программы биоресурсных коллекций ФАНО России УНУ «Геном Якутии» ЯНЦ КМП (БРК: 0556-2017-0003).This study was supported by the Ministry of Education and Science of the Russian Federation №6.1766.2017, the project NEFU M.K. 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The authors declare that there are no conflicts of interest present.